Q9KGK8 (SYM_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Synonyms:	metS
Ordered Locus Names:	BH0053

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_01228
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228
Subunit structure	Homodimer By similarity. HAMAP MF_01228
Subcellular location	Cytoplasm HAMAP MF_01228.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Methionine--tRNA ligase HAMAP MF_01228		PRO_0000139209
Regions
	Domain	560 – 660	101	tRNA-binding
	Motif	15 – 25	11	"HIGH" region HAMAP MF_01228
	Motif	311 – 315	5	"KMSKS" region HAMAP MF_01228
Sites
	Binding site	314	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KGK8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: EC3408645728A536	FASTA	660	75,192
	10 20 30 40 50 60 MSKEQKTFYL TTPIYYPSDK LHIGHAYTTV AGDAMARYKR LRGYNVMYLT GTDEHGQKIE 70 80 90 100 110 120 QKAEAKGLTP KQFVDEIVSG IQDLWKKLDI SYDDFIRTTE ERHIKVVQQI FEQLLDQGDI 130 140 150 160 170 180 YLGDYEGWYS IPDETFYTET QLVDKEYDAD GNLIGGKSPD SGHPVEKVRE QSYFFRMSKY 190 200 210 220 230 240 ADRLLQFYEE NPQFIQPESR KNEMINNFIK PGLEDLAVSR TSFTWGVPVP RDPKHVVYVW 250 260 270 280 290 300 IDALSNYITA LGYGSDQQEK YENYWPADVH LVGKEIVRFH TIYWPIMLMA LDLPLPKKVF 310 320 330 340 350 360 GHGWLLMKDG KMSKSKGNVV DPVPLIDRYG LDALRYYLLR EVPFGSDGVF TPEAFVERVN 370 380 390 400 410 420 YDLANDLGNL LNRTVAMIEK YFDGKIPSYV KDGTPFDADL VELVYSTVKK VEDAMEEMEF 430 440 450 460 470 480 SIALTAIGQL ISRTNKYIDE TQPWVLAKDE SKREQLGAAM YHLAESIRYV SVLLQPFMTK 490 500 510 520 530 540 APQKIWEQLG IDSSLTTWES LETFGQIPGN TNVQKGQPLF PRLDVEEEVA HIKSLMGGSK 550 560 570 580 590 600 KPEEAPKDEK EESDEITIDD FSKVELRIAE VIDAEPVKKA DRLLKIQLDL GYEKRQVVSG 610 620 630 640 650 660 IAKYYSPSDL IGKKVICVTN LKPVKLRGEL SQGMILAGSE GDQLRLATVD GALPNGSLVK

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB03772.1.
PIR	E83656.
RefSeq	NP_240919.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KGK8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000052632; EBBACP00000051271; EBBACG00000052623.
GeneID	892709.
GenomeReviews	Gene locus BH0053 in contig BA000004_GR.
KEGG	bha:BH0053.
NMPDR	fig|272558.1.peg.53.
PATRIC	18936932. VBIBacHal18977_0056.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000031891.
HOGENOM	HBG721667.
OMA	FDWGIKV.
PhylomeDB	Q9KGK8.
ProtClustDB	PRK12267.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0053-MONOMER.
Family and domain databases
HAMAP	MF_01228. Met_tRNA_synth_type2. Fused. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR014758. Met-tRNA_synth. IPR023457. Met-tRNA_synth_2. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit. TIGR00399. MetG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BACHD

Accession

Primary (citable) accession number: Q9KGK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program