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Protein

Signal transduction histidine-protein kinase ArlS

Gene

arlS

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system ArlS/ArlR involved in the regulation of adhesion, autolysis, multidrug resistance and virulence. ArlS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to ArlR. ArlS/ArlR affects expression of the multidrug resistance transporter norA and interacts with both Agr (virulence accessory gene regulator) (negatively) and SarA (staphylococcal accessory regulator) (positively) to modulate several virulence factor genes, including ssp (serine protease), spa (surface protein A) and hla (alpha-hemolysin). Could inhibit biofilm development by a mechanism independent of the presence of the poly-N-acetylglucosamine (PNAG). Also, Arl proteins are required for the efficient activity of DNA gyrase inhibitors and high osmolarity on spa expression.5 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: InterPro
  • serine-type peptidase activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system, Virulence

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-1359-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transduction histidine-protein kinase ArlS (EC:2.7.13.3)
Gene namesi
Name:arlS
Ordered Locus Names:SAOUHSC_01419
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121HelicalSequence analysisAdd
BLAST
Transmembranei156 – 17621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451Signal transduction histidine-protein kinase ArlSPRO_0000074688Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei242 – 2421Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.Curated

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Activated by agr, SarA, SarV and MgrA.

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01419.

Structurei

3D structure databases

ProteinModelPortaliQ9KJN3.
SMRiQ9KJN3. Positions 180-449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini178 – 23154HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini239 – 451213Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105GR3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000223177.
KOiK18940.
OMAiASSINQM.
OrthoDBiEOG6G4VQG.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KJN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKRKLRNNW IIVTTMITFV TIFLFCLIII FFLKDTLHNS ELDDAERSSS
60 70 80 90 100
DINNLFHSKP VKDISALDLN ASLGNFQEII IYDEHNNKLF ETSNDNTVRV
110 120 130 140 150
EPGYEHRYFD RVIKKRYKGI EYLIIKEPIT TQDFKGYSLL IHSLENYDNI
160 170 180 190 200
VKSLYIIALA FGVIATIITA TISYVFSTQI TKPLVSLSNK MIEIRRDGFQ
210 220 230 240 250
NKLQLNTNYE EIDNLANTFN EMMSQIEESF NQQRQFVEDA SHELRTPLQI
260 270 280 290 300
IQGHLNLIQR WGKKDPAVLE ESLNISIEEM NRIIKLVEEL LELTKGDVND
310 320 330 340 350
ISSEAQTVHI NDEIRSRIHS LKQLHPDYQF DTDLTSKNLE IKMKPHQFEQ
360 370 380 390 400
LFLIFIDNAI KYDVKNKKIK VKTRLKNKQK IIEITDHGIG IPEEDQDFIF
410 420 430 440 450
DRFYRVDKSR SRSQGGNGLG LSIAQKIIQL NGGSIKIKSE INKGTTFKII

F
Length:451
Mass (Da):52,400
Last modified:October 1, 2000 - v1
Checksum:i6308576067A22438
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165314 Genomic DNA. Translation: AAF85897.1.
CP000253 Genomic DNA. Translation: ABD30512.1.
RefSeqiYP_499945.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD30512; ABD30512; SAOUHSC_01419.
GeneIDi3920650.
KEGGisao:SAOUHSC_01419.
PATRICi19580277. VBIStaAur99865_1298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165314 Genomic DNA. Translation: AAF85897.1.
CP000253 Genomic DNA. Translation: ABD30512.1.
RefSeqiYP_499945.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ9KJN3.
SMRiQ9KJN3. Positions 180-449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01419.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD30512; ABD30512; SAOUHSC_01419.
GeneIDi3920650.
KEGGisao:SAOUHSC_01419.
PATRICi19580277. VBIStaAur99865_1298.

Phylogenomic databases

eggNOGiENOG4105GR3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000223177.
KOiK18940.
OMAiASSINQM.
OrthoDBiEOG6G4VQG.

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-1359-MONOMER.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new two-component regulatory system involved in adhesion, autolysis, and extracellular proteolytic activity of Staphylococcus aureus."
    Fournier B., Hooper D.C.
    J. Bacteriol. 182:3955-3964(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The Staphylococcus aureus NCTC 8325 genome."
    Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
    (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 8325.
  3. "Expression of the multidrug resistance transporter NorA from Staphylococcus aureus is modified by a two-component regulatory system."
    Fournier B., Aras R., Hooper D.C.
    J. Bacteriol. 182:664-671(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "The two-component system ArlS-ArlR is a regulator of virulence gene expression in Staphylococcus aureus."
    Fournier B., Klier A., Rapoport G.
    Mol. Microbiol. 41:247-261(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGULATION.
  5. "Characterization of RAT, an autolysis regulator in Staphylococcus aureus."
    Ingavale S.S., Van Wamel W., Cheung A.L.
    Mol. Microbiol. 48:1451-1466(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION BY MGRA.
  6. "Identification of sarV (SA2062), a new transcriptional regulator, is repressed by SarA and MgrA (SA0641) and involved in the regulation of autolysis in Staphylococcus aureus."
    Manna A.C., Ingavale S.S., Maloney M., van Wamel W., Cheung A.L.
    J. Bacteriol. 186:5267-5280(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION BY SARV.
  7. "Protein A gene expression is regulated by DNA supercoiling which is modified by the ArlS-ArlR two-component system of Staphylococcus aureus."
    Fournier B., Klier A.
    Microbiology 150:3807-3819(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system."
    Toledo-Arana A., Merino N., Vergara-Irigaray M., Debarbouille M., Penades J.R., Lasa I.
    J. Bacteriol. 187:5318-5329(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN BIOFILM DEVELOPMENT.

Entry informationi

Entry nameiARLS_STAA8
AccessioniPrimary (citable) accession number: Q9KJN3
Secondary accession number(s): Q2FYM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.