Reviewed, UniProtKB/Swiss-Prot Q9KH19 (MHPB_RHOSO)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
    EC=1.13.11.16

Gene names

Name:	mhpB
Synonyms:	ohpD

Organism

Rhodococcus sp.

Taxonomic identifier

1831 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively. Also catalyzes the cleavage of catechol. HAMAP MF_01653
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. Ref.1 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. Ref.1
Cofactor	Fe2+ ion By similarity. HAMAP MF_01653
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity. HAMAP MF_01653
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.
Biophysicochemical properties	Kinetic parameters: KM=30 µM for 3-(2,3-dihydroxyphenyl) propionic acid (at pH 8.1 and 8 degrees Celsius) HAMAP MF_01653 KM=47 µM for catechol (at pH 8.1 and 8 degrees Celsius) KM=62 µM for 3-methylcatechol (at pH 8.1 and 8 degrees Celsius)

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653		PRO_0000337667
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KH19-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C919C10770527E64	FASTA	314	33,916
	10 20 30 40 50 60 MPVALCAMSH SPLMGRNDPE QEVIDAVDAA FDHARRFVAD FAPDLIVIFA PDHYNGVFYD 70 80 90 100 110 120 LLPPFCIGAA AQSVGDYGTE AGPLDVDRDA AYAVARDVLD SGIDVAFSER MHVDHGFAQA 130 140 150 160 170 180 LQLLVGSITA VPTVPIFINS VAEPLGPVSR VRLLGEAVGR AAAKLDKRVL FVGSGGLSHD 190 200 210 220 230 240 PPVPQFATAP EEVRERLIDG RNPSAAERDA REQRVITAGR DFAAGTAAIQ PLNPEWDRHL 250 260 270 280 290 300 LDVLASGDLE QIDAWTNDWF VEQAGHSSHE VRTWIAAYAA MSAAGKYRVT STFYREIHEW 310 IAGFGITTAV AVDE

References

[1]	"Molecular characterisation of a Rhodococcus ohp operon." Powell J.A., Archer J.A. Antonie Van Leeuwenhoek 74:175-188(1998) [PubMed: 10068799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. Strain: ATCC 19070 / V49.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF274045 Genomic DNA. Translation: AAF81826.1.
3D structure databases
SMR	Q9KH19. Positions 4-307.
ModBase	Search...
Family and domain databases
HAMAP	MF_01653. MhpB. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB_RHOSO

Accession

Primary (citable) accession number: Q9KH19

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)