Reviewed, UniProtKB/Swiss-Prot Q9KGJ5 (KPRS_BACHD)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Ordered Locus Names:	BH0066

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity HAMAP MF_00583.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141109
Regions
	Region	216 – 229	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	133	1	Magnesium Potential
	Metal binding	135	1	Magnesium Potential
	Metal binding	144	1	Magnesium Potential
	Metal binding	148	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9KGJ5-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C4B7D7FACF3C94B8	FASTA	316	34,521
	10 20 30 40 50 60 MANYGDPNLK VFTLNSNKGL AYEITERIGV PMGKSSVTRF SDGEVQINIE ESIRGCDVFL 70 80 90 100 110 120 IQSTAAPANE HIMELLIMID AVKRASAKTI NVVIPYYGYA RQDRKARARE PITAKLVANL 130 140 150 160 170 180 LETAGATRVL TLDLHATQIQ GFFDIPVDQL MGVPILAEFF EKKGLDDVVV VSPDHGGVVR 190 200 210 220 230 240 ARKLADRLKA PIAIIDKRRP KPNVAEVMNI VGHIEGKTAI IIDDIIDTAG TITLAANALV 250 260 270 280 290 300 EHGAKEVYAC CTHPVLSGPA IERIKNSKIK ELVVMNTIEL EEEKLIDKIT TLSVAPLMAE 310 AIVRVHEHSS VSLLFD

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB03785.1.
PIR	B83658.
RefSeq	NP_240932.1.
3D structure databases
SMR	Q9KGJ5. Positions 4-315.
ModBase	Search...
Genome annotation databases
GeneID	892693.
GenomeReviews	Gene locus BH0066 in contig BA000004_GR.
KEGG	bha:BH0066.
NMPDR	fig|272558.1.peg.66.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG519284.
OMA	CATHAVF.
PhylomeDB	Q9KGJ5.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0066-MONOMER.
BRENDA	2.7.6.1. 191865.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_BACHD

Accession

Primary (citable) accession number: Q9KGJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)