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Protein

Alkaline phosphatase PafA

Gene

pafA

Organism
Elizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Alkaline phosphatase with broad substrate specificity. Has phosphatase activity towards nucleotide phosphates with a preference for ATP. Active towards a great variety of phosphomonoesters with the exception of 2',3'-cyclic AMP and myo-inositol hexakisphosphate.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Enzyme regulationi

Strongly inhibited by orthovanadate and EDTA. Also inhibited by inorganic phosphate.1 Publication

Kineticsi

  1. KM=115 µM for 4-nitrophenyl phosphate1 Publication
  2. KM=74 µM for 3'-AMP1 Publication
  3. KM=90 µM for 5'-AMP1 Publication
  4. KM=35 µM for ADP1 Publication
  5. KM=10 µM for ATP1 Publication

    pH dependencei

    Optimum pH is 8.5 with 4-nitrophenyl phosphate as substrate. Has 50% residual activity at pH 7.5 and 30% at pH 9.0, being virtually inactive at pH 10 and at pH 5 or lower.1 Publication

    Temperature dependencei

    Heat-labile. Incubation for 10 minutes at 50 or 60 degrees Celsius causes 40% and 60% inactivation, respectively. Heating at 100 degrees Celsius for 2 minutes causes total loss of activity. Heat-inactivated enzyme cannot be renaturated.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi38Zinc 1By similarity1
    Active sitei79Phosphothreonine intermediateBy similarity1
    Metal bindingi79Zinc 1By similarity1
    Binding sitei100SubstrateBy similarity1
    Metal bindingi305Zinc 2By similarity1
    Metal bindingi309Zinc 2; via tele nitrogenBy similarity1
    Metal bindingi352Zinc 1By similarity1
    Metal bindingi353Zinc 1; via tele nitrogenBy similarity1
    Metal bindingi486Zinc 2; via tele nitrogenBy similarity1

    GO - Molecular functioni

    • alkaline phosphatase activity Source: UniProtKB
    • carbohydrate phosphatase activity Source: UniProtKB
    • glucose-1-phosphatase activity Source: UniProtKB
    • glucose-6-phosphatase activity Source: UniProtKB
    • metal ion binding Source: UniProtKB
    • nucleotide phosphatase activity, acting on free nucleotides Source: UniProtKB
    • sugar-phosphatase activity Source: UniProtKB
    • zinc ion binding Source: UniProtKB

    GO - Biological processi

    • dephosphorylation Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase
    LigandMetal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase PafA1 PublicationImported (EC:3.1.3.11 Publication)
    Short name:
    AP PafA1 Publication
    Gene namesi
    Name:pafAImported
    OrganismiElizabethkingia meningoseptica (Chryseobacterium meningosepticum)
    Taxonomic identifieri238 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeElizabethkingia

    Subcellular locationi

    • Periplasm 1 Publication

    GO - Cellular componenti

    • periplasmic space Source: UniProtKB

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 25Sequence analysisAdd BLAST25
    ChainiPRO_000042553626 – 546Alkaline phosphatase PafASequence analysisAdd BLAST521

