Q9KGJ6 (GLMU_BACHD) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 67.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Bifunctional protein GlmU | ||||
| Gene names |
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| Organism | Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 272558 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 455 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc By similarity. HAMAP MF_01631 |
| Catalytic activity | Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate. HAMAP MF_01631 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-D-glucosamine. HAMAP MF_01631 |
| Cofactor | Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01631 |
| Pathway | Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2. HAMAP MF_01631 Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_01631 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_01631. |
| Sequence similarities | In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family. In the C-terminal section; belongs to the transferase hexapeptide repeat family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 455 | 455 | Bifunctional protein GlmU HAMAP MF_01631 | PRO_0000233731 | |||||
Regions | |||||||||
| Region | 1 – 230 | 230 | Pyrophosphorylase By similarity | ||||||
| Region | 9 – 12 | 4 | Substrate binding By similarity | ||||||
| Region | 78 – 79 | 2 | Substrate binding By similarity | ||||||
| Region | 231 – 251 | 21 | Linker By similarity | ||||||
| Region | 252 – 455 | 204 | N-acetyltransferase By similarity | ||||||
Sites | |||||||||
| Active site | 363 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 103 | 1 | Magnesium By similarity | ||||||
| Metal binding | 228 | 1 | Magnesium By similarity | ||||||
| Binding site | 73 | 1 | Substrate By similarity | ||||||
| Binding site | 140 | 1 | Substrate; via amide nitrogen By similarity | ||||||
| Binding site | 155 | 1 | Substrate By similarity | ||||||
| Binding site | 170 | 1 | Substrate By similarity | ||||||
| Binding site | 387 | 1 | Acetyl-CoA By similarity | ||||||
| Binding site | 423 | 1 | Acetyl-CoA; via amide nitrogen By similarity | ||||||
| Binding site | 440 | 1 | Acetyl-CoA By similarity | ||||||
Sequences
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References
| [1] | "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BA000004 Genomic DNA. Translation: BAB03784.1. |
| PIR | A83658. |
| RefSeq | NP_240931.1. NC_002570.2. |
3D structure databases | |
| ProteinModelPortal | Q9KGJ6. |
| SMR | Q9KGJ6. Positions 3-452. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBACT00000051920; EBBACP00000050559; EBBACG00000051911. |
| GeneID | 892269. |
| GenomeReviews | Gene locus BH0065 in contig BA000004_GR. |
| KEGG | bha:BH0065. |
| NMPDR | fig|272558.1.peg.65. |
| PATRIC | 18936960. VBIBacHal18977_0070. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| GeneTree | EBGT00050000000560. |
| HOGENOM | HBG688195. |
| OMA | GSKVNHL. |
| PhylomeDB | Q9KGJ6. |
| ProtClustDB | CLSK2748798. |
Enzyme and pathway databases | |
| BioCyc | BHAL272558:BH0065-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01631. GlmU. [Tree] |
| InterPro | IPR005882. Bifunctional_GlmU. IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR005835. NTP_transferase. IPR011004. Trimer_LpxA-like. [Graphical view] |
| KO | K04042. |
| PANTHER | PTHR22572:SF17. PTHR22572:SF17. 1 hit. |
| Pfam | PF00132. Hexapep. 3 hits. PF00483. NTP_transferase. 1 hit. [Graphical view] |
| SUPFAM | SSF51161. Trimer_LpxA_like. 1 hit. |
| TIGRFAMs | TIGR01173. GlmU. 1 hit. |
| PROSITE | PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GLMU_BACHD | ||||||||
| Accession | Primary (citable) accession number: Q9KGJ6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with