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Protein

Alkaline phosphatase PafA

Gene

pafA

Organism
Elizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Alkaline phosphatase with broad substrate specificity. Has phosphatase activity towards nucleotide phosphates with a preference for ATP. Active towards a great variety of phosphomonoesters with the exception of 2',3'-cyclic AMP and myo-inositol hexakisphosphate.1 Publication

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Enzyme regulationi

Strongly inhibited by orthovanadate and EDTA. Also inhibited by inorganic phosphate.1 Publication

Kineticsi

  1. KM=115 µM for 4-nitrophenyl phosphate1 Publication
  2. KM=74 µM for 3'-AMP1 Publication
  3. KM=90 µM for 5'-AMP1 Publication
  4. KM=35 µM for ADP1 Publication
  5. KM=10 µM for ATP1 Publication

    pH dependencei

    Optimum pH is 8.5 with 4-nitrophenyl phosphate as substrate. Has 50% residual activity at pH 7.5 and 30% at pH 9.0, being virtually inactive at pH 10 and at pH 5 or lower.1 Publication

    Temperature dependencei

    Heat-labile. Incubation for 10 minutes at 50 or 60 degrees Celsius causes 40% and 60% inactivation, respectively. Heating at 100 degrees Celsius for 2 minutes causes total loss of activity. Heat-inactivated enzyme cannot be renaturated.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi38 – 381Zinc 1By similarity
    Active sitei79 – 791Phosphothreonine intermediateBy similarity
    Metal bindingi79 – 791Zinc 1By similarity
    Binding sitei100 – 1001SubstrateBy similarity
    Metal bindingi305 – 3051Zinc 2By similarity
    Metal bindingi309 – 3091Zinc 2; via tele nitrogenBy similarity
    Metal bindingi352 – 3521Zinc 1By similarity
    Metal bindingi353 – 3531Zinc 1; via tele nitrogenBy similarity
    Metal bindingi486 – 4861Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    • alkaline phosphatase activity Source: UniProtKB
    • carbohydrate phosphatase activity Source: UniProtKB
    • glucose-1-phosphatase activity Source: UniProtKB
    • glucose-6-phosphatase activity Source: UniProtKB
    • metal ion binding Source: UniProtKB
    • nucleotide phosphatase activity, acting on free nucleotides Source: UniProtKB
    • sugar-phosphatase activity Source: UniProtKB
    • zinc ion binding Source: UniProtKB

    GO - Biological processi

    • dephosphorylation Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase PafA1 PublicationImported (EC:3.1.3.11 Publication)
    Short name:
    AP PafA1 Publication
    Gene namesi
    Name:pafAImported
    OrganismiElizabethkingia meningoseptica (Chryseobacterium meningosepticum)
    Taxonomic identifieri238 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeElizabethkingia

    Subcellular locationi

    • Periplasm 1 Publication

    GO - Cellular componenti

    • periplasmic space Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 546521Alkaline phosphatase PafASequence AnalysisPRO_0000425536Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Inductioni

    Expression is not repressed by inorganic phosphate.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9KJX5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni162 – 1643Substrate bindingBy similarity

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.720.10. 2 hits.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR026263. Alkaline_phosphatase_prok.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view]
    PfamiPF01663. Phosphodiest. 1 hit.
    [Graphical view]
    PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
    SUPFAMiSSF53649. SSF53649. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9KJX5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLTPKKWLLG VLVVSGMLGA QKTNAVPRPK LVVGLVVDQM RWDYLYRYYS
    60 70 80 90 100
    KYGEGGFKRM LNTGYSLNNV HIDYVPTVTA IGHTSIFTGS VPSIHGIAGN
    110 120 130 140 150
    DWYDKELGKS VYCTSDETVQ PVGTTSNSVG QHSPRNLWST TVTDQLGLAT
    160 170 180 190 200
    NFTSKVVGVS LKDRASILPA GHNPTGAFWF DDTTGKFITS TYYTKELPKW
    210 220 230 240 250
    VNDFNNKNVP AQLVANGWNT LLPINQYTES SEDNVEWEGL LGSKKTPTFP
    260 270 280 290 300
    YTDLAKDYEA KKGLIRTTPF GNTLTLQMAD AAIDGNQMGV DDITDFLTVN
    310 320 330 340 350
    LASTDYVGHN FGPNSIEVED TYLRLDRDLA DFFNNLDKKV GKGNYLVFLS
    360 370 380 390 400
    ADHGAAHSVG FMQAHKMPTG FFVEDMKKEM NAKLKQKFGA DNIIAAAMNY
    410 420 430 440 450
    QVYFDRKVLA DSKLELDDVR DYVMTELKKE PSVLYVLSTD EIWESSIPEP
    460 470 480 490 500
    IKSRVINGYN WKRSGDIQII SKDGYLSAYS KKGTTHSVWN SYDSHIPLLF
    510 520 530 540
    MGWGIKQGES NQPYHMTDIA PTVSSLLKIQ FPSGAVGKPI TEVIGR
    Length:546
    Mass (Da):60,753
    Last modified:October 1, 2000 - v1
    Checksum:i1A2CB1435BC74BD2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF157621 Genomic DNA. Translation: AAF80345.1.
    RefSeqiWP_021348871.1. NZ_JNCG01000004.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF157621 Genomic DNA. Translation: AAF80345.1.
    RefSeqiWP_021348871.1. NZ_JNCG01000004.1.

    3D structure databases

    ProteinModelPortaliQ9KJX5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Family and domain databases

    Gene3Di3.40.720.10. 2 hits.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR017850. Alkaline_phosphatase_core.
    IPR026263. Alkaline_phosphatase_prok.
    IPR002591. Phosphodiest/P_Trfase.
    [Graphical view]
    PfamiPF01663. Phosphodiest. 1 hit.
    [Graphical view]
    PIRSFiPIRSF031924. Pi-irrepressible_AP. 1 hit.
    SUPFAMiSSF53649. SSF53649. 2 hits.
    ProtoNetiSearch...

    Publicationsi

    1. "The Chryseobacterium meningosepticum PafA enzyme: prototype of a new enzyme family of prokaryotic phosphate-irrepressible alkaline phosphatases?"
      Berlutti F., Passariello C., Selan L., Thaller M.C., Rossolini G.M.
      Microbiology 147:2831-2839(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION.
      Strain: ATCC 13254 / CCUG 4310 / CIP 6058 / LMG 12280 / NCTC 10585Imported.

    Entry informationi

    Entry nameiALPH_ELIME
    AccessioniPrimary (citable) accession number: Q9KJX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 19, 2014
    Last sequence update: October 1, 2000
    Last modified: March 4, 2015
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.