Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9KJY8

- PCS_RHIME

UniProt

Q9KJY8 - PCS_RHIME

Protein

Phosphatidylcholine synthase

Gene

pcs

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Condenses choline with CDP-diglyceride to produce phosphatidylcholine and CMP.4 Publications

    Catalytic activityi

    CDP-diacylglycerol + choline = CMP + phosphatidylcholine.4 Publications

    Cofactori

    Manganese.1 Publication

    Enzyme regulationi

    Activated by CDP-diacylglycerol especially in the presence of Triton X-100 (0.1% w/v) at concentrations where micelles are formed. Maximal activation by Triton X-100 at 0.2% w/v, but higher concentrations become inhibitory. Inhibited by EDTA and high concentrations of choline.1 Publication

    pH dependencei

    Optimum pH is 8.0 in cell-free extracts. Activity decreases as pH is raised or lowered.1 Publication

    GO - Molecular functioni

    1. phosphatidylcholine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. phospholipid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    Manganese

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16699.
    SMEL266834:GJF6-1713-MONOMER.
    BRENDAi2.7.8.24. 5715.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylcholine synthase (EC:2.7.8.24)
    Short name:
    PC synthase
    Short name:
    PCS
    Alternative name(s):
    CDP-diglyceride-choline O-phosphatidyltransferase
    Gene namesi
    Name:pcs
    Ordered Locus Names:R01672
    ORF Names:SMc00247
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001976: Chromosome

    Subcellular locationi

    Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi20 – 201H → A: Loss of enzyme activity. 1 Publication
    Mutagenesisi20 – 201H → L: 32% of wild-type enzyme activity. 1 Publication
    Mutagenesisi23 – 231T → A: Loss of enzyme activity. 1 Publication
    Mutagenesisi23 – 231T → S: 63% of wild-type enzyme activity. 1 Publication
    Mutagenesisi56 – 561D → A, E or N: Loss of enzyme activity. 1 Publication
    Mutagenesisi59 – 591D → A, E or N: Loss of enzyme activity. 1 Publication
    Mutagenesisi60 – 601G → A: Loss of enzyme activity. 1 Publication
    Mutagenesisi77 – 771G → A: Strongly reduced enzyme activity. 1 Publication
    Mutagenesisi81 – 811D → A: Loss of enzyme activity. 1 Publication
    Mutagenesisi81 – 811D → E or N: Strongly reduced enzyme activity. 1 Publication
    Mutagenesisi85 – 851D → A, E or N: Loss of enzyme activity. 1 Publication
    Mutagenesisi123 – 1231Y → A: Strongly reduced enzyme activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 241241Phosphatidylcholine synthasePRO_0000056812Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi266834.SMc00247.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9KJY8.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini37 – 415Periplasmic1 Publication
    Topological domaini63 – 7614Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini98 – 1003Periplasmic1 Publication
    Topological domaini122 – 13312Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini155 – 1562Periplasmic1 Publication
    Topological domaini178 – 18710Cytoplasmic1 Publication
    Topological domaini209 – 2113Periplasmic1 Publication
    Topological domaini233 – 2419Cytoplasmic1 Publication

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621Helical; Name=1CuratedAdd
    BLAST
    Transmembranei42 – 6221Helical; Name=2CuratedAdd
    BLAST
    Transmembranei77 – 9721Helical; Name= 3CuratedAdd
    BLAST
    Transmembranei101 – 12121Helical; Name=4CuratedAdd
    BLAST
    Transmembranei134 – 15421Helical; Name=5CuratedAdd
    BLAST
    Transmembranei157 – 17721Helical; Name=6CuratedAdd
    BLAST
    Transmembranei188 – 20821Helical; Name=7CuratedAdd
    BLAST
    Transmembranei212 – 23221Helical; Name=8CuratedAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1183.
    HOGENOMiHOG000066428.
    KOiK01004.
    OMAiYVFVPAY.
    OrthoDBiEOG6P5ZJN.

