Alpha-amylase G-6 - Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

Preferred order of sections

Names and origin

Protein names

Submitted name:
Alpha-amylase G-6 EMBL BAB04132.1

Gene names

Ordered Locus Names:

BH0413

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	958 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 2C3H PDB 2C3W PDB 2C3X PDB 2C3V PDB 2C3G Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	catalytic activity Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

788 – 790

3

Glucose binding PDB 2C3H

Region

841 – 842

2

Glucose binding PDB 2C3H

Region

872 – 876

5

Glucose binding PDB 2C3W

Region

930 – 937

8

Glucose binding PDB 2C3W PDB 2C3X

Sites

Binding site

801

1

Glucose PDB 2C3H

Binding site

836

1

Glucose PDB 2C3H

Binding site

882

1

Glucose PDB 2C3W PDB 2C3X

Binding site

890

1

Glucose PDB 2C3W PDB 2C3X

Binding site

949

1

Glucose PDB 2C3W

Sequences

Sequence

Length

Mass (Da)

Tools

Q9KFR4 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: C366087A167F024E
Show »

FASTA

958

107,162

        10         20         30         40         50         60 
MNASYVKKVS LYLLLSFVIT LPLFSPTFSI NSVDASQGGE QQSFSWDNAT VYFAITDRFH 

        70         80         90        100        110        120 
DGNPSNNQSY GRPQEDAWGQ NIGTFHGGDL QGLTDKLNEG YFTELGINAI WITAPYEQVH 

       130        140        150        160        170        180 
GWVGGGSDGD FAHYAYHGYY ALDYTMIDQN MGTVEDMREF VHTAHEQGIR VVLDVVMNHP 

       190        200        210        220        230        240 
GYNTIKDMHE YGFGNPGVSE HWTPGQGQNW HDVHQQINYE DAGAWRHWWG PWIRAGIAGY 

       250        260        270        280        290        300 
ESCGNSEITM CLAGLPDFRT ELTHSVGLPP LLETKWNQER QGGYEDWIVP AANDLRRDLG 

       310        320        330        340        350        360 
VAPADYIVKW LSAWVEEFGI DGFRVDTAKH VELSRWQQLK DAANEALWKW REENPEAPGA 

       370        380        390        400        410        420 
NWTDDFWMVG EVWGHGVGRS EYFDHGFDSV INFTFQGEHG NGPAYRLDTM ESTFSRYANA 

       430        440        450        460        470        480 
INTDSTFNVL SYLSQHDTQL YPRERLIDGG TYLMLLPGGV QVFYGDETAR PFGPTGSDPH 

       490        500        510        520        530        540 
QGTRSSMNWD SVNEEVLHHW QKMGQFRSNH LAVGAGEHRQ IAASPYTFSR TYVEGDVDDR 

       550        560        570        580        590        600 
VVVVVGASGE TTIDVSSVFS DGQQVRDFYT GNVTVVENGH VTFPAHRNGV ILIERAGDLL 

       610        620        630        640        650        660 
PSVSASPAGG EFDREQQEVI LYVQHAEWGA YTIDGSDPKE EGTAFANGDV ITVGRELNID 

       670        680        690        700        710        720 
ESLTLRLYAE NETGEAKAEF TFTKVPAYPQ VFADPPGGTF AGDTLKVTLH TRNVEGGTYS 

       730        740        750        760        770        780 
IDGSDEQAFT DGDEIMIGKG IEAGSETTLQ LKAENEFGTI EETYTYTKST GLTIYFKKPD 

       790        800        810        820        830        840 
SWGTPHLYYY DTNPKVDEPT WSEAPEMEHY EGDWYTHTIE GVESVRLLFK DRGTNQWPGP 

       850        860        870        880        890        900 
GEPGFFRDQD GWFDGEWHVD RPGDATDITI YYKTGWTHPH IHYSLNQGAW TTLPGVPLTK 

       910        920        930        940        950 
SEYEGYVKVT IEAEEGSQLR AAFNNGSGQW DNNQGRDYDF SSGVHTLADG RILSGTPK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
[2]	"A structural and functional analysis of alpha-glucan recognition by family 25 and 26 carbohydrate-binding modules reveals a conserved mode of starch recognition." Boraston A.B., Healey M., Klassen J., Ficko-Blean E., Lammerts van Bueren A., Law V. J. Biol. Chem. 281:587-598(2006) [PubMed: 16230347] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.39 ANGSTROMS) OF 863-958 IN COMPLEX WITH GLUCOSE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000004 Genomic DNA. Translation: BAB04132.1.

PIR

E83701.

RefSeq

NP_241279.1. NC_002570.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2C3G	X-ray	2.00	A	771-863	[»]
2C3H	X-ray	2.24	A/B/C/D/E/F/G/H	771-863	[»]
2C3V	X-ray	1.39	A/B	863-958	[»]
2C3W	X-ray	1.81	A/B/C/D	863-958	[»]
2C3X	X-ray	2.70	A/B	863-958	[»]

ProteinModelPortal

Q9KFR4.

SMR

Q9KFR4. Positions 771-862, 864-958.

ModBase

Search...

Protein family/group databases

CAZy

CBM25. Carbohydrate-Binding Module Family 25.
CBM26. Carbohydrate-Binding Module Family 26.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000052695; EBBACP00000051334; EBBACG00000052686.

GeneID

892065.

GenomeReviews

Gene locus BH0413 in contig BA000004_GR.

KEGG

bha:BH0413.

NMPDR

fig|272558.1.peg.413.

PATRIC

18937748. VBIBacHal18977_0433.

Organism-specific databases

CMR

Search...

Phylogenomic databases

GeneTree

EBGT00070000031973.

HOGENOM

HBG345590.

OMA

TELTHSV.

PhylomeDB

Q9KFR4.

ProtClustDB

CLSK2565252.

Family and domain databases

InterPro

IPR015902. Alpha_amylase.
IPR005085. CBM_fam25.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.

KO

K01176.

PANTHER

PTHR10357. Alpha_amylase. 1 hit.

Pfam

PF00128. Alpha-amylase. 1 hit.
PF03423. CBM_25. 1 hit.
[Graphical view]

SMART

SM00642. Aamy. 1 hit.
SM01066. CBM_25. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q9KFR4_BACHD

Accession

Primary (citable) accession number: Q9KFR4

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2000
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)