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Protein

Glycine/sarcosine N-methyltransferase

Gene
N/A
Organism
Halorhodospira halochloris (Ectothiorhodospira halochloris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylation of glycine and sarcosine to sarcosine and dimethylglycine, respectively, with S-adenosylmethionine (AdoMet) acting as the methyl donor. It has strict specificity for glycine and sarcosine as the methyl group acceptors.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.1 Publication
2 S-adenosyl-L-methionine + glycine = 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine.1 Publication

Enzyme regulationi

p-chloromercuribenzoic acid inhibits more than 95% of the GSMT activities on glycine and sarcosine, and S-adenosylhomocysteine (AdoHcy) inhibits completely GSMT activities.1 Publication

Kineticsi

  1. KM=2.3 mM for sarcosine (at pH 7.4 and at 37 degrees Celsius)1 Publication
  2. KM=18 mM for glycine (at pH 7.4 and at 37 degrees Celsius)1 Publication
  3. KM=0.28 mM for AdoMet (with sarcosine at pH 7.4 and at 37 degrees Celsius)1 Publication
  4. KM=0.42 mM for AdoMet (with glycine at pH 7.4 and at 37 degrees Celsius)1 Publication
  1. Vmax=0.12 µmol/min/mg enzyme with AdoMet as substrate (with sarcosine at pH 7.4 and at 37 degrees Celsius)1 Publication
  2. Vmax=0.15 µmol/min/mg enzyme with sarcosine as substrate (at pH 7.4 and at 37 degrees)1 Publication
  3. Vmax=1.0 µmol/min/mg enzyme with AdoMet as substrate (with glycine at pH 7.4 and at 37 degrees Celsius)1 Publication
  4. Vmax=1.1 µmol/min/mg enzyme with glycine as substrate (at pH 7.4 and at 37 degrees)1 Publication

pH dependencei

Optimum pH is around 7.4 and 7.9 for glycine and sarcosine, respectively.1 Publication

Pathwayi: betaine biosynthesis via glycine pathway

This protein is involved in step 1 and 2 of the subpathway that synthesizes betaine from glycine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycine/sarcosine N-methyltransferase
  2. Sarcosine/dimethylglycine N-methyltransferase, Glycine/sarcosine N-methyltransferase
  3. Sarcosine/dimethylglycine N-methyltransferase
This subpathway is part of the pathway betaine biosynthesis via glycine pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from glycine, the pathway betaine biosynthesis via glycine pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei34 – 341S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei43 – 431S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei67 – 671S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei88 – 881S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei132 – 1321S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei134 – 1341SubstratePROSITE-ProRule annotation
Binding sitei167 – 1671SubstratePROSITE-ProRule annotation
Binding sitei206 – 2061SubstratePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • glycine betaine biosynthetic process from glycine Source: UniProtKB

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8542.
BRENDAi2.1.1.156. 2037.
UniPathwayiUPA00530; UER00381.
UPA00530; UER00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine/sarcosine N-methyltransferase (EC:2.1.1.156)
OrganismiHalorhodospira halochloris (Ectothiorhodospira halochloris)
Taxonomic identifieri1052 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeHalorhodospira

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Glycine/sarcosine N-methyltransferasePRO_0000413611Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9KJ22.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni114 – 1152S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18896.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KJ22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTTTEQDFG ADPTKVRDTD HYTEEYVDGF VDKWDDLIDW DSRAKSEGDF
60 70 80 90 100
FIQELKKRGA TRILDAATGT GFHSVRLLEA GFDVVSADGS AEMLAKAFEN
110 120 130 140 150
GRKRGHILRT VQVDWRWLNR DIHGRYDAII CLGNSFTHLF NEKDRRKTLA
160 170 180 190 200
EFYSALNPEG VLILDQRNYD GILDHGYDSS HSYYYCGEGV SVYPEHVDDG
210 220 230 240 250
LARFKYEFND GSTYFLNMFP LRKDYTRRLM HEVGFQKIDT YGDFKATYRD
260
ADPDFFIHVA EKEYREED
Length:268
Mass (Da):31,184
Last modified:October 1, 2000 - v1
Checksum:i6FEF20EB9DA52186
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216281 Genomic DNA. Translation: AAF87202.1.

Genome annotation databases

KEGGiag:AAF87202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216281 Genomic DNA. Translation: AAF87202.1.

3D structure databases

ProteinModelPortaliQ9KJ22.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAF87202.

Phylogenomic databases

KOiK18896.

Enzyme and pathway databases

UniPathwayiUPA00530; UER00381.
UPA00530; UER00382.
BioCyciMetaCyc:MONOMER-8542.
BRENDAi2.1.1.156. 2037.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Extreme halophiles synthesize betaine from glycine by methylation."
    Nyyssola A., Kerovuo J., Kaukinen P., von Weymarn N., Reinikainen T.
    J. Biol. Chem. 275:22196-22201(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase."
    Nyyssola A., Reinikainen T., Leisola M.
    Appl. Environ. Microbiol. 67:2044-2050(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A METHYLTRANSFERASE.FUNCTION IN THE BETAINE BIOSYNTHESIS, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Entry informationi

Entry nameiGSMT_HALHR
AccessioniPrimary (citable) accession number: Q9KJ22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.