Q9KGF4 (SYC_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	BH0111

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000159348
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity
Amino acid modifications
	Modified residue	270	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KGF4 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E11C69C9C91DCF3C	FASTA	466	53,539
	10 20 30 40 50 60 MAIQVFNSLT RKKEPFVPLE EGKVKMYVCG PTVYNYIHIG NARPPIVYDM IRRYLKYRGY 70 80 90 100 110 120 EVLFVSNFTD VDDKIIRVAN ELGEDVFAVA NRFIEAYKQD TSALGVLEAD HHPRVTETMS 130 140 150 160 170 180 EIISFIRVLE EKGYAYEQGG DVYFRTRKFK EYGKLSAQSI DDLLSGARIE VDERKEDPLD 190 200 210 220 230 240 FVLWKAAKEG EVSWDSPWGK GRPGWHIECS AMVKKYLGDT IDIHAGGQDL KFPHHENEIA 250 260 270 280 290 300 QSECMTGKPM ANYWLHNGFI NLDNEKMSKS LGNVVLANEM IRQHSAEVLR FFMLSAHYRS 310 320 330 340 350 360 PINFSDELLE GAKRGLERLQ TAVSSLVHRL QESADFGGTE PWLERIDEFR GRFIEEMDDD 370 380 390 400 410 420 FNSANGIAVL FDLAKEANRY LREEQTSKAV LQSFIDLFNE LGGVLGVTLN RQEELLDEEI 430 440 450 460 ERLIEERNEA RKAKNFQRAD DIRDQLKAEG IILEDTPQGV RWKRGS

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB03830.1.
PIR	G83663.
RefSeq	NP_240977.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KGF4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000051516; EBBACP00000050155; EBBACG00000051507.
GeneID	892757.
GenomeReviews	Gene locus BH0111 in contig BA000004_GR.
KEGG	bha:BH0111.
NMPDR	fig|272558.1.peg.111.
PATRIC	18937096. VBIBacHal18977_0120.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000000801.
HOGENOM	HBG327651.
OMA	DFDALNM.
PhylomeDB	Q9KGF4.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0111-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_BACHD

Accession

Primary (citable) accession number: Q9KGF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program