Q9KI19 (RPOS_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase sigma factor RpoS

Gene names

Name:	rpoS
Ordered Locus Names:	CBU_1669

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released By similarity.
Developmental stage	Found in the large cell variant (LCV) stage, very little is present in the small cell variant (SCV) stage (at protein level). LCVs are more metabolically active than SCVs. Ref.1
Sequence similarities	Belongs to the sigma-70 factor family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Sigma factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	RNA polymerase sigma factor RpoS		PRO_0000318558
Regions
	DNA binding	311 – 330	20	H-T-H motif By similarity
	Motif	141 – 153	13	Polymerase core binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9KI19 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 35EA31D1C1132251	FASTA	352	40,471
	10 20 30 40 50 60 MKTKTTKKTI KKAAKKIKKP SKRKIKKTAK KSRPKKPIKA SDHGLIFAKT KKETTEKEDA 70 80 90 100 110 120 ELANAKAKTK KRRETRSSDP TQIYLRELGF QPLLNAKEEL KIARRVHKGD PKARKQMIEA 130 140 150 160 170 180 NLRLVVKIAR HYVNRGLPFL DLIEEGNLGL LTAVEKFDPE RGFRFSTYAT WWIRQTIERA 190 200 210 220 230 240 IMNQSRTVRL PIHVIKELNV YLRTAKKLTQ EVDHEATPED VAHLIDKPVQ EIRRIMDLAP 250 260 270 280 290 300 SATSIDVPIS EDGQKSLVDT LADDNNIDPA RLIQNVDLQD HIERWLAQLD ERHREVVILR 310 320 330 340 350 FGLHENEKGT LEAVGKAVGL TRERVRQIQI DALQQLRHIL EMEGVTGEEV ED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a stress-induced alternate sigma factor, RpoS, of Coxiella burnetii and its expression during the development cycle." Seshadri R., Samuel J.E. Infect. Immun. 69:4874-4883(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE. Strain: RSA 493 / Nine Mile phase I.
[2]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF244357 Genomic DNA. Translation: AAF61172.1. AE016828 Genomic DNA. Translation: AAO91165.1.
RefSeq	NP_820651.1. NC_002971.3.
3D structure databases
HSSP	HSSP built from PDB template 1TLH based on UniProtKB P00579.
ProteinModelPortal	Q9KI19.
SMR	Q9KI19. Positions 102-184.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1669.
Proteomic databases
PRIDE	Q9KI19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO91165; AAO91165; CBU_1669.
GeneID	1209580.
KEGG	cbu:CBU_1669.
PATRIC	17932081. VBICoxBur82552_1659.
Phylogenomic databases
eggNOG	COG0568.
HOGENOM	HOG000270273.
KO	K03087.
OMA	HISAQAT.
ProtClustDB	CLSK914949.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1641-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 2 hits.
InterPro	IPR014284. RNA_pol_sigma-70_dom. IPR000943. RNA_pol_sigma70. IPR009042. RNA_pol_sigma70_r1_2. IPR007627. RNA_pol_sigma70_r2. IPR007624. RNA_pol_sigma70_r3. IPR007630. RNA_pol_sigma70_r4. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR012761. RNA_pol_sigma_RpoS_proteobac. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00140. Sigma70_r1_2. 1 hit. PF04542. Sigma70_r2. 1 hit. PF04539. Sigma70_r3. 1 hit. PF04545. Sigma70_r4. 1 hit. [Graphical view]
PRINTS	PR00046. SIGMA70FCT.
SUPFAM	SSF88946. Sigma_r2. 1 hit. SSF88659. Sigma_r3_r4. 2 hits.
TIGRFAMs	TIGR02394. rpoS_proteo. 1 hit. TIGR02937. sigma70-ECF. 1 hit.
PROSITE	PS00715. SIGMA70_1. 1 hit. PS00716. SIGMA70_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOS_COXBU

Accession

Primary (citable) accession number: Q9KI19
Secondary accession number(s): Q7C3E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program