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Protein

Carnitine transport ATP-binding protein OpuCA

Gene

opuCA

Organism
Listeria monocytogenes
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex OpuCABCD involved in carnitine uptake. Probably responsible for energy coupling to the transport system. Involved, with BetL and GbuABC, in osmoprotection and cryoprotection of Listeria.1 Publication

Catalytic activityi

ATP + H2O + quaternary amine(Out) = ADP + phosphate + quaternary amine(In).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi35 – 42ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Stress response, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Carnitine transport ATP-binding protein OpuCA (EC:3.6.3.32)
Gene namesi
Name:opuCA
OrganismiListeria monocytogenes
Taxonomic identifieri1639 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mutants are impaired for growth at high osmolarity in brain heart infusion broth, fail to grow in a defined medium and show no detectable carnitine uptake.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004181321 – 397Carnitine transport ATP-binding protein OpuCAAdd BLAST397

Proteomic databases

PaxDbiQ9KHT9.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (OpuCA), two transmembrane proteins (OpuCB and OpuCD) and a solute-binding protein (OpuCC).1 Publication

Protein-protein interaction databases

STRINGi169963.lmo1428.

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi251 – 254Combined sources4
Beta strandi255 – 257Combined sources3
Helixi268 – 278Combined sources11
Beta strandi281 – 286Combined sources6
Beta strandi291 – 297Combined sources7
Helixi298 – 304Combined sources7
Helixi305 – 307Combined sources3
Helixi311 – 314Combined sources4
Helixi328 – 338Combined sources11
Beta strandi343 – 346Combined sources4
Beta strandi351 – 356Combined sources6
Helixi359 – 367Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5KS7X-ray2.90A/B247-372[»]
ProteinModelPortaliQ9KHT9.
SMRiQ9KHT9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 236ABC transporterPROSITE-ProRule annotationAdd BLAST235
Domaini255 – 311CBS 1PROSITE-ProRule annotationAdd BLAST57
Domaini315 – 373CBS 2PROSITE-ProRule annotationAdd BLAST59

Sequence similaritiesi

Belongs to the ABC transporter superfamily.Curated
Contains 1 ABC transporter domain.PROSITE-ProRule annotation
Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiENOG4108IJ8. Bacteria.
COG1125. LUCA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005892. Gly-betaine_transp_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF00571. CBS. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01186. proV. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KHT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFEHVTKT YKGGKKAVND LTLNIDKGEF VCFIGPSGCG KTTTMKMINR
60 70 80 90 100
LIEPTEGKIF INDKDIMAED PVKLRRSIGY VIQQIGLMPH MTIRENIVLV
110 120 130 140 150
PKLLKWSEEK KQERAKELIK LVDLPEEFLD RYPYELSGGQ QQRIGVLRAL
160 170 180 190 200
AAEQNLILMD EPFGALDPIT RDSLQEEFKN LQKELGKTII FVTHDMDEAI
210 220 230 240 250
KLADRIVIMK DGEIVQFDTP DEILRNPANS FVEDFIGKDR LIEAKPDVTQ
260 270 280 290 300
VAQIMNTNPV SITADKSLQA AITVMKEKRV DTLLVVDEGN VLKGFIDVEQ
310 320 330 340 350
IDLNRRTATS VMDIIEKNVF YVYEDTLLRD TVQRILKRGY KYIPVVDKDK
360 370 380 390
RLVGIVTRAS LVDIVYDSIW GTLEDATENQ EEQADSKTTE PEMKQEG
Length:397
Mass (Da):45,209
Last modified:October 1, 2000 - v1
Checksum:i134CF427650AC83B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF249729 Genomic DNA. Translation: AAF91339.1.
PIRiAD1253.
RefSeqiWP_003721933.1. NZ_MDRT01000005.1.

Genome annotation databases

PATRICi20311981. VBILisMon69206_1467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF249729 Genomic DNA. Translation: AAF91339.1.
PIRiAD1253.
RefSeqiWP_003721933.1. NZ_MDRT01000005.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5KS7X-ray2.90A/B247-372[»]
ProteinModelPortaliQ9KHT9.
SMRiQ9KHT9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo1428.

Proteomic databases

PaxDbiQ9KHT9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

PATRICi20311981. VBILisMon69206_1467.

Phylogenomic databases

eggNOGiENOG4108IJ8. Bacteria.
COG1125. LUCA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005892. Gly-betaine_transp_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF00571. CBS. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01186. proV. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOPUCA_LISMN
AccessioniPrimary (citable) accession number: Q9KHT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2012
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.