Reviewed, UniProtKB/Swiss-Prot Q9KKW6 (LLDD_VIBCH)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase [cytochrome]
    EC=1.1.2.3

Gene names

Name:	lldD
Ordered Locus Names:	VC_A0984

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+. HAMAP MF_01559
Cofactor	FMN By similarity. HAMAP MF_01559
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	L-lactate dehydrogenase [cytochrome] HAMAP MF_01559		PRO_0000206352
Regions
	Domain	1 – 378	378	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9KKW6-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8310DF5B3F217ADC	FASTA	378	41,291
	10 20 30 40 50 60 MIISASTDYR AAAKAKLPPF LFHYIDGGSY GEHTLRRNTD DLADIALRQR VLSDMSELSL 70 80 90 100 110 120 ETELFGEKMA LPIALSPVGL TGMYARRGEV QAAQAAEAKG IPFTLSTVSV CPIEEVAPSI 130 140 150 160 170 180 HRPIWFQLYV LKDRGFMKNV LERAKAAGVK NLVFTVDMPV PGARYRDMHS GMSGPNAAMR 190 200 210 220 230 240 RVLQAMAHPS WAWDVGLLGK PHDLGNISKY RGSPTKLEDY IGWLGANFDP SISWKDLEWI 250 260 270 280 290 300 RDFWDGPMII KGILDTEDAK DAVRFGADGI VVSNHGGRQL DGVLSTVQAL PAIADAVKGD 310 320 330 340 350 360 LKILVDSGIR TGLDVVRMLA LGADCTMLGR SFIYALAAQG RAGVENLLDL YEKEMRVAMT 370 LTGAKSIAEL SRDSLVKR

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003853 Genomic DNA. Translation: AAF96880.1.
PIR	B82392.
RefSeq	NP_233368.1.
3D structure databases
SMR	Q9KKW6. Positions 1-377.
ModBase	Search...
Genome annotation databases
GeneID	2612817.
GenomeReviews	Gene locus VC_A0984 in contig AE003853_GR.
KEGG	vch:VCA0984.
TIGR	VC_A0984.
Phylogenomic databases
HOGENOM	HBG517781.
OMA	WIREFWE.
Enzyme and pathway databases
BRENDA	1.1.2.3. 19019.
Family and domain databases
HAMAP	MF_01559. L-lact_dehydr. [Tree]
InterPro	IPR012133. a-Hydoxy_acid_DH_FMN. IPR013785. Aldolase_TIM. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. IPR020920. L-lactate_DHase_cyt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_VIBCH

Accession

Primary (citable) accession number: Q9KKW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)