Reviewed, UniProtKB/Swiss-Prot Q9KKQ7 (PTM3C_VIBCH)

Names and origin

Protein names

Recommended name:
    PTS system mannitol-specific EIICBA component
Alternative name(s):
    EIICBA-Mtl
      Short name=EII-Mtl
Including the following 3 domains:
    1- Recommended name:
            Mannitol permease IIC component
        Alternative name(s):
            PTS system mannitol-specific EIIC component
    2- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system mannitol-specific EIIB component
    3- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system mannitol-specific EIIA component

Gene names

Name:	mtlA
Ordered Locus Names:	VC_A1045

Organism

Vibrio cholerae [Complete proteome] [HAMAP]

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Post-translational modification	An intramolecular phosphotransfer takes places between His-564 and Cys-390 By similarity.
Sequence similarities	Contains 1 PTS EIIA type-2 domain. Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	PTS system mannitol-specific EIICBA component		PRO_0000186617
Regions
	Transmembrane	25 – 45	21	Potential
	Transmembrane	49 – 69	21	Potential
	Transmembrane	84 – 104	21	Potential
	Transmembrane	134 – 154	21	Potential
	Transmembrane	162 – 182	21	Potential
	Transmembrane	214 – 234	21	Potential
	Transmembrane	246 – 266	21	Potential
	Transmembrane	270 – 290	21	Potential
	Transmembrane	313 – 333	21	Potential
	Domain	13 – 342	330	PTS EIIC type-2
	Domain	384 – 475	92	PTS EIIB type-2
	Domain	504 – 646	143	PTS EIIA type-2
Sites
	Active site	390	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	564	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KKQ7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D2C67C27D8BE99CA	FASTA	649	68,674
	10 20 30 40 50 60 MISSDAKVKI QNFGRFLSNM VMPNIGAFIA WGFITALFIP TGWVPNETLA SLVGPMITYL 70 80 90 100 110 120 LPLLIGYTGG KLAGGERGAV VGAITTMGVI VGTDIPMFMG AMIVGPMGGW AIKAFDKKID 130 140 150 160 170 180 GKVRSGFEML VNNFSAGIIG MLCAIIAFFL IGPFVKVLSG ALAAGVNFLV TAHLLPLTSI 190 200 210 220 230 240 FVEPAKILFL NNAINHGIFS PLGIQQASET GQSIFFLIEA NPGPGLGILL AYMVFGKGTA 250 260 270 280 290 300 RQTAGGATII HFFGGIHEIY FPYILMNPRL ILAAIAGGMT GVFTLTVFNA GLVSPASPGS 310 320 330 340 350 360 IFAVLLMTNK GSILGVVCSI FAAAAVSFTV AALLMKAQTS TEQDGDKDAL VKATSIMQEM 370 380 390 400 410 420 KAGSKGQAAP TATQSKKIDM ANVQSIIVAC DAGMGSSAMG ASMLRKKIQE VGLPVTVTNM 430 440 450 460 470 480 AINSLPAHVD MVITHQDLTD RARQHAPNAE HISLNNFLDS ALYNQLVTQL LAAKRQAAND 490 500 510 520 530 540 SQLIKPSILA ANDDRYEVQQ PSVFQLQKEN IHLGLNAKNK EEAIRFAGNK LVELGYVHPE 550 560 570 580 590 600 YVDAMFEREK LVSTYLGESI AVPHGTVDAK DRVIKTGIVI CQYPQGVAFS EDSGDVAKLV 610 620 630 640 IGIAAKNDEH IQVITTITNA LDDPNAIDKL TSTKDVSDVL SILATSQAA

References

[1]

"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003853 Genomic DNA. Translation: AAF96939.1.
PIR	B82385.
RefSeq	NP_233427.1.
3D structure databases
HSSP	HSSP built from PDB template 1A3A based on UniProtKB P00550.
ModBase	Search...
Genome annotation databases
GeneID	2612120.
GenomeReviews	Gene locus VC_A1045 in contig AE003853_GR.
KEGG	vch:VCA1045.
TIGR	VC_A1045.
Phylogenomic databases
HOGENOM	Q9KKQ7.
OMA	ITHKDLT.
Enzyme and pathway databases
BRENDA	2.7.1.69. 19019.
Family and domain databases
InterPro	IPR002178. PTS_EIIA_2. IPR013011. PTS_EIIB_2. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR003501. PTS_IIB_lac. IPR004718. PTS_IIC_mtl. [Graphical view]
Gene3D	G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.
Pfam	PF00359. PTS_EIIA_2. 1 hit. PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
ProDom	PD001689. PTS_EIIA_2. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00851. mtlA. 1 hit.
PROSITE	PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTM3C_VIBCH

Accession

Primary (citable) accession number: Q9KKQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)