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Protein

Type-2 restriction enzyme AgeI

Gene

ageIR

Organism
Thalassobius gelatinovorus (Ruegeria gelatinovora)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence ACCGGT and cleaves after A-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system

Enzyme and pathway databases

BRENDAi3.1.21.4. 201.

Protein family/group databases

REBASEi42. AgeI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme AgeI (EC:3.1.21.4)
Short name:
R.AgeI
Alternative name(s):
Endonuclease AgeI
Type II restriction enzyme AgeI
Gene namesi
Name:ageIR
OrganismiThalassobius gelatinovorus (Ruegeria gelatinovora)
Taxonomic identifieri53501 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeThalassobius

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000772781 – 278Type-2 restriction enzyme AgeIAdd BLAST278

Structurei

Secondary structure

1278
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Beta strandi11 – 16Combined sources6
Helixi22 – 36Combined sources15
Helixi43 – 57Combined sources15
Helixi63 – 69Combined sources7
Helixi71 – 78Combined sources8
Helixi84 – 109Combined sources26
Turni110 – 112Combined sources3
Beta strandi113 – 117Combined sources5
Turni120 – 122Combined sources3
Helixi123 – 130Combined sources8
Turni133 – 135Combined sources3
Turni136 – 139Combined sources4
Beta strandi143 – 148Combined sources6
Beta strandi151 – 153Combined sources3
Beta strandi158 – 169Combined sources12
Helixi174 – 177Combined sources4
Helixi178 – 187Combined sources10
Beta strandi191 – 196Combined sources6
Turni204 – 206Combined sources3
Beta strandi218 – 221Combined sources4
Helixi223 – 229Combined sources7
Beta strandi234 – 239Combined sources6
Beta strandi252 – 254Combined sources3
Beta strandi257 – 259Combined sources3
Beta strandi262 – 266Combined sources5
Helixi268 – 277Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DWAX-ray1.50A/B1-278[»]
5DWBX-ray2.40A/B1-278[»]
5DWCX-ray2.50A1-278[»]
SMRiQ9KHV6.
ModBaseiSearch...
MobiDBiSearch...

Sequencei

Sequence statusi: Complete.

Q9KHV6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLDLDFGRG LVAHVMLDNV SEEQYQQISD YFVPLVNKPK LKSRDAIGQA
60 70 80 90 100
FVMATEVCPD ANPSDLWHHV LYRIYIREKI GTDPSQSWVR TSGEAFEVAL
110 120 130 140 150
VERYNPVLAR HGIRLTALFK GQKGLALTRM GVADRVGSRK VDVMIEKQGG
160 170 180 190 200
GRSPDAEGFG VVGGIHAKVS LAERVSDDIP ASRIMMGEGL LSVLSTLDVK
210 220 230 240 250
SFPPPHGDLV NRGELGTPDR PSDKRNYIEG HGDFSACFSY NLRTSPSNAT
260 270
TPSGRHIYVS GFSGQDDEFT DYLVAQLA
Length:278
Mass (Da):30,539
Last modified:December 15, 2009 - v3
Checksum:iA02D953D3A30DB32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247972 Genomic DNA. Translation: AAF71526.3.
RefSeqiWP_058263487.1. NZ_CYSA01000025.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247972 Genomic DNA. Translation: AAF71526.3.
RefSeqiWP_058263487.1. NZ_CYSA01000025.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DWAX-ray1.50A/B1-278[»]
5DWBX-ray2.40A/B1-278[»]
5DWCX-ray2.50A1-278[»]
SMRiQ9KHV6.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi42. AgeI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.21.4. 201.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiT2A1_THAGE
AccessioniPrimary (citable) accession number: Q9KHV6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: December 15, 2009
Last modified: January 18, 2017
This is version 37 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.