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Protein

Signal transduction histidine-protein kinase ArlS

Gene

arlS

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system ArlS/ArlR involved in the regulation of adhesion, autolysis, multidrug resistance and virulence. ArlS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to ArlR. ArlS/ArlR affects expression of the multidrug resistance transporter norA and interacts with both Agr (virulence accessory gene regulator) (negatively) and SarA (staphylococcal accessory regulator) (positively) to modulate several virulence factor genes, including ssp (serine protease), spa (surface protein A) and hla (alpha-hemolysin). Could inhibit biofilm development by a mechanism independent of the presence of the poly-N-acetylglucosamine (PNAG). Also, Arl proteins are required for the efficient activity of DNA gyrase inhibitors and high osmolarity on spa expression.5 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: InterPro
  • serine-type peptidase activity Source: CACAO

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system, Virulence
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR93061:G1G5Y-1324-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transduction histidine-protein kinase ArlS (EC:2.7.13.3)
Gene namesi
Name:arlS
Ordered Locus Names:SAOUHSC_01419
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31HelicalSequence analysisAdd BLAST21
Transmembranei156 – 176HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000746881 – 451Signal transduction histidine-protein kinase ArlSAdd BLAST451

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei242Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.Curated

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Activated by agr, SarA, SarV and MgrA.

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01419

Structurei

3D structure databases

ProteinModelPortaliQ9KJN3
SMRiQ9KJN3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 231HAMPPROSITE-ProRule annotationAdd BLAST54
Domaini239 – 451Histidine kinasePROSITE-ProRule annotationAdd BLAST213

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105GR3 Bacteria
COG0642 LUCA
HOGENOMiHOG000223177
KOiK18940
OMAiMTIMIND

Family and domain databases

CDDicd06225 HAMP, 1 hit
cd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
Gene3Di3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR003660 HAMP_dom
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR004358 Sig_transdc_His_kin-like_C
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SUPFAMiSSF47384 SSF47384, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS50885 HAMP, 1 hit
PS50109 HIS_KIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q9KJN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKRKLRNNW IIVTTMITFV TIFLFCLIII FFLKDTLHNS ELDDAERSSS
60 70 80 90 100
DINNLFHSKP VKDISALDLN ASLGNFQEII IYDEHNNKLF ETSNDNTVRV
110 120 130 140 150
EPGYEHRYFD RVIKKRYKGI EYLIIKEPIT TQDFKGYSLL IHSLENYDNI
160 170 180 190 200
VKSLYIIALA FGVIATIITA TISYVFSTQI TKPLVSLSNK MIEIRRDGFQ
210 220 230 240 250
NKLQLNTNYE EIDNLANTFN EMMSQIEESF NQQRQFVEDA SHELRTPLQI
260 270 280 290 300
IQGHLNLIQR WGKKDPAVLE ESLNISIEEM NRIIKLVEEL LELTKGDVND
310 320 330 340 350
ISSEAQTVHI NDEIRSRIHS LKQLHPDYQF DTDLTSKNLE IKMKPHQFEQ
360 370 380 390 400
LFLIFIDNAI KYDVKNKKIK VKTRLKNKQK IIEITDHGIG IPEEDQDFIF
410 420 430 440 450
DRFYRVDKSR SRSQGGNGLG LSIAQKIIQL NGGSIKIKSE INKGTTFKII

F
Length:451
Mass (Da):52,400
Last modified:October 1, 2000 - v1
Checksum:i6308576067A22438
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165314 Genomic DNA Translation: AAF85897.1
CP000253 Genomic DNA Translation: ABD30512.1
RefSeqiWP_000166801.1, NC_007795.1
YP_499945.1, NC_007795.1

Genome annotation databases

EnsemblBacteriaiABD30512; ABD30512; SAOUHSC_01419
GeneIDi3920650
KEGGisao:SAOUHSC_01419
PATRICifig|93061.5.peg.1298

Similar proteinsi

Entry informationi

Entry nameiARLS_STAA8
AccessioniPrimary (citable) accession number: Q9KJN3
Secondary accession number(s): Q2FYM0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: October 1, 2000
Last modified: February 28, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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