Reviewed, UniProtKB/Swiss-Prot Q9KFF9 (ALR_BACHD)

Names and origin

Protein names

Recommended name:
    Alanine racemase
    EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	BH0520

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Alanine racemase HAMAP MF_01201		PRO_0000114497
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	269	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KFF9-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8017C15D03C9A66A	FASTA	388	44,021
	10 20 30 40 50 60 MEHFYRDTWV EVDLDAIEQN VTNALRLYKD REMNLMAVVK ANGYGHGAVE VSQAALRAGA 70 80 90 100 110 120 SYLSVAFLDE ALALRKAGID APILVMGLVG AEHVQLAIKH RITLTVYQLD WLEQAISRLH 130 140 150 160 170 180 TRDKLAIHLK LDTGMGRIGL RRKEDIRSCM DFIASHECFE LEGVFTHFAT ADEKDLTYFK 190 200 210 220 230 240 KQCQRFNQWL EYIREWQLPI RYVHCGNSAA GLRFPEKNFN MFRFGIAMYG LTPSPEITDE 250 260 270 280 290 300 LPFPLKQAFS LKSRLSNVKK LPKGEGISYG ATYVTEGAEW IGTLPIGYAD GWIRHHSNAG 310 320 330 340 350 360 GHVLIDGKRA PFVGRICMDQ CMIRLPKKHS IGDTVTLIGE SDGKQITMDE VAKRLNTINY 370 380 EIPCVISWRV PRIYFRDGQI ISVKNNLL

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04239.1.
PIR	H83714.
RefSeq	NP_241386.1.
3D structure databases
SMR	Q9KFF9. Positions 3-387.
ModBase	Search...
Genome annotation databases
GeneID	893379.
GenomeReviews	Gene locus BH0520 in contig BA000004_GR.
KEGG	bha:BH0520.
NMPDR	fig|272558.1.peg.520.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG712172.
OMA	FIASHEC.
PhylomeDB	Q9KFF9.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0520-MONOMER.
BRENDA	5.1.1.1. 191865.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_BACHD

Accession

Primary (citable) accession number: Q9KFF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)