Reviewed, UniProtKB/Swiss-Prot Q9KJE8 (BBSG_THAAR)

Names and origin

Protein names

Recommended name:
    (R)-benzylsuccinyl-CoA dehydrogenase
    EC=1.3.99.21

Gene names

Name:

bbsG

Organism

Thauera aromatica

Taxonomic identifier

59405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Thauera

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(R)-2-benzylsuccinyl-CoA + 2 electron-transferring flavoprotein = (E)-2-benzylidenesuccinyl-CoA + 2 reduced electron-transferring flavoprotein.
Cofactor	FAD.
Enzyme regulation	Inhibited by (S)-benzylsuccinyl-CoA.
Pathway	Xenobiotic degradation; toluene degradation.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the acyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	(R)-benzylsuccinyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC FAD binding Inferred from electronic annotation. Source: InterPro acyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	(R)-benzylsuccinyl-CoA dehydrogenase		PRO_0000201205

Sequences

	Sequence		Length	Mass (Da)
	Q9KJE8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: FC6B7DDC3BD48B11	FASTA	406	45,583
	10 20 30 40 50 60 MDFSLSEEQT MLKEVARRFT ANELMPLEKV LLEREMRMWT DGYTLLPEAD HARLMKITQE 70 80 90 100 110 120 MGFWGIEVDE KLGGQGLGMF AKTLVVEEMS KSLIGFSHHG FTLPPDAPNL YYLEECGSPA 130 140 150 160 170 180 QRDKYVRRYC RGEIDSAMMA TEPGAGSDIS GLTTTAVREN GQWVINGSKI FISKCDKDEL 190 200 210 220 230 240 FFICIAVTDK EAPTKRRFTA FILDKDTPGL RIGAEIPVIG AMPTWSVYLD NVRVGDEAVL 250 260 270 280 290 300 GEVGDAFIPL QNRFGVRRIE LAAHCTGMAE RLIQMMIDQA NLRKTFGVAL ADRQTVQNWI 310 320 330 340 350 360 ADSTIELEQV RLQLYFTAWK SDQGHKDLRL EAASLKIAAT EMLTRVADRA IQLHGGLGLS 370 380 390 400 REMGIEYVAR MVRIWRVVEG ASEIHRMSIA KKLLTDGRTY SPFVAA

References

[1]	"Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta-oxidation of the intermediate benzylsuccinate." Leuthner B., Heider J. J. Bacteriol. 182:272-277(2000) [PubMed: 10629170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 6984 / K172.
[2]	"(R)-benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway." Leutwein C., Heider J. Arch. Microbiol. 178:517-524(2002) [PubMed: 12420174] [Abstract] Cited for: CHARACTERIZATION. Strain: DSM 6984 / K172.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF173961 Genomic DNA. Translation: AAF89842.1.
3D structure databases
HSSP	HSSP built from PDB template 1JQI based on UniProtKB P15651.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-687.
BRENDA	1.3.99.21. 377.
Family and domain databases
InterPro	IPR006089. Acyl-CoA_DH_CS. IPR006092. Acyl-CoA_DH_N. IPR006090. Acyl-CoA_Oxase/DH_1. IPR006091. Acyl-CoA_Oxase/DH_M. IPR013786. AcylCoA_DH/ox_N. IPR013764. AcylCoA_oxidase/DH_1/2_C. [Graphical view]
Gene3D	G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit. G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit. G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit.
Pfam	PF00441. Acyl-CoA_dh_1. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. PF02771. Acyl-CoA_dh_N. 1 hit. [Graphical view]
PROSITE	PS00072. ACYL_COA_DH_1. False negative. PS00073. ACYL_COA_DH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BBSG_THAAR

Accession

Primary (citable) accession number: Q9KJE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)