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Q9KFH7

- RIR2_BACHD

UniProt

Q9KFH7 - RIR2_BACHD

Protein

Ribonucleoside-diphosphate reductase subunit beta

Gene

nrdB

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi88 – 881Iron 1By similarity
    Metal bindingi118 – 1181Iron 1By similarity
    Metal bindingi118 – 1181Iron 2By similarity
    Metal bindingi121 – 1211Iron 1By similarity
    Active sitei125 – 1251By similarity
    Metal bindingi185 – 1851Iron 2By similarity
    Metal bindingi219 – 2191Iron 2By similarity
    Metal bindingi222 – 2221Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. deoxyribonucleotide biosynthetic process Source: InterPro
    3. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciBHAL272558:GJC5-539-MONOMER.
    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    Gene namesi
    Name:nrdB
    Ordered Locus Names:BH0502
    OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
    Taxonomic identifieri272558 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001258: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. ribonucleoside-diphosphate reductase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 345345Ribonucleoside-diphosphate reductase subunit betaPRO_0000190468Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.By similarity

    Protein-protein interaction databases

    STRINGi272558.BH0502.

    Structurei

    Secondary structure

    1
    345
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi21 – 233
    Helixi40 – 4910
    Helixi54 – 563
    Helixi60 – 656
    Helixi66 – 683
    Helixi71 – 8616
    Turni87 – 904
    Helixi94 – 996
    Helixi105 – 13228
    Helixi135 – 14612
    Helixi149 – 16719
    Helixi171 – 18616
    Turni187 – 1893
    Helixi190 – 20112
    Helixi206 – 23631
    Helixi238 – 2403
    Helixi243 – 26725
    Turni268 – 2703
    Helixi277 – 29418

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2RCCX-ray1.90A/B/C1-345[»]
    ProteinModelPortaliQ9KFH7.
    SMRiQ9KFH7. Positions 1-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9KFH7.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0208.
    KOiK00526.
    OMAiSEIFNAY.
    OrthoDBiEOG6J48J7.

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR026023. RNR_bsu_prok.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000355. NrdB. 1 hit.
    SUPFAMiSSF47240. SSF47240. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9KFH7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQLQKRKIY DTTASNASTG ILNGKSSNVL NWDDVRFSWA YPLYKNMLAN    50
    FWTPFEINMS HDAKQFPTLT ETEQEAFKKI IGLLAFLDSV QTDYSMRAAE 100
    YLTDSSLAAL MSVLSFQEVV HNQSYSYVLS SLVPKATQDE IFEYWKHDDV 150
    LKERNEFIID GYEKFVDNPT PKTFLESIVY DVILEGLNFY SGFAFFYNLA 200
    RNQKMVSTST MINYINRDEQ LHVYLFTNIF KELLVEFPEL NTEETKTFVK 250
    TTLMKAADLE KDWFRYIIGD KIPGINPEDM ETYISFIANK RAVQLGMEKP 300
    YPEIKHNPMK WIRAYEDVNS GKSDFFEQKS RQYAKVSADN GFDEL 345
    Length:345
    Mass (Da):40,242
    Last modified:October 1, 2000 - v1
    Checksum:iD89208F7518152BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000004 Genomic DNA. Translation: BAB04221.1.
    PIRiF83712.
    RefSeqiNP_241368.1. NC_002570.2.
    WP_010896680.1. NC_002570.2.

    Genome annotation databases

    EnsemblBacteriaiBAB04221; BAB04221; BAB04221.
    GeneIDi893380.
    KEGGibha:BH0502.
    PATRICi18937944. VBIBacHal18977_0531.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000004 Genomic DNA. Translation: BAB04221.1 .
    PIRi F83712.
    RefSeqi NP_241368.1. NC_002570.2.
    WP_010896680.1. NC_002570.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2RCC X-ray 1.90 A/B/C 1-345 [» ]
    ProteinModelPortali Q9KFH7.
    SMRi Q9KFH7. Positions 1-312.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272558.BH0502.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB04221 ; BAB04221 ; BAB04221 .
    GeneIDi 893380.
    KEGGi bha:BH0502.
    PATRICi 18937944. VBIBacHal18977_0531.

    Phylogenomic databases

    eggNOGi COG0208.
    KOi K00526.
    OMAi SEIFNAY.
    OrthoDBi EOG6J48J7.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .
    BioCyci BHAL272558:GJC5-539-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q9KFH7.

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR026023. RNR_bsu_prok.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000355. NrdB. 1 hit.
    SUPFAMi SSF47240. SSF47240. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
      Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
      Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

    Entry informationi

    Entry nameiRIR2_BACHD
    AccessioniPrimary (citable) accession number: Q9KFH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3