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Protein

Type-2 restriction enzyme AgeI

Gene

ageIR

Organism
Thalassobius gelatinovorus (Ruegeria gelatinovora)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence ACCGGT and cleaves after A-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Enzyme and pathway databases

BRENDAi3.1.21.4. 201.

Protein family/group databases

REBASEi42. AgeI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme AgeI (EC:3.1.21.4)
Short name:
R.AgeI
Alternative name(s):
Endonuclease AgeI
Type II restriction enzyme AgeI
Gene namesi
Name:ageIR
OrganismiThalassobius gelatinovorus (Ruegeria gelatinovora)
Taxonomic identifieri53501 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeThalassobius

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278Type-2 restriction enzyme AgeIPRO_0000077278Add
BLAST

Sequencei

Sequence statusi: Complete.

Q9KHV6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLDLDFGRG LVAHVMLDNV SEEQYQQISD YFVPLVNKPK LKSRDAIGQA
60 70 80 90 100
FVMATEVCPD ANPSDLWHHV LYRIYIREKI GTDPSQSWVR TSGEAFEVAL
110 120 130 140 150
VERYNPVLAR HGIRLTALFK GQKGLALTRM GVADRVGSRK VDVMIEKQGG
160 170 180 190 200
GRSPDAEGFG VVGGIHAKVS LAERVSDDIP ASRIMMGEGL LSVLSTLDVK
210 220 230 240 250
SFPPPHGDLV NRGELGTPDR PSDKRNYIEG HGDFSACFSY NLRTSPSNAT
260 270
TPSGRHIYVS GFSGQDDEFT DYLVAQLA
Length:278
Mass (Da):30,539
Last modified:December 15, 2009 - v3
Checksum:iA02D953D3A30DB32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247972 Genomic DNA. Translation: AAF71526.3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247972 Genomic DNA. Translation: AAF71526.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi42. AgeI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.21.4. 201.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Method for cloning and producing AgeI restriction endonuclease in E.coli."
    Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
    Patent number EP0959131, 26-APR-1999
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.
  2. Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  3. Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 245 AND C-TERMINUS.

Entry informationi

Entry nameiT2A1_THAGE
AccessioniPrimary (citable) accession number: Q9KHV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: December 15, 2009
Last modified: April 1, 2015
This is version 34 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.