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Q9KI19 (RPOS_COXBU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA polymerase sigma factor RpoS
Alternative name(s):
Sigma S
Sigma-38
Gene names
Name:rpoS
Ordered Locus Names:CBU_1669
OrganismCoxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]
Taxonomic identifier227377 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response By similarity. HAMAP-Rule MF_00959

Subunit structure

Interacts with the RNA polymerase core enzyme By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00959.

Developmental stage

Found in the large cell variant (LCV) stage, very little is present in the small cell variant (SCV) stage (at protein level). LCVs are more metabolically active than SCVs. Ref.1

Sequence similarities

Belongs to the sigma-70 factor family. RpoS subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 352352RNA polymerase sigma factor RpoS HAMAP-Rule MF_00959
PRO_0000318558

Regions

DNA binding311 – 33020H-T-H motif By similarity
Region79 – 11234Sigma-70 factor domain-1 By similarity
Region117 – 18771Sigma-70 factor domain-2 By similarity
Region197 – 27276Sigma-70 factor domain-3 By similarity
Region285 – 33854Sigma-70 factor domain-4 By similarity
Motif141 – 1444Interaction with polymerase core subunit RpoC HAMAP-Rule MF_00959

Sequences

Sequence LengthMass (Da)Tools
Q9KI19 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 35EA31D1C1132251

FASTA35240,471
        10         20         30         40         50         60 
MKTKTTKKTI KKAAKKIKKP SKRKIKKTAK KSRPKKPIKA SDHGLIFAKT KKETTEKEDA 

        70         80         90        100        110        120 
ELANAKAKTK KRRETRSSDP TQIYLRELGF QPLLNAKEEL KIARRVHKGD PKARKQMIEA 

       130        140        150        160        170        180 
NLRLVVKIAR HYVNRGLPFL DLIEEGNLGL LTAVEKFDPE RGFRFSTYAT WWIRQTIERA 

       190        200        210        220        230        240 
IMNQSRTVRL PIHVIKELNV YLRTAKKLTQ EVDHEATPED VAHLIDKPVQ EIRRIMDLAP 

       250        260        270        280        290        300 
SATSIDVPIS EDGQKSLVDT LADDNNIDPA RLIQNVDLQD HIERWLAQLD ERHREVVILR 

       310        320        330        340        350 
FGLHENEKGT LEAVGKAVGL TRERVRQIQI DALQQLRHIL EMEGVTGEEV ED 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a stress-induced alternate sigma factor, RpoS, of Coxiella burnetii and its expression during the development cycle."
Seshadri R., Samuel J.E.
Infect. Immun. 69:4874-4883(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
Strain: RSA 493 / Nine Mile phase I.
[2]"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii."
Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. expand/collapse author list , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F.
Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF244357 Genomic DNA. Translation: AAF61172.1.
AE016828 Genomic DNA. Translation: AAO91165.1.
RefSeqNP_820651.1. NC_002971.3.

3D structure databases

ProteinModelPortalQ9KI19.
SMRQ9KI19. Positions 102-184.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227377.CBU_1669.

Proteomic databases

PRIDEQ9KI19.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO91165; AAO91165; CBU_1669.
GeneID1209580.
KEGGcbu:CBU_1669.
PATRIC17932081. VBICoxBur82552_1659.

Phylogenomic databases

eggNOGCOG0568.
HOGENOMHOG000270273.
KOK03087.
OMANILSPHE.
OrthoDBEOG6XHC70.

Enzyme and pathway databases

BioCycCBUR227377:GJ7S-1641-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 2 hits.
HAMAPMF_00959. Sigma70_RpoS.
InterProIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012761. RNA_pol_sigma_RpoS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSPR00046. SIGMA70FCT.
SUPFAMSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsTIGR02394. rpoS_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEPS00715. SIGMA70_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRPOS_COXBU
AccessionPrimary (citable) accession number: Q9KI19
Secondary accession number(s): Q7C3E6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Coxiella burnetii

Coxiella burnetii (strain RSA 493): entries and gene names