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Q9KH33

- IMDH_RHITR

UniProt

Q9KH33 - IMDH_RHITR

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Protein
Inosine-5'-monophosphate dehydrogenase
Gene
guaB
Organism
Rhizobium tropici
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth By similarity.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

Potassium By similarity.UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei253 – 2531NAD By similarity
Metal bindingi305 – 3051Potassium; via carbonyl oxygen By similarity
Metal bindingi307 – 3071Potassium; via carbonyl oxygen By similarity
Binding sitei308 – 3081IMP By similarity
Active sitei310 – 3101Thioimidate intermediate By similarity
Metal bindingi310 – 3101Potassium; via carbonyl oxygen By similarity
Binding sitei421 – 4211IMP By similarity
Metal bindingi475 – 4751Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal bindingi476 – 4761Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal bindingi477 – 4771Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi303 – 3053NAD By similarity

GO - Molecular functioni

  1. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  2. adenyl nucleotide binding Source: InterPro
  3. metal ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenase (EC:1.1.1.205)
Short name:
IMP dehydrogenase
Short name:
IMPD
Short name:
IMPDH
Gene namesi
Name:guaB
OrganismiRhizobium tropici
Taxonomic identifieri398 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Inosine-5'-monophosphate dehydrogenaseUniRule annotation
PRO_0000093704Add
BLAST

Proteomic databases

PRIDEiQ9KH33.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ9KH33.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini98 – 15558CBS 1
Add
BLAST
Domaini159 – 21658CBS 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni343 – 3453IMP binding By similarity
Regioni366 – 3672IMP binding By similarity
Regioni390 – 3945IMP binding By similarity

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains.

Keywords - Domaini

CBS domain, Repeat

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KH33-1 [UniParc]FASTAAdd to Basket

« Hide

MARIIETSTG LDALTFDDVL LQPGHSEVMP GQTNIATRIA QDIELNVPIL    50
SAAMDTVTES RLAIAMAQAG GMGVIHRNLT PVQQAEEVRQ VKKFESGMVV 100
NPVTIGPDAT LAEALSLDEG PRHFRASPVV EKSHRLVGIL TNRDVRFASD 150
PEQKIYELMT RENLVTVKDG VQQHEAKRLL HTHRIEKXLV VDADSRFVGL 200
ITVKDIEKSQ LNPHASKDAQ GRLRAAAAIS VGDDGYERAE RLIDAGVDLL 250
VVDTAHGHSQ RVLDAVTRVK KLSNSVRIMA GNVATYDGTR ALIDAGADAV 300
KVGIGPGSIC TTRIVAGVGV PQLAAIMSAV QAAQDQNIPI IADGGIKFSG 350
DLAKAIAAGA SAAMIGSLLA GTDESPGEVY LYQGRSFKAY RGMGSVGAMA 400
RGSADRYFQA EVRDTLKLVP EGIEGXVPYK GPVSGVLHQL AGGLKAAMGY 450
VGGADLKDFQ ERATFVRISG AGLRESHAHD VTITPRKARI IPAQAADR 498
Length:498
Mass (Da):52,838
Last modified:October 1, 2000 - v1
Checksum:i0A99E38B1078ED73
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF272827 Genomic DNA. Translation: AAF85967.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF272827 Genomic DNA. Translation: AAF85967.1 .

3D structure databases

ProteinModelPortali Q9KH33.
ModBasei Search...

Proteomic databases

PRIDEi Q9KH33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00601 ; UER00295 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01964. IMPDH.
InterProi IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view ]
PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
Pfami PF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000130. IMPDH. 1 hit.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
PROSITEi PS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A guaB mutant strain of Rhizobium tropici CIAT899 pleiotropically defective in thermal tolerance and symbiosis."
    Riccillo P.M., Collavino M.M., Grasso D.H., England R., de Bruijn F.J., Aguilar O.M.
    Mol. Plant Microbe Interact. 13:1228-1236(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 899.

Entry informationi

Entry nameiIMDH_RHITR
AccessioniPrimary (citable) accession number: Q9KH33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 1, 2000
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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