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Q9KGN8

- IMDH_BACHD

UniProt

Q9KGN8 - IMDH_BACHD

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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K(+)UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei249 – 2491NADUniRule annotation
Metal bindingi301 – 3011Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi303 – 3031Potassium; via carbonyl oxygenUniRule annotation
Binding sitei304 – 3041IMPUniRule annotation
Active sitei306 – 3061Thioimidate intermediateUniRule annotation
Metal bindingi306 – 3061Potassium; via carbonyl oxygenUniRule annotation
Binding sitei414 – 4141IMPUniRule annotation
Metal bindingi468 – 4681Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi469 – 4691Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi470 – 4701Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi299 – 3013NADUniRule annotation

GO - Molecular functioni

  1. adenyl nucleotide binding Source: InterPro
  2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-23-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotation
Ordered Locus Names:BH0020
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001258: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Inosine-5'-monophosphate dehydrogenasePRO_0000093690Add
BLAST

Proteomic databases

PRIDEiQ9KGN8.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi272558.BH0020.

Structurei

3D structure databases

ProteinModelPortaliQ9KGN8.
SMRiQ9KGN8. Positions 2-484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini95 – 15460CBS 1UniRule annotationAdd
BLAST
Domaini155 – 21561CBS 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni339 – 3413IMP bindingUniRule annotation
Regioni362 – 3632IMP bindingUniRule annotation
Regioni386 – 3905IMP bindingUniRule annotation

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation
Contains 2 CBS domains.UniRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiCOG0517.
KOiK00088.
OMAiHGHSKNI.
OrthoDBiEOG6GTZPV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9KGN8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWENKFQKEG LTFDDVLLVP AKSEVLPRDV SVKTKLTETL QLNIPIISAG
60 70 80 90 100
MDTVTEAKMA IAIAREGGLG IIHKNMSVEE QAEQVDRVKR SESGVITNPF
110 120 130 140 150
FLTPDRQVFD AEHLMGKYRI SGVPIVDEDQ KLVGILTNRD LRFIEDYSTL
160 170 180 190 200
IDDVMTKENL VTAPVGTTLK EAEEILQKHK IEKLPLVDES GTLKGLITIK
210 220 230 240 250
DIEKVIEFPN SAKDSQGRLI VGAAVGVSAD TDVRVAALVE AGVDVIVIDT
260 270 280 290 300
AHGHSKGVLE KVKAIREQYP DLTIIAGNVA TAEATRDLIE AGANVVKVGI
310 320 330 340 350
GPGSICTTRI VAGIGVPQIT AVYDCANEAR KHGVPIIADG GIKYSGDIVK
360 370 380 390 400
ALAAGGHAVM LGSLLAGVSE SPGEREIFQG RQFKVYRGMG SLGAMEKGSK
410 420 430 440 450
DRYFQENNQK LVPEGIEGRI PYKGPLHDTI HQLVGGIRAG MGYCGTKTID
460 470 480
ELRENTQFIR ITGAGLRESH PHDVQITKEA PNYTL
Length:485
Mass (Da):52,429
Last modified:October 1, 2000 - v1
Checksum:iD0B64489E5CF8B60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB03739.1.
PIRiD83652.
RefSeqiNP_240886.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB03739; BAB03739; BAB03739.
GeneIDi890466.
KEGGibha:BH0020.
PATRICi18936854. VBIBacHal18977_0021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB03739.1 .
PIRi D83652.
RefSeqi NP_240886.1. NC_002570.2.

3D structure databases

ProteinModelPortali Q9KGN8.
SMRi Q9KGN8. Positions 2-484.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272558.BH0020.

Proteomic databases

PRIDEi Q9KGN8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB03739 ; BAB03739 ; BAB03739 .
GeneIDi 890466.
KEGGi bha:BH0020.
PATRICi 18936854. VBIBacHal18977_0021.

Phylogenomic databases

eggNOGi COG0517.
KOi K00088.
OMAi HGHSKNI.
OrthoDBi EOG6GTZPV.

Enzyme and pathway databases

UniPathwayi UPA00601 ; UER00295 .
BioCyci BHAL272558:GJC5-23-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01964. IMPDH.
InterProi IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view ]
Pfami PF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000130. IMPDH. 1 hit.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
PROSITEi PS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiIMDH_BACHD
AccessioniPrimary (citable) accession number: Q9KGN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3