SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9KGN8

- IMDH_BACHD

UniProt

Q9KGN8 - IMDH_BACHD

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Inosine-5'-monophosphate dehydrogenase
Gene
guaB, BH0020
Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth By similarity.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

Potassium By similarity.UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei249 – 2491NAD By similarity
Metal bindingi301 – 3011Potassium; via carbonyl oxygen By similarity
Metal bindingi303 – 3031Potassium; via carbonyl oxygen By similarity
Binding sitei304 – 3041IMP By similarity
Active sitei306 – 3061Thioimidate intermediate By similarity
Metal bindingi306 – 3061Potassium; via carbonyl oxygen By similarity
Binding sitei414 – 4141IMP By similarity
Metal bindingi468 – 4681Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal bindingi469 – 4691Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity
Metal bindingi470 – 4701Potassium; via carbonyl oxygen; shared with tetrameric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi299 – 3013NAD By similarity

GO - Molecular functioni

  1. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  2. adenyl nucleotide binding Source: InterPro
  3. metal ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-23-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenase (EC:1.1.1.205)
Short name:
IMP dehydrogenase
Short name:
IMPD
Short name:
IMPDH
Gene namesi
Name:guaB
Ordered Locus Names:BH0020
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001258: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Inosine-5'-monophosphate dehydrogenaseUniRule annotation
PRO_0000093690Add
BLAST

Proteomic databases

PRIDEiQ9KGN8.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272558.BH0020.

Structurei

3D structure databases

ProteinModelPortaliQ9KGN8.
SMRiQ9KGN8. Positions 2-484.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini95 – 15460CBS 1
Add
BLAST
Domaini155 – 21561CBS 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni339 – 3413IMP binding By similarity
Regioni362 – 3632IMP binding By similarity
Regioni386 – 3905IMP binding By similarity

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains.

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiCOG0517.
KOiK00088.
OMAiHGHSKNI.
OrthoDBiEOG6GTZPV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KGN8-1 [UniParc]FASTAAdd to Basket

« Hide

MWENKFQKEG LTFDDVLLVP AKSEVLPRDV SVKTKLTETL QLNIPIISAG    50
MDTVTEAKMA IAIAREGGLG IIHKNMSVEE QAEQVDRVKR SESGVITNPF 100
FLTPDRQVFD AEHLMGKYRI SGVPIVDEDQ KLVGILTNRD LRFIEDYSTL 150
IDDVMTKENL VTAPVGTTLK EAEEILQKHK IEKLPLVDES GTLKGLITIK 200
DIEKVIEFPN SAKDSQGRLI VGAAVGVSAD TDVRVAALVE AGVDVIVIDT 250
AHGHSKGVLE KVKAIREQYP DLTIIAGNVA TAEATRDLIE AGANVVKVGI 300
GPGSICTTRI VAGIGVPQIT AVYDCANEAR KHGVPIIADG GIKYSGDIVK 350
ALAAGGHAVM LGSLLAGVSE SPGEREIFQG RQFKVYRGMG SLGAMEKGSK 400
DRYFQENNQK LVPEGIEGRI PYKGPLHDTI HQLVGGIRAG MGYCGTKTID 450
ELRENTQFIR ITGAGLRESH PHDVQITKEA PNYTL 485
Length:485
Mass (Da):52,429
Last modified:October 1, 2000 - v1
Checksum:iD0B64489E5CF8B60
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000004 Genomic DNA. Translation: BAB03739.1.
PIRiD83652.
RefSeqiNP_240886.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB03739; BAB03739; BAB03739.
GeneIDi890466.
KEGGibha:BH0020.
PATRICi18936854. VBIBacHal18977_0021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000004 Genomic DNA. Translation: BAB03739.1 .
PIRi D83652.
RefSeqi NP_240886.1. NC_002570.2.

3D structure databases

ProteinModelPortali Q9KGN8.
SMRi Q9KGN8. Positions 2-484.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272558.BH0020.

Proteomic databases

PRIDEi Q9KGN8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB03739 ; BAB03739 ; BAB03739 .
GeneIDi 890466.
KEGGi bha:BH0020.
PATRICi 18936854. VBIBacHal18977_0021.

Phylogenomic databases

eggNOGi COG0517.
KOi K00088.
OMAi HGHSKNI.
OrthoDBi EOG6GTZPV.

Enzyme and pathway databases

UniPathwayi UPA00601 ; UER00295 .
BioCyci BHAL272558:GJC5-23-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01964. IMPDH.
InterProi IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view ]
Pfami PF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000130. IMPDH. 1 hit.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
PROSITEi PS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiIMDH_BACHD
AccessioniPrimary (citable) accession number: Q9KGN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi