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Q9KJX5 (ALPH_ELIME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Alkaline phosphatase PafA

Short name=AP PafA
EC=3.1.3.1
Gene names
Name:pafA
OrganismElizabethkingia meningoseptica (Chryseobacterium meningosepticum)
Taxonomic identifier238 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeElizabethkingia

Protein attributes

Sequence length546 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Alkaline phosphatase with broad substrate specificity. Has phosphatase activity towards nucleotide phosphates with a preference for ATP. Active towards a great variety of phosphomonoesters with the exception of 2',3'-cyclic AMP and myo-inositol hexakisphosphate. Ref.1

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate. Ref.1

Cofactor

Binds 2 zinc ions By similarity.

Enzyme regulation

Strongly inhibited by orthovanadate and EDTA. Also inhibited by inorganic phosphate. Ref.1

Subcellular location

Periplasm Ref.1.

Induction

Expression is not repressed by inorganic phosphate. Ref.1

Biophysicochemical properties

Kinetic parameters:

KM=115 µM for 4-nitrophenyl phosphate Ref.1

KM=74 µM for 3'-AMP

KM=90 µM for 5'-AMP

KM=35 µM for ADP

KM=10 µM for ATP

pH dependence:

Optimum pH is 8.5 with 4-nitrophenyl phosphate as substrate. Has 50% residual activity at pH 7.5 and 30% at pH 9.0, being virtually inactive at pH 10 and at pH 5 or lower.

Temperature dependence:

Heat-labile. Incubation for 10 minutes at 50 or 60 degrees Celsius causes 40% and 60% inactivation, respectively. Heating at 100 degrees Celsius for 2 minutes causes total loss of activity. Heat-inactivated enzyme cannot be renaturated.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 546521Alkaline phosphatase PafA
PRO_0000425536

Regions

Region162 – 1643Substrate binding By similarity

Sites

Active site791Phosphothreonine intermediate By similarity
Metal binding381Zinc 1 By similarity
Metal binding791Zinc 1 By similarity
Metal binding3051Zinc 2 By similarity
Metal binding3091Zinc 2; via tele nitrogen By similarity
Metal binding3521Zinc 1 By similarity
Metal binding3531Zinc 1; via tele nitrogen By similarity
Metal binding4861Zinc 2; via tele nitrogen By similarity
Binding site1001Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KJX5 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 1A2CB1435BC74BD2

FASTA54660,753
        10         20         30         40         50         60 
MLTPKKWLLG VLVVSGMLGA QKTNAVPRPK LVVGLVVDQM RWDYLYRYYS KYGEGGFKRM 

        70         80         90        100        110        120 
LNTGYSLNNV HIDYVPTVTA IGHTSIFTGS VPSIHGIAGN DWYDKELGKS VYCTSDETVQ 

       130        140        150        160        170        180 
PVGTTSNSVG QHSPRNLWST TVTDQLGLAT NFTSKVVGVS LKDRASILPA GHNPTGAFWF 

       190        200        210        220        230        240 
DDTTGKFITS TYYTKELPKW VNDFNNKNVP AQLVANGWNT LLPINQYTES SEDNVEWEGL 

       250        260        270        280        290        300 
LGSKKTPTFP YTDLAKDYEA KKGLIRTTPF GNTLTLQMAD AAIDGNQMGV DDITDFLTVN 

       310        320        330        340        350        360 
LASTDYVGHN FGPNSIEVED TYLRLDRDLA DFFNNLDKKV GKGNYLVFLS ADHGAAHSVG 

       370        380        390        400        410        420 
FMQAHKMPTG FFVEDMKKEM NAKLKQKFGA DNIIAAAMNY QVYFDRKVLA DSKLELDDVR 

       430        440        450        460        470        480 
DYVMTELKKE PSVLYVLSTD EIWESSIPEP IKSRVINGYN WKRSGDIQII SKDGYLSAYS 

       490        500        510        520        530        540 
KKGTTHSVWN SYDSHIPLLF MGWGIKQGES NQPYHMTDIA PTVSSLLKIQ FPSGAVGKPI 


TEVIGR 

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References

[1]"The Chryseobacterium meningosepticum PafA enzyme: prototype of a new enzyme family of prokaryotic phosphate-irrepressible alkaline phosphatases?"
Berlutti F., Passariello C., Selan L., Thaller M.C., Rossolini G.M.
Microbiology 147:2831-2839(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE NAME, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION.
Strain: ATCC 13254 / CCUG 4310 / CIP 6058 / LMG 12280 / NCTC 10585.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF157621 Genomic DNA. Translation: AAF80345.1.

3D structure databases

ProteinModelPortalQ9KJX5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.720.10. 2 hits.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR026263. Alkaline_phosphatase_prok.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PfamPF01663. Phosphodiest. 1 hit.
[Graphical view]
PIRSFPIRSF031924. Pi-irrepressible_AP. 1 hit.
SUPFAMSSF53649. SSF53649. 2 hits.
ProtoNetSearch...

Entry information

Entry nameALPH_ELIME
AccessionPrimary (citable) accession number: Q9KJX5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program