Q9KJY8 (PCS_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylcholine synthase
Short name=PC synthase
EC=2.7.8.24

Alternative name(s):
CDP-diglyceride-choline O-phosphatidyltransferase

Gene names

Name:	pcs
Ordered Locus Names:	R01672
ORF Names:	SMc00247

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Rhizobium meliloti

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Condenses choline with CDP-diglyceride to produce phosphatidylcholine and CMP.
Catalytic activity	CDP-diacylglycerol + choline = CMP + phosphatidylcholine.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the CDP-alcohol phosphatidyltransferase class-I family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphatidylcholine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Phosphatidylcholine synthase		PRO_0000056812
Regions
	Transmembrane	16 – 38	23	Helical; Potential
	Transmembrane	102 – 124	23	Helical; Potential
	Transmembrane	133 – 152	20	Helical; Potential
	Transmembrane	157 – 179	23	Helical; Potential
	Transmembrane	186 – 208	23	Helical; Potential
	Transmembrane	213 – 235	23	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9KJY8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 864BC6E353D08D1B	FASTA	241	27,003
	10 20 30 40 50 60 MKFFNYRRVP YAEIRAFSVH ILTASGSFLA FLGVVAAAEH RFVDMFWWLG LALLVDGIDG 70 80 90 100 110 120 PIARKVQVKE VLPNWSGDTL DNVIDYVTYV LLPAFALYQS GMIGEPWSFV AAGAIVVSSA 130 140 150 160 170 180 IYYADMGMKT DEYFFSGFPV VWNMVVFTLF VIQASEVTAS IVVFLSVILT FLPINFLHPV 190 200 210 220 230 240 RVKRLRPLNL GIFLVWSVLG MYALLLHFET PPWVVVGVVA TGLYLYVIGF ILQIFPKLGR A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the gene for phosphatidylcholine synthase." Sohlenkamp C., de Rudder K.E.E., Roehrs V., Lopez-Lara I.M., Geiger O. J. Biol. Chem. 275:18919-18925(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: 1021.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF155772 Genomic DNA. Translation: AAF27310.1. AL591688 Genomic DNA. Translation: CAC46251.1.
RefSeq	NP_385778.1. NC_003047.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SMc00247.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC46251; CAC46251; SMc00247.
GeneID	1233331.
KEGG	sme:SMc00247.
PATRIC	23632719. VBISinMel96828_3105.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1183.
HOGENOM	HOG000066428.
KO	K01004.
OMA	FLHPFRV.
ProtClustDB	CLSK863002.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16699. SMEL266834:GJF6-1713-MONOMER.
BRENDA	2.7.8.24. 5715.
Family and domain databases
InterPro	IPR000462. CDP-OH_P_trans. IPR026027. PcS. [Graphical view]
Pfam	PF01066. CDP-OH_P_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000851. PcS. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PCS_RHIME

Accession

Primary (citable) accession number: Q9KJY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program