Q9KFP1 (METE_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	BH0438

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098612
Sites
	Metal binding	639	1	Zinc By similarity
	Metal binding	641	1	Zinc By similarity
	Metal binding	724	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KFP1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 6F7FA9352A42AE6A	FASTA	756	85,157
	10 20 30 40 50 60 MLTSNLGYPR IGENREWKKA LESFWANDTT EEQLLATMKE LRLNHLRVQQ EQEVDLIPVG 70 80 90 100 110 120 DFTLYDHVLD MAVMFGIIPK RFLQQGDTPT LSTYFAMARG SKNAQACEMT KWYNTNYHYI 130 140 150 160 170 180 VPELHDAAPR LTKNAPLEAY LEAKNELGID GKPVILGPYS FVKLAKGYEE DKLQETIQSL 190 200 210 220 230 240 LPLYIQVIQE LVDAGARSIQ VDEPSLVTSI SAREMALVTR IYEQINEAIA DAPLFLQTYF 250 260 270 280 290 300 DAVTFYEEVV SLPVKGIGLD FVHGGAKNLE ALRTFGFPED KVLAAGIIDG RNIWISNLRE 310 320 330 340 350 360 RHELVHQLEQ HVAKDRLVLQ PSCSLLHVPV TTKREEKLDP TLLGVLAFAN EKLTELHTLK 370 380 390 400 410 420 QLAAGNEAEV KEALEANDDA LAALEKSGWR SGAATSHNLE NKKRPQSFNE RRPLQEEKWQ 430 440 450 460 470 480 LPLLPTTTIG SFPQTKDVRR TRSLWRKGEL STVEYERTMK SYIEKWINIQ EELGLDVLVH 490 500 510 520 530 540 GEFERNDMVE FFGEKLDGFA FTANGWVQSY GSRCVKPPII YGNVSFTEPM TVAETVYAQS 550 560 570 580 590 600 LTDKPVKGML TGPVTILNWS FVRDDLPLTV IAHQIAEALT HEVTALEEAG IEMIQIDEPA 610 620 630 640 650 660 IREGLPLKAE DQQEYLDWAV SAFRASCAHV KATTQIHTHM CYSEFHEMIE AIDDLDADVI 670 680 690 700 710 720 SIETSRSHGE MISAFEKTTY EKGIGLGVYD IHSPRVPSEE EMLNVIRRAL TVLPASLFWV 730 740 750 NPDCGLKTRA EKETVAALKN MVAAARAARE ELKTKA

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04157.1.
PIR	F83704.
RefSeq	NP_241304.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KFP1.
SMR	Q9KFP1. Positions 1-751.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000052682; EBBACP00000051321; EBBACG00000052673.
GeneID	893412.
GenomeReviews	Gene locus BH0438 in contig BA000004_GR.
KEGG	bha:BH0438.
NMPDR	fig|272558.1.peg.438.
PATRIC	18937808. VBIBacHal18977_0463.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001686.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	Q9KFP1.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0438-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACHD

Accession

Primary (citable) accession number: Q9KFP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program