Q9KG45 (GLMS_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucosamine--fructose-6-phosphate aminotransferase [isomerizing]
EC=2.6.1.16

Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine-D-fructose-6-phosphate amidotransferase

Gene names

Name:	glmS
Ordered Locus Names:	BH0268

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source By similarity. HAMAP MF_00164
Catalytic activity	L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate. HAMAP MF_00164
Subunit structure	Homodimer By similarity. HAMAP MF_00164
Subcellular location	Cytoplasm By similarity HAMAP MF_00164.
Sequence similarities	Contains 1 glutamine amidotransferase type-2 domain. Contains 2 SIS domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase Repeat
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 600	599	Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] HAMAP MF_00164		PRO_0000135296
Regions
	Domain	2 – 217	216	Glutamine amidotransferase type-2
	Domain	283 – 422	140	SIS 1
	Domain	452 – 590	139	SIS 2
Sites
	Active site	2	1	Nucleophile; for GATase activity By similarity
	Active site	595	1	For Fru-6P isomerization activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KG45 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5EE30645E84DA0EC	FASTA	600	65,877
	10 20 30 40 50 60 MCGIVGYIGT EDAKEILLKG LEKLEYRGYD SAGIAVATEE GVHIFKEKGR IATLREVVDQ 70 80 90 100 110 120 GVKSTVGIGH TRWATHGEPS KVNAHPHQSE SSRFTIVHNG VIENYEQLKR DYLQAVTLQS 130 140 150 160 170 180 DTDTEVVVQL VERFVHEGQS VEAAFRHTLS LLKGSYAIAL LDKKDTDTIY VGKNKSPLLV 190 200 210 220 230 240 GVQDGVNVVA SDAMAMLQVT DQFLEIMDEE IVIVTKDDVT IKTLDGTAVS REPYTAELDA 250 260 270 280 290 300 SDIEKGTYPH FMLKEIDEQP FVIRNIIQKY QNEDGSVRLD EDIRQAVMEA DRIYIIAAGT 310 320 330 340 350 360 SYHAGLVGKQ LIENFAKQPV EVHIASEFLY NMPLLSKRPL FIFISQSGET ADSRGVLVEV 370 380 390 400 410 420 KKLGYKALTI TNVPGSTLSR EADFTLHTHA GPEIAVASTK AYTAQMAVLT LLAADTAKAK 430 440 450 460 470 480 GIDLGFDPIQ ELSIVANAME TLCNQKETLE KIARDYLAVT RNCFFIGRAT DYFVCLEGAL 490 500 510 520 530 540 KLKEISYIQA EGFAGGELKH GTIALIEEGT PIVALATQEH VNLSIRGNVK EVAARGAFPC 550 560 570 580 590 600 IISMEGLNEE DDTFVLPKVH SHLTPLVSVI PLQLIAYYAA LHRGCDVDKP RNLAKSVTVE

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB03987.1.
PIR	D83683.
RefSeq	NP_241134.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KG45.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000050711; EBBACP00000049350; EBBACG00000050702.
GeneID	892397.
GenomeReviews	Gene locus BH0268 in contig BA000004_GR.
KEGG	bha:BH0268.
NMPDR	fig|272558.1.peg.268.
PATRIC	18937448. VBIBacHal18977_0283.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000000004.
HOGENOM	HBG645312.
OMA	LCASQES.
ProtClustDB	PRK00331.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0268-MONOMER.
Family and domain databases
HAMAP	MF_00164. GlmS. [Tree]
InterPro	IPR000583. GATase_2. IPR017932. GATase_II. IPR005855. GlmS_trans. IPR001347. SIS. [Graphical view]
KO	K00820.
Pfam	PF00310. GATase_2. 2 hits. PF01380. SIS. 2 hits. [Graphical view]
TIGRFAMs	TIGR01135. GlmS. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS51464. SIS. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLMS_BACHD

Accession

Primary (citable) accession number: Q9KG45

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program