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Inductioni

    Expression is not repressed by inorganic phosphate.1 Publication

    Structurei

    Secondary structure

    1546
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi30 – 37Combined sources8
    Helixi44 – 47Combined sources4
    Helixi49 – 51Combined sources3
    Beta strandi54 – 56Combined sources3
    Helixi57 – 63Combined sources7
    Beta strandi64 – 68Combined sources5
    Helixi79 – 88Combined sources10
    Helixi92 – 95Combined sources4
    Beta strandi99 – 104Combined sources6
    Turni105 – 108Combined sources4
    Beta strandi109 – 112Combined sources4
    Beta strandi121 – 123Combined sources3
    Helixi127 – 130Combined sources4
    Helixi142 – 149Combined sources8
    Turni150 – 152Combined sources3
    Beta strandi156 – 159Combined sources4
    Helixi163 – 170Combined sources8
    Beta strandi175 – 180Combined sources6
    Turni182 – 184Combined sources3
    Beta strandi187 – 190Combined sources4
    Turni191 – 193Combined sources3
    Helixi199 – 206Combined sources8
    Helixi209 – 213Combined sources5
    Turni214 – 216Combined sources3
    Helixi224 – 226Combined sources3
    Beta strandi245 – 247Combined sources3
    Helixi254 – 260Combined sources7
    Helixi262 – 267Combined sources6
    Helixi269 – 285Combined sources17
    Turni286 – 289Combined sources4
    Beta strandi290 – 293Combined sources4
    Beta strandi295 – 301Combined sources7
    Helixi303 – 311Combined sources9
    Helixi316 – 339Combined sources24
    Beta strandi344 – 350Combined sources7
    Helixi359 – 364Combined sources6
    Helixi376 – 388Combined sources13
    Beta strandi394 – 398Combined sources5
    Beta strandi401 – 404Combined sources4
    Helixi406 – 412Combined sources7
    Helixi416 – 428Combined sources13
    Beta strandi433 – 438Combined sources6
    Helixi451 – 458Combined sources8
    Turni461 – 463Combined sources3
    Beta strandi466 – 471Combined sources6
    Beta strandi481 – 483Combined sources3
    Beta strandi488 – 490Combined sources3
    Helixi491 – 494Combined sources4
    Beta strandi498 – 502Combined sources5
    Beta strandi507 – 510Combined sources4
    Helixi516 – 518Combined sources3
    Helixi519 – 527Combined sources9
    Turni541 – 543Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    5TJ3X-ray1.70A21-546[»]
    ProteinModelPortaliQ9KJX5.
    SMRiQ9KJX5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni162 – 164Substrate bindingBy similarity3

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.720.10. 2 hits.
    InterProiView protein in InterPro
    IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR026263. Alkaline_phosphatase_prok.
    IPR002591. Phosphodiest/P_Trfase.
    PfamiView protein in Pfam
    PF01663. Phosphodiest. 1 hit.
    PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
    SUPFAMiSSF53649. SSF53649. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9KJX5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLTPKKWLLG VLVVSGMLGA QKTNAVPRPK LVVGLVVDQM RWDYLYRYYS
    60 70 80 90 100
    KYGEGGFKRM LNTGYSLNNV HIDYVPTVTA IGHTSIFTGS VPSIHGIAGN
    110 120 130 140 150
    DWYDKELGKS VYCTSDETVQ PVGTTSNSVG QHSPRNLWST TVTDQLGLAT
    160 170 180 190 200
    NFTSKVVGVS LKDRASILPA GHNPTGAFWF DDTTGKFITS TYYTKELPKW
    210 220 230 240 250
    VNDFNNKNVP AQLVANGWNT LLPINQYTES SEDNVEWEGL LGSKKTPTFP
    260 270 280 290 300
    YTDLAKDYEA KKGLIRTTPF GNTLTLQMAD AAIDGNQMGV DDITDFLTVN
    310 320 330 340 350
    LASTDYVGHN FGPNSIEVED TYLRLDRDLA DFFNNLDKKV GKGNYLVFLS
    360 370 380 390 400
    ADHGAAHSVG FMQAHKMPTG FFVEDMKKEM NAKLKQKFGA DNIIAAAMNY
    410 420 430 440 450
    QVYFDRKVLA DSKLELDDVR DYVMTELKKE PSVLYVLSTD EIWESSIPEP
    460 470 480 490 500
    IKSRVINGYN WKRSGDIQII SKDGYLSAYS KKGTTHSVWN SYDSHIPLLF
    510 520 530 540
    MGWGIKQGES NQPYHMTDIA PTVSSLLKIQ FPSGAVGKPI TEVIGR
    Length:546
    Mass (Da):60,753
    Last modified:October 1, 2000 - v1
    Checksum:i1A2CB1435BC74BD2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF157621 Genomic DNA. Translation: AAF80345.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF157621 Genomic DNA. Translation: AAF80345.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    5TJ3X-ray1.70A21-546[»]
    ProteinModelPortaliQ9KJX5.
    SMRiQ9KJX5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Family and domain databases

    Gene3Di3.40.720.10. 2 hits.
    InterProiView protein in InterPro
    IPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR026263. Alkaline_phosphatase_prok.
    IPR002591. Phosphodiest/P_Trfase.
    PfamiView protein in Pfam
    PF01663. Phosphodiest. 1 hit.
    PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
    SUPFAMiSSF53649. SSF53649. 2 hits.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiALPH_ELIME
    AccessioniPrimary (citable) accession number: Q9KJX5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 19, 2014
    Last sequence update: October 1, 2000
    Last modified: February 15, 2017
    This is version 50 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.