Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytochrome P450 167A1

Gene

cyp167A1

Organism
Sorangium cellulosum (Polyangium cellulosum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of epothilones, macrolactones which have a narrow anti-fungal spectrum and microtubule-stabilizing activity. Responsible for the epooxidation of epothilones C and D to epothilones A and B, respectively.1 Publication

Catalytic activityi

epothilone C + AH2 + O2 = epothilone A + A + H2O.1 Publication
epothilone D + AH2 + O2 = epothilone B + A + H2O.1 Publication

Cofactori

Pathwayi: epothilone biosynthesis

This protein is involved in the pathway epothilone biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway epothilone biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi365 – 3651Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

  • epothilone biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00774.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 167A1 (EC:1.14.-.-)
Alternative name(s):
Cytochrome P450epoK
Epothilone epoxidase
Gene namesi
Name:cyp167A1
Synonyms:epoF, epoK
OrganismiSorangium cellulosum (Polyangium cellulosum)Imported
Taxonomic identifieri56 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesSorangiineaePolyangiaceaeSorangium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Cytochrome P450 167A1PRO_0000052241Add
BLAST

Structurei

Secondary structure

1
419
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni22 – 265Combined sources
Helixi29 – 3810Combined sources
Beta strandi40 – 445Combined sources
Turni45 – 484Combined sources
Beta strandi49 – 524Combined sources
Helixi55 – 628Combined sources
Helixi71 – 733Combined sources
Helixi77 – 826Combined sources
Helixi85 – 873Combined sources
Helixi88 – 936Combined sources
Turni95 – 973Combined sources
Helixi100 – 11011Combined sources
Helixi111 – 1144Combined sources
Helixi116 – 1194Combined sources
Helixi120 – 1223Combined sources
Helixi123 – 13715Combined sources
Turni145 – 1495Combined sources
Helixi150 – 1523Combined sources
Helixi154 – 1618Combined sources
Helixi166 – 1683Combined sources
Helixi169 – 18214Combined sources
Turni183 – 1875Combined sources
Helixi195 – 21824Combined sources
Helixi225 – 23410Combined sources
Beta strandi235 – 2384Combined sources
Helixi241 – 25515Combined sources
Helixi257 – 27216Combined sources
Helixi274 – 2829Combined sources
Helixi284 – 2863Combined sources
Helixi287 – 29711Combined sources
Beta strandi300 – 31112Combined sources
Beta strandi313 – 3153Combined sources
Beta strandi318 – 3203Combined sources
Beta strandi325 – 3295Combined sources
Helixi330 – 3334Combined sources
Turni337 – 3393Combined sources
Beta strandi340 – 3423Combined sources
Helixi361 – 3633Combined sources
Helixi368 – 38518Combined sources
Beta strandi401 – 4033Combined sources
Beta strandi406 – 4083Combined sources
Beta strandi410 – 4145Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PKFX-ray2.10A1-419[»]
1Q5DX-ray1.93A1-419[»]
1Q5EX-ray2.65A1-419[»]
ProteinModelPortaliQ9KIZ4.
SMRiQ9KIZ4. Positions 13-416.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9KIZ4.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.UniRule annotationCurated

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KIZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQEQANQSE TKPAFDFKPF APGYAEDPFP AIERLREATP IFYWDEGRSW
60 70 80 90 100
VLTRYHDVSA VFRDERFAVS REEWESSAEY SSAIPELSDM KKYGLFGLPP
110 120 130 140 150
EDHARVRKLV NPSFTSRAID LLRAEIQRTV DQLLDARSGQ EEFDVVRDYA
160 170 180 190 200
EGIPMRAISA LLKVPAECDE KFRRFGSATA RALGVGLVPR VDEETKTLVA
210 220 230 240 250
SVTEGLALLH GVLDERRRNP LENDVLTMLL QAEADGSRLS TKELVALVGA
260 270 280 290 300
IIAAGTDTTI YLIAFAVLNL LRSPEALELV KAEPGLMRNA LDEVLRFDNI
310 320 330 340 350
LRIGTVRFAR QDLEYCGASI KKGEMVFLLI PSALRDGTVF SRPDVFDVRR
360 370 380 390 400
DTSASLAYGR GPHVCPGVSL ARLEAEIAVG TIFRRFPEMK LKETPVFGYH
410
PAFRNIESLN VILKPSKAG
Length:419
Mass (Da):46,750
Last modified:October 1, 2000 - v1
Checksum:iFCCE0A193563688F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti190 – 1901R → Q in AAF26924 (PubMed:10662695).Curated
Sequence conflicti211 – 2111G → D in AAF26924 (PubMed:10662695).Curated
Sequence conflicti353 – 3531S → G in AAF26924 (PubMed:10662695).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF210843 Genomic DNA. Translation: AAF26924.1.
AF217189 Genomic DNA. Translation: AAF62886.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF210843 Genomic DNA. Translation: AAF26924.1.
AF217189 Genomic DNA. Translation: AAF62886.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PKFX-ray2.10A1-419[»]
1Q5DX-ray1.93A1-419[»]
1Q5EX-ray2.65A1-419[»]
ProteinModelPortaliQ9KIZ4.
SMRiQ9KIZ4. Positions 13-416.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00774.

Miscellaneous databases

EvolutionaryTraceiQ9KIZ4.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The biosynthetic gene cluster for the microtubule-stabilizing agents epothilones A and B from Sorangium cellulosum So ce90."
    Molnar I., Schupp T., Ono M., Zirkle R.E., Milnamow M., Nowak-Thompson B., Engel N., Toupet C., Stratmann A., Cyr D.D., Gorlach J., Mayo J.M., Hu A., Goff S., Schmid J., Ligon J.M.
    Chem. Biol. 7:97-109(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: So ce90.
  2. "Isolation and characterization of the epothilone biosynthetic gene cluster from Sorangium cellulosum."
    Julien B., Shah S., Ziermann R., Goldman R., Katz L., Khosla C.
    Gene 249:153-160(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: SMP44Imported.
  3. "Cloning and heterologous expression of the epothilone gene cluster."
    Tang L., Shah S., Chung L., Carney J., Katz L., Khosla C., Julien B.
    Science 287:640-642(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SMP44Imported.
  4. "Crystal structures of epothilone D-bound, epothilone B-bound, and substrate-free forms of cytochrome P450epoK."
    Nagano S., Li H., Shimizu H., Nishida C., Ogura H., Ortiz De Montellano P.R., Poulos T.L.
    J. Biol. Chem. 278:44886-44893(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS).

Entry informationi

Entry nameiC167_SORCE
AccessioniPrimary (citable) accession number: Q9KIZ4
Secondary accession number(s): Q9L8C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: October 1, 2000
Last modified: October 14, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.