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Q9KHV6

- T2A1_THAGE

UniProt

Q9KHV6 - T2A1_THAGE

Protein

Type-2 restriction enzyme AgeI

Gene

ageIR

Organism
Thalassobius gelatinovorus (Ruegeria gelatinovora)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 33 (16 Apr 2014)
      Sequence version 3 (15 Dec 2009)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence ACCGGT and cleaves after A-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi42. AgeI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme AgeI (EC:3.1.21.4)
    Short name:
    R.AgeI
    Alternative name(s):
    Endonuclease AgeI
    Type II restriction enzyme AgeI
    Gene namesi
    Name:ageIR
    OrganismiThalassobius gelatinovorus (Ruegeria gelatinovora)
    Taxonomic identifieri53501 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeThalassobius

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 278278Type-2 restriction enzyme AgeIPRO_0000077278Add
    BLAST

    Sequencei

    Sequence statusi: Complete.

    Q9KHV6-1 [UniParc]FASTAAdd to Basket

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    MRLDLDFGRG LVAHVMLDNV SEEQYQQISD YFVPLVNKPK LKSRDAIGQA    50
    FVMATEVCPD ANPSDLWHHV LYRIYIREKI GTDPSQSWVR TSGEAFEVAL 100
    VERYNPVLAR HGIRLTALFK GQKGLALTRM GVADRVGSRK VDVMIEKQGG 150
    GRSPDAEGFG VVGGIHAKVS LAERVSDDIP ASRIMMGEGL LSVLSTLDVK 200
    SFPPPHGDLV NRGELGTPDR PSDKRNYIEG HGDFSACFSY NLRTSPSNAT 250
    TPSGRHIYVS GFSGQDDEFT DYLVAQLA 278
    Length:278
    Mass (Da):30,539
    Last modified:December 15, 2009 - v3
    Checksum:iA02D953D3A30DB32
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF247972 Genomic DNA. Translation: AAF71526.3.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF247972 Genomic DNA. Translation: AAF71526.3 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 42. AgeI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Method for cloning and producing AgeI restriction endonuclease in E.coli."
      Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
      Patent number EP0959131, 26-APR-1999
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.
    2. Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO N-TERMINUS.
    3. Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
      Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 245 AND C-TERMINUS.

    Entry informationi

    Entry nameiT2A1_THAGE
    AccessioniPrimary (citable) accession number: Q9KHV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 8, 2000
    Last sequence update: December 15, 2009
    Last modified: April 16, 2014
    This is version 33 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3