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Q9KHV6 (T2A1_THAGE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme AgeI

Short name=R.AgeI
EC=3.1.21.4
Alternative name(s):
Endonuclease AgeI
Type II restriction enzyme AgeI
Gene names
Name:ageIR
OrganismThalassobius gelatinovorus (Ruegeria gelatinovora)
Taxonomic identifier53501 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeThalassobius

Protein attributes

Sequence length278 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence ACCGGT and cleaves after A-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 278278Type-2 restriction enzyme AgeI
PRO_0000077278

Sequences

Sequence LengthMass (Da)Tools
Q9KHV6 [UniParc].

Last modified December 15, 2009. Version 3.
Checksum: A02D953D3A30DB32

FASTA27830,539
        10         20         30         40         50         60 
MRLDLDFGRG LVAHVMLDNV SEEQYQQISD YFVPLVNKPK LKSRDAIGQA FVMATEVCPD 

        70         80         90        100        110        120 
ANPSDLWHHV LYRIYIREKI GTDPSQSWVR TSGEAFEVAL VERYNPVLAR HGIRLTALFK 

       130        140        150        160        170        180 
GQKGLALTRM GVADRVGSRK VDVMIEKQGG GRSPDAEGFG VVGGIHAKVS LAERVSDDIP 

       190        200        210        220        230        240 
ASRIMMGEGL LSVLSTLDVK SFPPPHGDLV NRGELGTPDR PSDKRNYIEG HGDFSACFSY 

       250        260        270 
NLRTSPSNAT TPSGRHIYVS GFSGQDDEFT DYLVAQLA 

« Hide

References

[1]"Method for cloning and producing AgeI restriction endonuclease in E.coli."
Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
Patent number EP0959131, 26-APR-1999
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 25655 / DSM 5887 / IAM 12617 / JCM 20688 / NBRC 15761 / NCIMB / B6.
[2]Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO N-TERMINUS.
[3]Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 245 AND C-TERMINUS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF247972 Genomic DNA. Translation: AAF71526.3.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE42. AgeI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2A1_THAGE
AccessionPrimary (citable) accession number: Q9KHV6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: December 15, 2009
Last modified: April 16, 2014
This is version 33 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries