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Q9KHV6

- T2A1_THAGE

UniProt

Q9KHV6 - T2A1_THAGE

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Protein
Type-2 restriction enzyme AgeI
Gene
ageIR
Organism
Thalassobius gelatinovorus (Ruegeria gelatinovora)
Status
Reviewed - Annotation score: 2 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequence ACCGGT and cleaves after A-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi42. AgeI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme AgeI (EC:3.1.21.4)
Short name:
R.AgeI
Alternative name(s):
Endonuclease AgeI
Type II restriction enzyme AgeI
Gene namesi
Name:ageIR
OrganismiThalassobius gelatinovorus (Ruegeria gelatinovora)
Taxonomic identifieri53501 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeThalassobius

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278Type-2 restriction enzyme AgeI
PRO_0000077278Add
BLAST

Sequencei

Sequence statusi: Complete.

Q9KHV6-1 [UniParc]FASTAAdd to Basket

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MRLDLDFGRG LVAHVMLDNV SEEQYQQISD YFVPLVNKPK LKSRDAIGQA    50
FVMATEVCPD ANPSDLWHHV LYRIYIREKI GTDPSQSWVR TSGEAFEVAL 100
VERYNPVLAR HGIRLTALFK GQKGLALTRM GVADRVGSRK VDVMIEKQGG 150
GRSPDAEGFG VVGGIHAKVS LAERVSDDIP ASRIMMGEGL LSVLSTLDVK 200
SFPPPHGDLV NRGELGTPDR PSDKRNYIEG HGDFSACFSY NLRTSPSNAT 250
TPSGRHIYVS GFSGQDDEFT DYLVAQLA 278
Length:278
Mass (Da):30,539
Last modified:December 15, 2009 - v3
Checksum:iA02D953D3A30DB32
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF247972 Genomic DNA. Translation: AAF71526.3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF247972 Genomic DNA. Translation: AAF71526.3 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 42. AgeI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Method for cloning and producing AgeI restriction endonuclease in E.coli."
    Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
    Patent number EP0959131, 26-APR-1999
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.
  2. Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  3. Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 245 AND C-TERMINUS.

Entry informationi

Entry nameiT2A1_THAGE
AccessioniPrimary (citable) accession number: Q9KHV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: December 15, 2009
Last modified: April 16, 2014
This is version 33 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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