Reviewed, UniProtKB/Swiss-Prot Q9KJB0 (BLAB6_FLAME)

Names and origin

Protein names

Recommended name:
    Carbapenem-hydrolyzing beta-lactamase blaB-6
      Short name=CHbetaL-6
    EC=3.5.2.6
Alternative name(s):
    Class B carbapenemase blaB-6

Gene names

Name:	blaB6
Synonyms:	blaB

Organism

Flavobacterium meningosepticum (Chryseobacterium meningosepticum) (Elizabethkingia meningoseptica)

Taxonomic identifier

238 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteria › Flavobacteriales › Flavobacteriaceae › Elizabethkingia

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes penicillins, cephalosporins (including cefoxitin), carbapenems and 6-beta-iodopenicillanate By similarity.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the class-B beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	By similarity
	Chain	22 – 248	227	Carbapenem-hydrolyzing beta-lactamase blaB-6		PRO_0000016954
Sites
	Metal binding	97	1	Zinc 1 By similarity
	Metal binding	99	1	Zinc 1 By similarity
	Metal binding	101	1	Zinc 2 By similarity
	Metal binding	160	1	Zinc 1 By similarity
	Metal binding	179	1	Zinc 2 By similarity
	Metal binding	221	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KJB0-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 720CF80F4A1EA837	FASTA	248	28,046
	10 20 30 40 50 60 MKRLKGLLVL ALGFTGLQVF GQQNPDIKIE KLKDNLYVYT TYNTFKGTKY AANAVYMVTD 70 80 90 100 110 120 KGVVVIDSPW GEDKFKSFTD EIYKKHGKKV IMNIATHSHD DRAGGLEYFG KLGAKTYSTK 130 140 150 160 170 180 MTDSILAKEN KPRAKYTFDN NKSFKVGNTE FQVYYPGKGH TADNVVVWFP KDKVLVGGCI 190 200 210 220 230 240 VKSGDSKDLG FIGEAYVNDW TQSIHNIQQK FPDVQYVVAG HDDWKDQTSI QHTLDLISEY QQKQKASN

References

[1]

"Molecular and biochemical heterogeneity of class B carbapenem-hydrolyzing beta-lactamases in Chryseobacterium meningosepticum." Bellais S., Aubert D., Naas T., Nordmann P. Antimicrob. Agents Chemother. 44:1878-1886(2000) [PubMed: 10858348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCTC 11305 / CIP 78.30.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF189302 Genomic DNA. Translation: AAF89158.1.
3D structure databases
HSSP	HSSP built from PDB template 2BC2 based on UniProtKB P04190.
SMR	Q9KJB0. Positions 26-244.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 39124.
Family and domain databases
InterPro	IPR001018. Beta-lactamase_class-B_CS. IPR001279. Blactmase-like. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
PROSITE	PS00743. BETA_LACTAMASE_B_1. 1 hit. PS00744. BETA_LACTAMASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAB6_FLAME

Accession

Primary (citable) accession number: Q9KJB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)