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Q9KFH7

- RIR2_BACHD

UniProt

Q9KFH7 - RIR2_BACHD

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Protein
Ribonucleoside-diphosphate reductase subunit beta
Gene
nrdB, BH0502
Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Iron 1 By similarity
Metal bindingi118 – 1181Iron 1 By similarity
Metal bindingi118 – 1181Iron 2 By similarity
Metal bindingi121 – 1211Iron 1 By similarity
Active sitei125 – 1251 By similarity
Metal bindingi185 – 1851Iron 2 By similarity
Metal bindingi219 – 2191Iron 2 By similarity
Metal bindingi222 – 2221Iron 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
  2. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  3. deoxyribonucleotide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-539-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:nrdB
Ordered Locus Names:BH0502
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001258: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Ribonucleoside-diphosphate reductase subunit beta
PRO_0000190468Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi272558.BH0502.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi21 – 233
Helixi40 – 4910
Helixi54 – 563
Helixi60 – 656
Helixi66 – 683
Helixi71 – 8616
Turni87 – 904
Helixi94 – 996
Helixi105 – 13228
Helixi135 – 14612
Helixi149 – 16719
Helixi171 – 18616
Turni187 – 1893
Helixi190 – 20112
Helixi206 – 23631
Helixi238 – 2403
Helixi243 – 26725
Turni268 – 2703
Helixi277 – 29418

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RCCX-ray1.90A/B/C1-345[»]
ProteinModelPortaliQ9KFH7.
SMRiQ9KFH7. Positions 1-312.

Miscellaneous databases

EvolutionaryTraceiQ9KFH7.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
KOiK00526.
OMAiSEIFNAY.
OrthoDBiEOG6J48J7.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026023. RNR_bsu_prok.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
PIRSFiPIRSF000355. NrdB. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KFH7-1 [UniParc]FASTAAdd to Basket

« Hide

MEQLQKRKIY DTTASNASTG ILNGKSSNVL NWDDVRFSWA YPLYKNMLAN    50
FWTPFEINMS HDAKQFPTLT ETEQEAFKKI IGLLAFLDSV QTDYSMRAAE 100
YLTDSSLAAL MSVLSFQEVV HNQSYSYVLS SLVPKATQDE IFEYWKHDDV 150
LKERNEFIID GYEKFVDNPT PKTFLESIVY DVILEGLNFY SGFAFFYNLA 200
RNQKMVSTST MINYINRDEQ LHVYLFTNIF KELLVEFPEL NTEETKTFVK 250
TTLMKAADLE KDWFRYIIGD KIPGINPEDM ETYISFIANK RAVQLGMEKP 300
YPEIKHNPMK WIRAYEDVNS GKSDFFEQKS RQYAKVSADN GFDEL 345
Length:345
Mass (Da):40,242
Last modified:October 1, 2000 - v1
Checksum:iD89208F7518152BC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000004 Genomic DNA. Translation: BAB04221.1.
PIRiF83712.
RefSeqiNP_241368.1. NC_002570.2.
WP_010896680.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB04221; BAB04221; BAB04221.
GeneIDi893380.
KEGGibha:BH0502.
PATRICi18937944. VBIBacHal18977_0531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000004 Genomic DNA. Translation: BAB04221.1 .
PIRi F83712.
RefSeqi NP_241368.1. NC_002570.2.
WP_010896680.1. NC_002570.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2RCC X-ray 1.90 A/B/C 1-345 [» ]
ProteinModelPortali Q9KFH7.
SMRi Q9KFH7. Positions 1-312.
ModBasei Search...

Protein-protein interaction databases

STRINGi 272558.BH0502.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB04221 ; BAB04221 ; BAB04221 .
GeneIDi 893380.
KEGGi bha:BH0502.
PATRICi 18937944. VBIBacHal18977_0531.

Phylogenomic databases

eggNOGi COG0208.
KOi K00526.
OMAi SEIFNAY.
OrthoDBi EOG6J48J7.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci BHAL272558:GJC5-539-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q9KFH7.

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026023. RNR_bsu_prok.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
PIRSFi PIRSF000355. NrdB. 1 hit.
SUPFAMi SSF47240. SSF47240. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiRIR2_BACHD
AccessioniPrimary (citable) accession number: Q9KFH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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