Q9KJ22 (GSMT_ECTHL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glycine/sarcosine N-methyltransferase EC=2.1.1.156
Organism	Ectothiorhodospira halochloris
Taxonomic identifier	1052 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Halorhodospira

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the methylation of glycine and sarcosine to sarcosine and dimethylglycine, respectively, with S-adenosylmethionine (AdoMet) acting as the methyl donor. It has strict specificity for glycine and sarcosine as the methyl group acceptors. Ref.2
Catalytic activity	S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine. Ref.2 2 S-adenosyl-L-methionine + glycine = 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine. Ref.2
Enzyme regulation	p-chloromercuribenzoic acid inhibits more than 95% of the GSMT activities on glycine and sarcosine, and S-adenosylhomocysteine (AdoHcy) inhibits completely GSMT activities. Ref.2
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via glycine pathway; betaine from glycine: step 1/3. Amine and polyamine biosynthesis; betaine biosynthesis via glycine pathway; betaine from glycine: step 2/3.
Subunit structure	Monomer. Ref.2
Sequence similarities	Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.
Biophysicochemical properties	Kinetic parameters: KM=2.3 mM for sarcosine (at pH 7.4 and at 37 degrees Celsius) Ref.2 KM=18 mM for glycine (at pH 7.4 and at 37 degrees Celsius) KM=0.28 mM for AdoMet (with sarcosine at pH 7.4 and at 37 degrees Celsius) KM=0.42 mM for AdoMet (with glycine at pH 7.4 and at 37 degrees Celsius) Vmax=0.12 µmol/min/mg enzyme with AdoMet as substrate (with sarcosine at pH 7.4 and at 37 degrees Celsius) Vmax=0.15 µmol/min/mg enzyme with sarcosine as substrate (at pH 7.4 and at 37 degrees) Vmax=1.0 µmol/min/mg enzyme with AdoMet as substrate (with glycine at pH 7.4 and at 37 degrees Celsius) Vmax=1.1 µmol/min/mg enzyme with glycine as substrate (at pH 7.4 and at 37 degrees) pH dependence: Optimum pH is around 7.4 and 7.9 for glycine and sarcosine, respectively.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	glycine betaine biosynthetic process from glycine Inferred from direct assay Ref.2. Source: UniProtKB
Molecular_function	glycine N-methyltransferase activity Inferred from electronic annotation. Source: EC sarcosine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Glycine/sarcosine N-methyltransferase		PRO_0000413611
Regions
	Region	114 – 115	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	26	1	S-adenosyl-L-methionine By similarity
	Binding site	34	1	S-adenosyl-L-methionine By similarity
	Binding site	43	1	S-adenosyl-L-methionine By similarity
	Binding site	67	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	88	1	S-adenosyl-L-methionine By similarity
	Binding site	132	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	206	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KJ22 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 6FEF20EB9DA52186	FASTA	268	31,184
	10 20 30 40 50 60 MNTTTEQDFG ADPTKVRDTD HYTEEYVDGF VDKWDDLIDW DSRAKSEGDF FIQELKKRGA 70 80 90 100 110 120 TRILDAATGT GFHSVRLLEA GFDVVSADGS AEMLAKAFEN GRKRGHILRT VQVDWRWLNR 130 140 150 160 170 180 DIHGRYDAII CLGNSFTHLF NEKDRRKTLA EFYSALNPEG VLILDQRNYD GILDHGYDSS 190 200 210 220 230 240 HSYYYCGEGV SVYPEHVDDG LARFKYEFND GSTYFLNMFP LRKDYTRRLM HEVGFQKIDT 250 260 YGDFKATYRD ADPDFFIHVA EKEYREED

References

[1]

"Extreme halophiles synthesize betaine from glycine by methylation." Nyyssola A., Kerovuo J., Kaukinen P., von Weymarn N., Reinikainen T. J. Biol. Chem. 275:22196-22201(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

[2]

"Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase." Nyyssola A., Reinikainen T., Leisola M. Appl. Environ. Microbiol. 67:2044-2050(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A METHYLTRANSFERASE.FUNCTION IN THE BETAINE BIOSYNTHESIS, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF216281 Genomic DNA. Translation: AAF87202.1.
3D structure databases
ProteinModelPortal	Q9KJ22.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-8542.
BRENDA	2.1.1.156. 97308.
UniPathway	UPA00530; UER00381. UPA00530; UER00382.
Family and domain databases
InterPro	IPR014369. Gly/Sar_N_MeTrfase. [Graphical view]
PANTHER	PTHR16458. PTHR16458. 1 hit.
PIRSF	PIRSF000385. Gly_N-mtase. 1 hit.
PROSITE	PS51600. SAM_GNMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSMT_ECTHL

Accession

Primary (citable) accession number: Q9KJ22

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	October 1, 2000
Last modified:	May 29, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program