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Protein

Glycine/sarcosine/dimethylglycine N-methyltransferase

Gene
N/A
Organism
Actinopolyspora halophila
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylation of glycine, sarcosine and dimethylglycine to sarcosine, dimethylglycine and betaine, respectively, with S-adenosylmethionine (AdoMet) acting as the methyl donor.1 Publication

Catalytic activityi

2 S-adenosyl-L-methionine + glycine = 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine.1 Publication
2 S-adenosyl-L-methionine + sarcosine = 2 S-adenosyl-L-homocysteine + betaine.1 Publication

Pathwayi: betaine biosynthesis via glycine pathway

This protein is involved in step 1, 2 and 3 of the subpathway that synthesizes betaine from glycine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycine/sarcosine/dimethylglycine N-methyltransferase
  2. Glycine/sarcosine/dimethylglycine N-methyltransferase
  3. Glycine/sarcosine/dimethylglycine N-methyltransferase
This subpathway is part of the pathway betaine biosynthesis via glycine pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from glycine, the pathway betaine biosynthesis via glycine pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei53 – 531S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei62 – 621S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei86 – 861S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei107 – 1071S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei152 – 1521S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei154 – 1541SubstratePROSITE-ProRule annotation
Binding sitei187 – 1871SubstratePROSITE-ProRule annotation
Binding sitei226 – 2261SubstratePROSITE-ProRule annotation

GO - Molecular functioni

  • dimethylglycine N-methyltransferase activity Source: UniProtKB
  • glycine N-methyltransferase activity Source: UniProtKB-EC
  • sarcosine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • glycine betaine biosynthetic process from glycine Source: UniProtKB
  • methylation Source: UniProtKB

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8544.
BRENDAi2.1.1.156. 7834.
UniPathwayiUPA00530; UER00381.
UPA00530; UER00382.
UPA00530; UER00383.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine/sarcosine/dimethylglycine N-methyltransferase (EC:2.1.1.156, EC:2.1.1.157)
Alternative name(s):
Dimethylglycine N-methyltransferase
OrganismiActinopolyspora halophila
Taxonomic identifieri1850 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinopolysporalesActinopolysporaceaeActinopolyspora

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Glycine/sarcosine/dimethylglycine N-methyltransferasePRO_0000413613Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9KJ20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 1352S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18896.
K18897.

Family and domain databases

Gene3Di1.10.287.840. 2 hits.
3.40.50.150. 2 hits.
InterProiIPR023143. Cyclopro_synthase-like_dom.
IPR014369. Gly/Sar_N_MeTrfase.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
PfamiPF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KJ20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSVDDLAR GDQAGDEQDP VHREQQTFGD NPLEVRDTDH YMHEYVGGFV
60 70 80 90 100
DKWDDLIDWK KRYESEGSFF IDQLRARGVE TVLDAAAGTG FHSVRLLEEG
110 120 130 140 150
FETVSADGSP QMLAKAFSNG LAYNGHILRV VNADWRWLNR DVHGEYDAII
160 170 180 190 200
CLGNSFTHLF SERDRRKTLA EFYAMLKHDG VLIIDQRNYD SILDTGFSSK
210 220 230 240 250
HTYYYAGEDV SAEPDHIDDG LARFKYTFPD KSEFFLNMYP LRKDYMRRLM
260 270 280 290 300
REVGFQRIDT YGDFQETYGE DEPDFYIHVA EKSYRTEDEF VDMYSNAVHT
310 320 330 340 350
ARDYYNSEDA DNFYYHVWGG NDIHVGLYQT PQEDIATASE RTVQRMAGKV
360 370 380 390 400
DISPETRILD LGAGYGGAAR YLARTYGCHV TCLNLSEVEN QRNREITRAE
410 420 430 440 450
GLEHLIEVTD GSFEDLPYQD NAFDVVWSQD SFLHSGDRSR VMEEVTRVLK
460 470 480 490 500
PKGSVLFTDP MASDSAKKNE LGPILDRLHL DSLGSPGFYR KELTRLGLQN
510 520 530 540 550
IEFEDLSEYL PVHYGRVLEV LESRENELAG FIGEEYRAHM KTGLRNWVQA
560
GNGGSLAWGI IHARA
Length:565
Mass (Da):64,719
Last modified:October 1, 2000 - v1
Checksum:i856F862BC68A149C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216283 Genomic DNA. Translation: AAF87204.1.

Genome annotation databases

KEGGiag:AAF87204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216283 Genomic DNA. Translation: AAF87204.1.

3D structure databases

ProteinModelPortaliQ9KJ20.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAF87204.

Phylogenomic databases

KOiK18896.
K18897.

Enzyme and pathway databases

UniPathwayiUPA00530; UER00381.
UPA00530; UER00382.
UPA00530; UER00383.
BioCyciMetaCyc:MONOMER-8544.
BRENDAi2.1.1.156. 7834.

Family and domain databases

Gene3Di1.10.287.840. 2 hits.
3.40.50.150. 2 hits.
InterProiIPR023143. Cyclopro_synthase-like_dom.
IPR014369. Gly/Sar_N_MeTrfase.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
PfamiPF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSDMT_ACTHA
AccessioniPrimary (citable) accession number: Q9KJ20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.