Reviewed, UniProtKB/Swiss-Prot Q9KJA9 (BLAB5_FLAME)

Names and origin

Protein names

Recommended name:
    Carbapenem-hydrolyzing beta-lactamase blaB-5
      Short name=CHbetaL-5
    EC=3.5.2.6
Alternative name(s):
    Class B carbapenemase blaB-5

Gene names

Name:	blaB5
Synonyms:	blaB

Organism

Flavobacterium meningosepticum (Chryseobacterium meningosepticum) (Elizabethkingia meningoseptica)

Taxonomic identifier

238 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteria › Flavobacteriales › Flavobacteriaceae › Elizabethkingia

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes penicillins, cephalosporins (including cefoxitin), carbapenems and 6-beta-iodopenicillanate By similarity.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the class-B beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 249	227	Carbapenem-hydrolyzing beta-lactamase blaB-5		PRO_0000016953
Sites
	Metal binding	98	1	Zinc 1 By similarity
	Metal binding	100	1	Zinc 1 By similarity
	Metal binding	102	1	Zinc 2 By similarity
	Metal binding	161	1	Zinc 1 By similarity
	Metal binding	180	1	Zinc 2 By similarity
	Metal binding	222	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KJA9-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 252BA20FCEF1A837	FASTA	249	28,161
	10 20 30 40 50 60 MLKRLKGLLV LALGFTGLQV FGQQNPDIKI EKLKDNLYVY TTYNTFKGTK YAANAVYMVT 70 80 90 100 110 120 DKGVVVIDSP WGEDKFKSFT DEIYKKHGKK VIMNIATHSH DDRAGGLEYF GKLGAKTYST 130 140 150 160 170 180 KMTDSILAKE NKPRAKYTFD NNKSFKVGNT EFQVYYPGKG HTADNVVVWF PKDKVLVGGC 190 200 210 220 230 240 IVKSGDSKDL GYIGEAYVND WTQSIHNIQQ KFPDVQYVVA GHDDWKDQTS IQHTLDLISD YQQKQKASN

References

[1]

"Molecular and biochemical heterogeneity of class B carbapenem-hydrolyzing beta-lactamases in Chryseobacterium meningosepticum." Bellais S., Aubert D., Naas T., Nordmann P. Antimicrob. Agents Chemother. 44:1878-1886(2000) [PubMed: 10858348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCTC 11306 / CIP 79.5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF189303 Genomic DNA. Translation: AAF89159.1.
3D structure databases
HSSP	HSSP built from PDB template 2BC2 based on UniProtKB P04190.
SMR	Q9KJA9. Positions 27-245.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 39124.
Family and domain databases
InterPro	IPR001018. Beta-lactamase_class-B_CS. IPR001279. Blactmase-like. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
PROSITE	PS00743. BETA_LACTAMASE_B_1. 1 hit. PS00744. BETA_LACTAMASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAB5_FLAME

Accession

Primary (citable) accession number: Q9KJA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)