Q9KJG4 (RPOB_BARHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	BH06100

Organism

Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

283166 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	1383 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1383	1383	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047862

Sequences

	Sequence		Length	Mass (Da)
	Q9KJG4 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2CEE87E06A3207B6	FASTA	1,383	154,853
	10 20 30 40 50 60 MAQTLAMTSQ FNGRKRVRKF FGKIPEVAEM PNLIEVQKAS YDQFLMIEEP KGGRPDEGLQ 70 80 90 100 110 120 AVFKSVFPIS DFSGTAMLEF VGYQFDSPKF DVEECRQRDL TYAAPLKVIL RLIVFDVDED 130 140 150 160 170 180 TGSKDIKDIK EQGVYMGDMP LMTTNGTFIV NGTERVIVSQ MHRSPGVFFD HDKGKSHSSG 190 200 210 220 230 240 KFLFAARVIP YRGSWLDIEF DAKDIIYARI DRRRKIPVTS LLMALGMDAS DILSTFYNKV 250 260 270 280 290 300 TYERDGDGWR IPYSVDRFKG MKLVSDLIDA DSGEVVAEAG KKLTVRAAKA LAEKGLKAVK 310 320 330 340 350 360 VSEDDLLGSY LAEDIVNYQT GEIYLEAGDE IDEKTLRVLF DVNADQIDIL DIDHMNIGAY 370 380 390 400 410 420 IRNTLKVDKN ESRQDALFDI YRVMRPGEPP TMDTAEAMFH SLFFDPERYD LSAVGRVKMN 430 440 450 460 470 480 LRMDLDCPDT VRVLRQEDIL AVVKMLVELR DGRGEIDDID NLGNRRVRSV GELMENQYRI 490 500 510 520 530 540 GLLRMERAIK ERMSSVEIDT VMPQDLINAK PAAAAVREFF GSSQLSQFMD QTNPLSEITH 550 560 570 580 590 600 KRRLSALGPG GLTRERAGFE VRDVHPTHYG RICPIETPEG PNIGLINSLA TFARVNKYGF 610 620 630 640 650 660 IESPYRKIID GKVTTEVIYL SAMEESKHYV AQANSSLDAE GRLSEEFVVC RHAGEVLMAP 670 680 690 700 710 720 RDHVDLMDVS PKQLVSVAAA LIPFLENDDA NRALMGSNMQ RQAVPLVRAE APFVGTGMES 730 740 750 760 770 780 IVARDSGAAV AARRSGIVDQ VDATRIVIRA TEDLDPSKSG VDIYRLQKFQ RSNQSTCINQ 790 800 810 820 830 840 RPLVHVGDRV EKGNIIADGP STDLGDLALG RNVLVAFMPW NGYNYEDSIL LSERIVADDV 850 860 870 880 890 900 FTSIHIEEFE VAARDTKLGP EEITRDIPNV AEEALRNLDE AGIIYIGAEV QPGDILVGKI 910 920 930 940 950 960 TPKGESPMTP EEKLLRAIFG EKASDVRDTS MRMPPGAFGT VVEVRVFNRH GVEKDERAMA 970 980 990 1000 1010 1020 IEREEIERLA KDRDDEQSIL DRNVYARLTD MLVGKVAVEG PKGFSKNKKL DTTIMGHYPR 1030 1040 1050 1060 1070 1080 SQWWQFTVED EKLQNEIEAL RNQYDESKEA LQRRFMDKVE KVQRGDEMPP GVMKMVKVFV 1090 1100 1110 1120 1130 1140 AVKRKIQPGD KMAGRHGNKG VVSRILPIED MPFLEDGTHA DIVLNPLGVP SRMNVGQILE 1150 1160 1170 1180 1190 1200 THLGWACAGM GKKIGDLVDL YQETGDILPL RQRIENLMPD DDHNEPVRQY DNESLYKLAL 1210 1220 1230 1240 1250 1260 QMRKGVSIAT PVFDGAHEAD INMMLEDAGL DSSGQVTLYD GRTGEPFDRP VTVGYIYMLK 1270 1280 1290 1300 1310 1320 LHHLVDDKIH ARSIGPYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA YTLQEMLTVK 1330 1340 1350 1360 1370 1380 SDDVAGRTKV YEAIVRGDDT FEAGIPESFN VLVKEMRSLA LNVELDDARE LIAQRALSDT TEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Use of rpoB gene analysis for detection and identification of Bartonella species." Renesto P., Gouvernet J., Drancourt M., Roux V., Raoult D. J. Clin. Microbiol. 39:430-437(2001) [PubMed: 11158086] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.
[2]	"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF171070 Genomic DNA. Translation: AAF87049.1. BX897699 Genomic DNA. Translation: CAF27414.1.
RefSeq	YP_033439.1. NC_005956.1.
3D structure databases
ProteinModelPortal	Q9KJG4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2864852.
GenomeReviews	Gene locus BH06100 in contig BX897699_GR.
KEGG	bhe:BH06100.
NMPDR	fig|283166.1.peg.567.
PATRIC	20545035. VBIBarHen29080_0666.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285547.
OMA	QRDLTYA.
PhylomeDB	Q9KJG4.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	BHEN283166:BH06100-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_BARHE

Accession

Primary (citable) accession number: Q9KJG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program