Q9KHV6 (T2A1_RUEGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme AgeI
Short name=R.AgeI
EC=3.1.21.4

Alternative name(s):
Endonuclease AgeI
Type II restriction enzyme AgeI

Gene names

Name:

ageIR

Organism

Ruegeria gelatinovora (Agrobacterium gelatinovorum)

Taxonomic identifier

53501 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Thalassobius

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence ACCGGT and cleaves after A-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Type-2 restriction enzyme AgeI		PRO_0000077278

Sequences

	Sequence		Length	Mass (Da)
	Q9KHV6 [UniParc]. Last modified December 15, 2009. Version 3. Checksum: A02D953D3A30DB32	FASTA	278	30,539
	10 20 30 40 50 60 MRLDLDFGRG LVAHVMLDNV SEEQYQQISD YFVPLVNKPK LKSRDAIGQA FVMATEVCPD 70 80 90 100 110 120 ANPSDLWHHV LYRIYIREKI GTDPSQSWVR TSGEAFEVAL VERYNPVLAR HGIRLTALFK 130 140 150 160 170 180 GQKGLALTRM GVADRVGSRK VDVMIEKQGG GRSPDAEGFG VVGGIHAKVS LAERVSDDIP 190 200 210 220 230 240 ASRIMMGEGL LSVLSTLDVK SFPPPHGDLV NRGELGTPDR PSDKRNYIEG HGDFSACFSY 250 260 270 NLRTSPSNAT TPSGRHIYVS GFSGQDDEFT DYLVAQLA

References

[1]	"Method for cloning and producing AgeI restriction endonuclease in E.coli." Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R. Patent number EP0959131, 26-APR-1999 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM 12617.
[2]	Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO N-TERMINUS.
[3]	Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 245 AND C-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF247972 Genomic DNA. Translation: AAF71526.3.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	42. AgeI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2A1_RUEGE

Accession

Primary (citable) accession number: Q9KHV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	December 15, 2009
Last modified:	July 11, 2012
This is version 30 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program