Q9KFQ8 (NAGB_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucosamine-6-phosphate deaminase
EC=3.5.99.6

Alternative name(s):
GlcN6P deaminase
Short name=GNPDA
Glucosamine-6-phosphate isomerase

Gene names

Name:	nagB
Ordered Locus Names:	BH0420

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion By similarity. HAMAP MF_01241
Catalytic activity	D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3. HAMAP MF_01241
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 5/5. HAMAP MF_01241
Sequence similarities	Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glucosamine-6-phosphate deaminase activity Inferred from electronic annotation. Source: EC hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Glucosamine-6-phosphate deaminase HAMAP MF_01241		PRO_0000160131
Sites
	Active site	67	1	Proton acceptor; for enolization step By similarity
	Active site	136	1	For ring-opening step By similarity
	Active site	138	1	Proton acceptor; for ring-opening step By similarity
	Active site	143	1	For ring-opening step By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KFQ8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 640E25631C9EC6E6	FASTA	246	27,520
	10 20 30 40 50 60 MKIIEAKHFD DMSLKAAQFM IEKIQRDPTI TLGLATGGTP QKMYELLIND HRTNGTSYHQ 70 80 90 100 110 120 VTTFNLDEYI GLDRHDPNSY YTYMHKALFD HIDIRDEQAF LPNGTASNFN AECERYEALI 130 140 150 160 170 180 QQRGGIDLQV LGIGANGHIG FNEPGTSFES STHIVKLTDS TREANARYFN DLSEVPTEAI 190 200 210 220 230 240 TMGIQSIMNA KEILLLASGK KKADALYQLI HGKVDESFPA SVLQRHEQVT IIADREALQK VAVSSN

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04139.1.
PIR	D83702.
RefSeq	NP_241286.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KFQ8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000052809; EBBACP00000051448; EBBACG00000052800.
GeneID	894561.
GenomeReviews	Gene locus BH0420 in contig BA000004_GR.
KEGG	bha:BH0420.
NMPDR	fig|272558.1.peg.420.
PATRIC	18937762. VBIBacHal18977_0440.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002591.
HOGENOM	HBG725991.
OMA	ECRRYEQ.
PhylomeDB	Q9KFQ8.
ProtClustDB	CLSK2748844.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0420-MONOMER.
Family and domain databases
HAMAP	MF_01241. GlcN6P_deamin. [Tree]
InterPro	IPR006148. Glc/Gal-6P_isomerase. IPR004547. Glucosamine6P_isomerase. IPR018321. Glucosamine6P_isomerase_CS. [Graphical view]
KO	K02564.
PANTHER	PTHR11280. NagB. 1 hit.
Pfam	PF01182. Glucosamine_iso. 1 hit. [Graphical view]
TIGRFAMs	TIGR00502. NagB. 1 hit.
PROSITE	PS01161. GLC_GALNAC_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGB_BACHD

Accession

Primary (citable) accession number: Q9KFQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program