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Q9KJX5

- ALPH_ELIME

UniProt

Q9KJX5 - ALPH_ELIME

Protein

Alkaline phosphatase PafA

Gene

pafA

Organism
Elizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Alkaline phosphatase with broad substrate specificity. Has phosphatase activity towards nucleotide phosphates with a preference for ATP. Active towards a great variety of phosphomonoesters with the exception of 2',3'-cyclic AMP and myo-inositol hexakisphosphate.1 Publication

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

    Cofactori

    Binds 2 zinc ions.By similarity

    Enzyme regulationi

    Strongly inhibited by orthovanadate and EDTA. Also inhibited by inorganic phosphate.1 Publication

    Kineticsi

    1. KM=115 µM for 4-nitrophenyl phosphate1 Publication
    2. KM=74 µM for 3'-AMP1 Publication
    3. KM=90 µM for 5'-AMP1 Publication
    4. KM=35 µM for ADP1 Publication
    5. KM=10 µM for ATP1 Publication

    pH dependencei

    Optimum pH is 8.5 with 4-nitrophenyl phosphate as substrate. Has 50% residual activity at pH 7.5 and 30% at pH 9.0, being virtually inactive at pH 10 and at pH 5 or lower.1 Publication

    Temperature dependencei

    Heat-labile. Incubation for 10 minutes at 50 or 60 degrees Celsius causes 40% and 60% inactivation, respectively. Heating at 100 degrees Celsius for 2 minutes causes total loss of activity. Heat-inactivated enzyme cannot be renaturated.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi38 – 381Zinc 1By similarity
    Active sitei79 – 791Phosphothreonine intermediateBy similarity
    Metal bindingi79 – 791Zinc 1By similarity
    Binding sitei100 – 1001SubstrateBy similarity
    Metal bindingi305 – 3051Zinc 2By similarity
    Metal bindingi309 – 3091Zinc 2; via tele nitrogenBy similarity
    Metal bindingi352 – 3521Zinc 1By similarity
    Metal bindingi353 – 3531Zinc 1; via tele nitrogenBy similarity
    Metal bindingi486 – 4861Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB
    2. carbohydrate phosphatase activity Source: UniProtKB
    3. glucose-1-phosphatase activity Source: UniProtKB
    4. glucose-6-phosphatase activity Source: UniProtKB
    5. metal ion binding Source: UniProtKB
    6. nucleotide phosphatase activity, acting on free nucleotides Source: UniProtKB
    7. sugar-phosphatase activity Source: UniProtKB
    8. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase PafAImported1 Publication (EC:3.1.3.11 Publication)
    Short name:
    AP PafA1 Publication
    Gene namesi
    Name:pafAImported
    OrganismiElizabethkingia meningoseptica (Chryseobacterium meningosepticum)
    Taxonomic identifieri238 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeElizabethkingia

    Subcellular locationi

    Periplasm 1 Publication

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 546521Alkaline phosphatase PafASequence AnalysisPRO_0000425536Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Inductioni

    Expression is not repressed by inorganic phosphate.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9KJX5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni162 – 1643Substrate bindingBy similarity

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.720.10. 2 hits.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR026263. Alkaline_phosphatase_prok.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view]
    PfamiPF01663. Phosphodiest. 1 hit.
    [Graphical view]
    PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
    SUPFAMiSSF53649. SSF53649. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9KJX5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLTPKKWLLG VLVVSGMLGA QKTNAVPRPK LVVGLVVDQM RWDYLYRYYS    50
    KYGEGGFKRM LNTGYSLNNV HIDYVPTVTA IGHTSIFTGS VPSIHGIAGN 100
    DWYDKELGKS VYCTSDETVQ PVGTTSNSVG QHSPRNLWST TVTDQLGLAT 150
    NFTSKVVGVS LKDRASILPA GHNPTGAFWF DDTTGKFITS TYYTKELPKW 200
    VNDFNNKNVP AQLVANGWNT LLPINQYTES SEDNVEWEGL LGSKKTPTFP 250
    YTDLAKDYEA KKGLIRTTPF GNTLTLQMAD AAIDGNQMGV DDITDFLTVN 300
    LASTDYVGHN FGPNSIEVED TYLRLDRDLA DFFNNLDKKV GKGNYLVFLS 350
    ADHGAAHSVG FMQAHKMPTG FFVEDMKKEM NAKLKQKFGA DNIIAAAMNY 400
    QVYFDRKVLA DSKLELDDVR DYVMTELKKE PSVLYVLSTD EIWESSIPEP 450
    IKSRVINGYN WKRSGDIQII SKDGYLSAYS KKGTTHSVWN SYDSHIPLLF 500
    MGWGIKQGES NQPYHMTDIA PTVSSLLKIQ FPSGAVGKPI TEVIGR 546
    Length:546
    Mass (Da):60,753
    Last modified:October 1, 2000 - v1
    Checksum:i1A2CB1435BC74BD2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF157621 Genomic DNA. Translation: AAF80345.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF157621 Genomic DNA. Translation: AAF80345.1 .

    3D structure databases

    ProteinModelPortali Q9KJX5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.720.10. 2 hits.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR026263. Alkaline_phosphatase_prok.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view ]
    Pfami PF01663. Phosphodiest. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF031924. Pi-irrepressible_AP. 1 hit.
    SUPFAMi SSF53649. SSF53649. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The Chryseobacterium meningosepticum PafA enzyme: prototype of a new enzyme family of prokaryotic phosphate-irrepressible alkaline phosphatases?"
      Berlutti F., Passariello C., Selan L., Thaller M.C., Rossolini G.M.
      Microbiology 147:2831-2839(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION.
      Strain: ATCC 13254 / CCUG 4310 / CIP 6058 / LMG 12280 / NCTC 10585Imported.

    Entry informationi

    Entry nameiALPH_ELIME
    AccessioniPrimary (citable) accession number: Q9KJX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 19, 2014
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program