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Q9KJX5

- ALPH_ELIME

UniProt

Q9KJX5 - ALPH_ELIME

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Protein

Alkaline phosphatase PafA

Gene

pafA

Organism
Elizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Alkaline phosphatase with broad substrate specificity. Has phosphatase activity towards nucleotide phosphates with a preference for ATP. Active towards a great variety of phosphomonoesters with the exception of 2',3'-cyclic AMP and myo-inositol hexakisphosphate.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn(2+) ions.By similarity

Enzyme regulationi

Strongly inhibited by orthovanadate and EDTA. Also inhibited by inorganic phosphate.1 Publication

Kineticsi

  1. KM=115 µM for 4-nitrophenyl phosphate1 Publication
  2. KM=74 µM for 3'-AMP1 Publication
  3. KM=90 µM for 5'-AMP1 Publication
  4. KM=35 µM for ADP1 Publication
  5. KM=10 µM for ATP1 Publication

pH dependencei

Optimum pH is 8.5 with 4-nitrophenyl phosphate as substrate. Has 50% residual activity at pH 7.5 and 30% at pH 9.0, being virtually inactive at pH 10 and at pH 5 or lower.1 Publication

Temperature dependencei

Heat-labile. Incubation for 10 minutes at 50 or 60 degrees Celsius causes 40% and 60% inactivation, respectively. Heating at 100 degrees Celsius for 2 minutes causes total loss of activity. Heat-inactivated enzyme cannot be renaturated.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi38 – 381Zinc 1By similarity
Active sitei79 – 791Phosphothreonine intermediateBy similarity
Metal bindingi79 – 791Zinc 1By similarity
Binding sitei100 – 1001SubstrateBy similarity
Metal bindingi305 – 3051Zinc 2By similarity
Metal bindingi309 – 3091Zinc 2; via tele nitrogenBy similarity
Metal bindingi352 – 3521Zinc 1By similarity
Metal bindingi353 – 3531Zinc 1; via tele nitrogenBy similarity
Metal bindingi486 – 4861Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB
  2. carbohydrate phosphatase activity Source: UniProtKB
  3. glucose-1-phosphatase activity Source: UniProtKB
  4. glucose-6-phosphatase activity Source: UniProtKB
  5. metal ion binding Source: UniProtKB
  6. nucleotide phosphatase activity, acting on free nucleotides Source: UniProtKB
  7. sugar-phosphatase activity Source: UniProtKB
  8. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase PafAImported1 Publication (EC:3.1.3.11 Publication)
Short name:
AP PafA1 Publication
Gene namesi
Name:pafAImported
OrganismiElizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Taxonomic identifieri238 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeElizabethkingia

Subcellular locationi

Periplasm 1 Publication

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 546521Alkaline phosphatase PafASequence AnalysisPRO_0000425536Add
BLAST

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Expression is not repressed by inorganic phosphate.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9KJX5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni162 – 1643Substrate bindingBy similarity

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.720.10. 2 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMiSSF53649. SSF53649. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9KJX5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLTPKKWLLG VLVVSGMLGA QKTNAVPRPK LVVGLVVDQM RWDYLYRYYS
60 70 80 90 100
KYGEGGFKRM LNTGYSLNNV HIDYVPTVTA IGHTSIFTGS VPSIHGIAGN
110 120 130 140 150
DWYDKELGKS VYCTSDETVQ PVGTTSNSVG QHSPRNLWST TVTDQLGLAT
160 170 180 190 200
NFTSKVVGVS LKDRASILPA GHNPTGAFWF DDTTGKFITS TYYTKELPKW
210 220 230 240 250
VNDFNNKNVP AQLVANGWNT LLPINQYTES SEDNVEWEGL LGSKKTPTFP
260 270 280 290 300
YTDLAKDYEA KKGLIRTTPF GNTLTLQMAD AAIDGNQMGV DDITDFLTVN
310 320 330 340 350
LASTDYVGHN FGPNSIEVED TYLRLDRDLA DFFNNLDKKV GKGNYLVFLS
360 370 380 390 400
ADHGAAHSVG FMQAHKMPTG FFVEDMKKEM NAKLKQKFGA DNIIAAAMNY
410 420 430 440 450
QVYFDRKVLA DSKLELDDVR DYVMTELKKE PSVLYVLSTD EIWESSIPEP
460 470 480 490 500
IKSRVINGYN WKRSGDIQII SKDGYLSAYS KKGTTHSVWN SYDSHIPLLF
510 520 530 540
MGWGIKQGES NQPYHMTDIA PTVSSLLKIQ FPSGAVGKPI TEVIGR
Length:546
Mass (Da):60,753
Last modified:October 1, 2000 - v1
Checksum:i1A2CB1435BC74BD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157621 Genomic DNA. Translation: AAF80345.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157621 Genomic DNA. Translation: AAF80345.1 .

3D structure databases

ProteinModelPortali Q9KJX5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.720.10. 2 hits.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view ]
Pfami PF01663. Phosphodiest. 1 hit.
[Graphical view ]
PIRSFi PIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMi SSF53649. SSF53649. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "The Chryseobacterium meningosepticum PafA enzyme: prototype of a new enzyme family of prokaryotic phosphate-irrepressible alkaline phosphatases?"
    Berlutti F., Passariello C., Selan L., Thaller M.C., Rossolini G.M.
    Microbiology 147:2831-2839(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION.
    Strain: ATCC 13254 / CCUG 4310 / CIP 6058 / LMG 12280 / NCTC 10585Imported.

Entry informationi

Entry nameiALPH_ELIME
AccessioniPrimary (citable) accession number: Q9KJX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program