    Family and domain databases

    InterProiIPR000462. CDP-OH_P_trans.
    IPR026027. PcS.
    [Graphical view]
    PfamiPF01066. CDP-OH_P_transf. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000851. PcS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9KJY8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFFNYRRVP YAEIRAFSVH ILTASGSFLA FLGVVAAAEH RFVDMFWWLG    50
    LALLVDGIDG PIARKVQVKE VLPNWSGDTL DNVIDYVTYV LLPAFALYQS 100
    GMIGEPWSFV AAGAIVVSSA IYYADMGMKT DEYFFSGFPV VWNMVVFTLF 150
    VIQASEVTAS IVVFLSVILT FLPINFLHPV RVKRLRPLNL GIFLVWSVLG 200
    MYALLLHFET PPWVVVGVVA TGLYLYVIGF ILQIFPKLGR A 241
    Length:241
    Mass (Da):27,003
    Last modified:October 1, 2000 - v1
    Checksum:i864BC6E353D08D1B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF155772 Genomic DNA. Translation: AAF27310.1.
    AL591688 Genomic DNA. Translation: CAC46251.1.
    RefSeqiNP_385778.1. NC_003047.1.
    WP_003533217.1. NC_003047.1.

    Genome annotation databases

    EnsemblBacteriaiCAC46251; CAC46251; SMc00247.
    GeneIDi1233331.
    KEGGisme:SMc00247.
    PATRICi23632719. VBISinMel96828_3105.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF155772 Genomic DNA. Translation: AAF27310.1 .
    AL591688 Genomic DNA. Translation: CAC46251.1 .
    RefSeqi NP_385778.1. NC_003047.1.
    WP_003533217.1. NC_003047.1.

    3D structure databases

    ProteinModelPortali Q9KJY8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266834.SMc00247.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC46251 ; CAC46251 ; SMc00247 .
    GeneIDi 1233331.
    KEGGi sme:SMc00247.
    PATRICi 23632719. VBISinMel96828_3105.

    Phylogenomic databases

    eggNOGi COG1183.
    HOGENOMi HOG000066428.
    KOi K01004.
    OMAi YVFVPAY.
    OrthoDBi EOG6P5ZJN.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16699.
    SMEL266834:GJF6-1713-MONOMER.
    BRENDAi 2.7.8.24. 5715.

    Family and domain databases

    InterProi IPR000462. CDP-OH_P_trans.
    IPR026027. PcS.
    [Graphical view ]
    Pfami PF01066. CDP-OH_P_transf. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000851. PcS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the gene for phosphatidylcholine synthase."
      Sohlenkamp C., de Rudder K.E.E., Roehrs V., Lopez-Lara I.M., Geiger O.
      J. Biol. Chem. 275:18919-18925(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY.
      Strain: 1021.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    4. "Plant-exuded choline is used for rhizobial membrane lipid biosynthesis by phosphatidylcholine synthase."
      de Rudder K.E., Sohlenkamp C., Geiger O.
      J. Biol. Chem. 274:20011-20016(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: 1021.
    5. "Pathways for phosphatidylcholine biosynthesis in bacteria."
      Martinez-Morales F., Schobert M., Lopez-Lara I.M., Geiger O.
      Microbiology 149:3461-3471(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
      Strain: 1021.
    6. "Functional and topological analysis of phosphatidylcholine synthase from Sinorhizobium meliloti."
      Solis-Oviedo R.L., Martinez-Morales F., Geiger O., Sohlenkamp C.
      Biochim. Biophys. Acta 1821:573-581(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF HIS-20; THR-23; ASP-56; ASP-59; GLY-60; GLY-77; ASP-81; ASP-85 AND TYR-123.
      Strain: 1021.

    Entry informationi

    Entry nameiPCS_RHIME
    AccessioniPrimary (citable) accession number: Q9KJY8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3