ID RPOS_COXBU Reviewed; 352 AA. AC Q9KI19; Q7C3E6; DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2000, sequence version 1. DT 27-MAR-2024, entry version 130. DE RecName: Full=RNA polymerase sigma factor RpoS {ECO:0000255|HAMAP-Rule:MF_00959}; DE AltName: Full=Sigma S {ECO:0000255|HAMAP-Rule:MF_00959}; DE AltName: Full=Sigma-38 {ECO:0000255|HAMAP-Rule:MF_00959}; GN Name=rpoS {ECO:0000255|HAMAP-Rule:MF_00959}; GN OrderedLocusNames=CBU_1669; OS Coxiella burnetii (strain RSA 493 / Nine Mile phase I). OC Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales; Coxiellaceae; OC Coxiella. OX NCBI_TaxID=227377; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE. RC STRAIN=RSA 493 / Nine Mile phase I; RX PubMed=11447163; DOI=10.1128/iai.69.8.4874-4883.2001; RA Seshadri R., Samuel J.E.; RT "Characterization of a stress-induced alternate sigma factor, RpoS, of RT Coxiella burnetii and its expression during the development cycle."; RL Infect. Immun. 69:4874-4883(2001). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=RSA 493 / Nine Mile phase I; RX PubMed=12704232; DOI=10.1073/pnas.0931379100; RA Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., RA Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., RA Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., RA Lee K.H., Carty H.A., Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., RA Fraser C.M., Heidelberg J.F.; RT "Complete genome sequence of the Q-fever pathogen, Coxiella burnetii."; RL Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003). CC -!- FUNCTION: Sigma factors are initiation factors that promote the CC attachment of RNA polymerase to specific initiation sites and are then CC released. This sigma factor is the master transcriptional regulator of CC the stationary phase and the general stress response. CC {ECO:0000255|HAMAP-Rule:MF_00959}. CC -!- SUBUNIT: Interacts with the RNA polymerase core enzyme. CC {ECO:0000255|HAMAP-Rule:MF_00959}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00959}. CC -!- DEVELOPMENTAL STAGE: Found in the large cell variant (LCV) stage, very CC little is present in the small cell variant (SCV) stage (at protein CC level). LCVs are more metabolically active than SCVs. CC {ECO:0000269|PubMed:11447163}. CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoS subfamily. CC {ECO:0000255|HAMAP-Rule:MF_00959}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF244357; AAF61172.1; -; Genomic_DNA. DR EMBL; AE016828; AAO91165.1; -; Genomic_DNA. DR RefSeq; NP_820651.1; NC_002971.3. DR RefSeq; WP_010958362.1; NC_002971.4. DR AlphaFoldDB; Q9KI19; -. DR SMR; Q9KI19; -. DR STRING; 227377.CBU_1669; -. DR EnsemblBacteria; AAO91165; AAO91165; CBU_1669. DR GeneID; 1209580; -. DR KEGG; cbu:CBU_1669; -. DR PATRIC; fig|227377.7.peg.1659; -. DR eggNOG; COG0568; Bacteria. DR HOGENOM; CLU_014793_3_5_6; -. DR OrthoDB; 9809557at2; -. DR Proteomes; UP000002671; Chromosome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule. DR GO; GO:0006352; P:DNA-templated transcription initiation; IEA:UniProtKB-UniRule. DR CDD; cd06171; Sigma70_r4; 1. DR Gene3D; 1.10.601.10; RNA Polymerase Primary Sigma Factor; 1. DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2. DR HAMAP; MF_00959; Sigma70_RpoS; 1. DR InterPro; IPR014284; RNA_pol_sigma-70_dom. DR InterPro; IPR000943; RNA_pol_sigma70. DR InterPro; IPR009042; RNA_pol_sigma70_r1_2. DR InterPro; IPR007627; RNA_pol_sigma70_r2. DR InterPro; IPR007624; RNA_pol_sigma70_r3. DR InterPro; IPR007630; RNA_pol_sigma70_r4. DR InterPro; IPR013325; RNA_pol_sigma_r2. DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like. DR InterPro; IPR012761; RNA_pol_sigma_RpoS. DR InterPro; IPR036388; WH-like_DNA-bd_sf. DR NCBIfam; TIGR02394; rpoS_proteo; 1. DR NCBIfam; TIGR02937; sigma70-ECF; 1. DR PANTHER; PTHR30603; RNA POLYMERASE SIGMA FACTOR RPO; 1. DR PANTHER; PTHR30603:SF67; RNA POLYMERASE SIGMA FACTOR RPOS; 1. DR Pfam; PF00140; Sigma70_r1_2; 1. DR Pfam; PF04542; Sigma70_r2; 1. DR Pfam; PF04539; Sigma70_r3; 1. DR Pfam; PF04545; Sigma70_r4; 1. DR PRINTS; PR00046; SIGMA70FCT. DR SUPFAM; SSF88946; Sigma2 domain of RNA polymerase sigma factors; 1. DR SUPFAM; SSF88659; Sigma3 and sigma4 domains of RNA polymerase sigma factors; 2. DR PROSITE; PS00715; SIGMA70_1; 1. PE 1: Evidence at protein level; KW Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription; KW Transcription regulation. FT CHAIN 1..352 FT /note="RNA polymerase sigma factor RpoS" FT /id="PRO_0000318558" FT DNA_BIND 311..330 FT /note="H-T-H motif" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959" FT REGION 1..78 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 79..112 FT /note="Sigma-70 factor domain-1" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959" FT REGION 117..187 FT /note="Sigma-70 factor domain-2" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959" FT REGION 197..272 FT /note="Sigma-70 factor domain-3" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959" FT REGION 285..338 FT /note="Sigma-70 factor domain-4" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00959" FT MOTIF 141..144 FT /note="Interaction with polymerase core subunit RpoC" FT COMPBIAS 1..37 FT /note="Basic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 38..78 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 352 AA; 40471 MW; 35EA31D1C1132251 CRC64; MKTKTTKKTI KKAAKKIKKP SKRKIKKTAK KSRPKKPIKA SDHGLIFAKT KKETTEKEDA ELANAKAKTK KRRETRSSDP TQIYLRELGF QPLLNAKEEL KIARRVHKGD PKARKQMIEA NLRLVVKIAR HYVNRGLPFL DLIEEGNLGL LTAVEKFDPE RGFRFSTYAT WWIRQTIERA IMNQSRTVRL PIHVIKELNV YLRTAKKLTQ EVDHEATPED VAHLIDKPVQ EIRRIMDLAP SATSIDVPIS EDGQKSLVDT LADDNNIDPA RLIQNVDLQD HIERWLAQLD ERHREVVILR FGLHENEKGT LEAVGKAVGL TRERVRQIQI DALQQLRHIL EMEGVTGEEV ED //