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Reviewed, UniProtKB/Swiss-Prot Q9KG59 (URE1_BACHD)

Last modified November 3, 2009. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha
Gene names
Name: ureC
Ordered Locus Names: BH0254
OrganismBacillus halodurans [Complete proteome] [HAMAP]
Taxonomic identifier86665 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length571 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953

Cofactor

Binds 2 nickel ions per subunit By similarity.

Pathway

Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953

Subunit structure

Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamylation allows a single lysine to coordinate two nickel ions By similarity.

Sequence similarities

Belongs to the urease family.

Contains 1 urease domain.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Nickel
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processurea metabolic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionnickel ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

urease activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 571571Urease subunit alpha HAMAP MF_01953
PRO_0000234133

Regions

Domain132 – 571440Urease

Sites

Active site3231Proton donor By similarity
Metal binding1371Nickel 2 By similarity
Metal binding1391Nickel 2 By similarity
Metal binding2201Nickel 1; via carbamate group By similarity
Metal binding2201Nickel 2; via carbamate group By similarity
Metal binding2491Nickel 1 By similarity
Metal binding2751Nickel 1 By similarity
Metal binding3631Nickel 2 By similarity
Binding site2221Substrate By similarity

Amino acid modifications

Modified residue2201N6-carboxylysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9KG59-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 397398DECEA0AB0F

FASTA57161,292
        10         20         30         40         50         60 
MKLTRAQHAS LYGPTVGDKV RLADTDLLLE IEKDYTVYGD ECKFGGGKVL RDGMGQSAVY 

        70         80         90        100        110        120 
TRDEGVLDLI ITNATIIDYT GIVKADIGIK DGHIVGIGKG GNPDIMDGVE SHMIVGASTE 

       130        140        150        160        170        180 
AIAGEGLIVT AGGIDAHIHF ISPQQIDVAI ASGITTMLGG GTGPATGTKA TTCTPGKWNI 

       190        200        210        220        230        240 
ERMLEAADAF PVNLGFLGKG NASTPAPLRE QIEAGAIGLK LHEDWGTTPA AIRTCLSVAD 

       250        260        270        280        290        300 
RMDVQVAIHT DTLNEAGFVE DTIKAIGDRV IHTYHTEGAG GGHAPDIMKV AGLPNVLPSS 

       310        320        330        340        350        360 
TNPTRPFTVN TIDEHLDMLM VCHHLDPNVP EDVAFADSRI RPETIAAEDI LQDLGVISMI 

       370        380        390        400        410        420 
SSDSQAMGRV GEVIIRTWQT ADKMKKQRGA LAEDQGKGND NVRIKRYVSK YTINPAITHG 

       430        440        450        460        470        480 
IDDYVGSVEV GKLADLVLWD PRFFGVKPEL ILKGGLIAYS QMGDPNASIP TPQPVFSRPM 

       490        500        510        520        530        540 
FGSFGRARGT TSITFLSKAA MDLGVHEALG LQKKIAHVKN CRSISKRSMK YNDATPNIEI 

       550        560        570 
DPETYEVKVD GEMITCKPFE EVALAQRYFL F

« Hide

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References

[1]"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

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Cross-references

Sequence databases

BA000004 Genomic DNA. Translation: BAB03973.1.
PIRF83681.
RefSeqNP_241120.1.

3D structure databases

HSSPHSSP built from PDB template 1UBP based on UniProtKB P41020.
SMRQ9KG59. Positions 1-571.
ModBaseSearch...

Protein family/group databases

MEROPSM38.982.

Genome annotation databases

GeneID892359.
GenomeReviewsGene locus BH0254 in contig BA000004_GR.
KEGGbha:BH0254.
NMPDRfig|272558.1.peg.254.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ9KG59.
OMASHIHFIC.

Enzyme and pathway databases

BioCycBHAL272558:BH0254-MON.
BRENDA3.5.1.5. 191865.

Family and domain databases

HAMAPMF_01953.
[Tree]
InterProIPR006680. Amidohydro_1.
IPR011612. Urease_alpha_N.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR017952. Urease_asu_core.
IPR017950. Urease_asu_CS.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSPR01752. UREASE.
ProDomPD001248. Amidohydro_like. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01792. urease_alph. 1 hit.
PROSITEPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameURE1_BACHD
AccessionPrimary (citable) accession number: Q9KG59
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: October 1, 2000
Last modified: November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents