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Q9KI47

- BGAL_PLASS

UniProt

Q9KI47 - BGAL_PLASS

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Protein

Beta-galactosidase BgaA

Gene

bgaA

Organism
Planococcus sp. (strain 'SOS Orange')
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes o-nitrophenyl-beta-D-galactopyranoside (ONPG), p-nitrophenyl-beta-D-galactopyranoside (PNPG), 5-bromo-4-chloro-3-indoyl-beta-D-galactosde (X-gal), o-nitrophenyl-beta-D-fucopyranoside (ONPF) and p-nitrophenyl-beta-D-fucopyranoside (PNPF) with greatest activity towards ONPG and PNPG and low levels of activity with ONPF and PNPF. Detectable, but very low levels of activity towards p-nitrophenyl-beta-lactose (PNPL), p-nitrophenyl-beta-cellobiose (PNPC), p-nitrophenyl-alpha-galactopyranoside (PNP-alpha-G), and p-nitrophenyl-beta-xylopyranoside (PNPX).1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Enzyme regulationi

No activity is lost during treatment with 20 or 100 mM EDTA in Z buffer for 3 hours at 0 degrees Celsius, nor is activity greatly stimulated by the addition of cations. Inhibited by 1 mM zinc and 1 mM copper, the levels of activity decrease to 10% of the untreated control. Nickel, cobalt and manganese at concentrations of 10 mM decrease enzyme activity to either 40% (for nickel and cobalt) or 60% (for manganese) of the activity in untreated controls. No change in enzyme activity in the presence of calcium and magnesium at concentrations up to 50 mM. EDTA-treated enzyme exhibits a slight increase in relative specific activity when it is assayed in the presence of 50 mM NaCl or 50 mM KCl, it does not exhibit enhanced activity at concentrations greater than 250 mM. Maintains between 20 and 40% of activity in the presence of 4 M NaCl or 4 M KCl, and it is more active in the presence of KCl than in the presence of NaCl. Retains 50% of activity in the presence of 3 M KCl or 2.5 M NaCl.1 Publication

Kineticsi

  1. KM=7.4 mM for ONPG (at 1.9 degrees Celsius and at pH 6.5)1 Publication
  2. KM=4.5 mM for ONPG (at 10 degrees Celsius and at pH 6.5)1 Publication
  3. KM=5.4 mM for ONPG (at 20 degrees Celsius and at pH 6.5)1 Publication
  4. KM=5.0 mM for ONPG (at 30 degrees Celsius and at pH 6.5)1 Publication
  5. KM=4.9 mM for ONPG (at 39 degrees Celsius and at pH 6.5)1 Publication

Vmax=63 µmol/min/mg enzyme with ONPG as substrate (at 1.9 degrees Celsius and pH 6.5)1 Publication

Vmax=80 µmol/min/mg enzyme with ONPG as substrate (at 10 degrees Celsius and pH 6.5)1 Publication

Vmax=223 µmol/min/mg enzyme with ONPG as substrate (at 20 degrees Celsius and pH 6.5)1 Publication

Vmax=392 µmol/min/mg enzyme with ONPG as substrate (at 30 degrees Celsius and pH 6.5)1 Publication

Vmax=467 µmol/min/mg enzyme with ONPG as substrate (at 39 degrees Celsius and pH 6.5)1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Temperature dependencei

Optimum temperature is 42 degrees Celsius. Thermostable at temperatures at or below the optimal temperature for activity, but it is rapidly denatured at temperatures above 42 degrees Celsius. Irreversibly inactivated within 10 minutes at 55 degrees Celsius. Stable during storage at 5 degrees Celsius and loses no activity during storage for 4 months. Retains 10% of activity at 0 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei112 – 1121SubstrateBy similarity
Metal bindingi116 – 1161ZincBy similarity
Binding sitei150 – 1501SubstrateBy similarity
Active sitei151 – 1511Proton donorBy similarity
Metal bindingi156 – 1561ZincBy similarity
Metal bindingi158 – 1581ZincBy similarity
Metal bindingi161 – 1611ZincBy similarity
Active sitei309 – 3091NucleophileBy similarity
Binding sitei317 – 3171SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.2.1.23. 4880.

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase BgaA (EC:3.2.1.23)
Short name:
Beta-galBy similarity
Gene namesi
Name:bgaA1 Publication
OrganismiPlanococcus sp. (strain 'SOS Orange')
Taxonomic identifieri128803 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPlanococcaceaePlanococcus

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Biotechnological usei

Possible reporter enzyme for halotolerant and halophilic organisms. May also be used in the food industry to digest plant polysaccharides in high-salt processes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 677677Beta-galactosidase BgaAPRO_0000407692Add
BLAST

Interactioni

Subunit structurei

Dimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9KI47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni357 – 3604Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KI47-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MINDKLPKIW HGGDYNPEQW DSKEIWDEDV RMFKLAGIDV ATLNVFSWAL
60 70 80 90 100
NQPNEDTYNF DWLDEKINRL YENGIYTCLA TSTAAHPAWM AKKYPDVLRV
110 120 130 140 150
DFYGRKRKFG SRHNSCPNSP TYRKYSERIA ETLAERYKDH PAVLIWHVSN
160 170 180 190 200
EYGGYCYCDN CQDAFRNWLS DKYGTLEKLN KAWNTGFWGH TFYEWDEIVA
210 220 230 240 250
PNMLSEKRED NVSDFQGISL DYRRFQSDRL LDCYKLEYNA IRKHVPTSIP
260 270 280 290 300
ITTNLMGTYP MLDYFKWAKE MDVVSWDNYP SIDTPFSYTA MTHDLMRGLK
310 320 330 340 350
GGKPFMLMEQ TPSQQNWQPY NSLKRPGVMR LWSYQAIGRG ADTILYFQLR
360 370 380 390 400
RSVGACEKYH GAVIEHVGHE HTRVFNEVAQ LGQELNGLSD TLLDARVNAK
410 420 430 440 450
VAIVFDWENR WATELSSGPS VSLDYVNEVH KYYDALYKLN VQVDMIGVEE
460 470 480 490 500
DLSKYDVVIA PVLYMVKEGY AAKVEKFVEN GGTFLTTFFS GIVNETDIVT
510 520 530 540 550
LGGYPGELRK VLGIWAEEID ALHPDETNQI VVKGSRGILS GKYSCNLLFD
560 570 580 590 600
LIHTEGAEAV AEYGSDFYKG MPVLTVNKFG KGKAWYVASS PDAEFLVDFL
610 620 630 640 650
QTVCEEAGVE PLLDVPAGVE TTERVKDGQT YLFVLNHNND EVTIELHGSQ
660 670
YREVLTDEQV SGNLVLKEKG VLILAKV
Length:677
Mass (Da):77,484
Last modified:October 1, 2000 - v1
Checksum:i2A1928DADC945E55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242542 Genomic DNA. Translation: AAF75984.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242542 Genomic DNA. Translation: AAF75984.1 .

3D structure databases

ProteinModelPortali Q9KI47.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 3.2.1.23. 4880.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of a salt-tolerant family 42 beta-galactosidase from a psychrophilic antarctic Planococcus isolate."
    Sheridan P.P., Brenchley J.E.
    Appl. Environ. Microbiol. 66:2438-2444(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, SUBUNIT, BIOTECHNOLOGY.
    Strain: SOS OrangeImported.

Entry informationi

Entry nameiBGAL_PLASS
AccessioniPrimary (citable) accession number: Q9KI47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: October 1, 2000
Last modified: October 1, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3