Reviewed, UniProtKB/Swiss-Prot Q9KFD3 (GCP_BACHD)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BH0548

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303267
Sites
	Metal binding	120	1	Zinc Potential
	Metal binding	124	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9KFD3-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 09AD4FD67BD0C18C	FASTA	343	36,708
	10 20 30 40 50 60 MGDVVMNERE LILAIETSCD ETSAAVIENG TTILSNVVSS QIDSHKRFGG VVPEIASRHH 70 80 90 100 110 120 VEQITVIVEE AMHEAGVDFA DLAAVAVTEG PGLVGALLIG VNAAKAIAFA HQLPLIGVHH 130 140 150 160 170 180 IAGHIYANRL LKELEFPLLA LVVSGGHTEL IYMENHGEFE VIGETRDDAV GEAYDKVART 190 200 210 220 230 240 LGLPYPGGPH IDRLAVNGED TLQFPRAWLE PDSFDFSFSG LKSAVINTLH NAKQRGENVQ 250 260 270 280 290 300 AEDVAASFQA SVIDVLVTKT KKAAEEYKVR QVLLAGGVAA NKGLRTALEE AFFKEPIDLV 310 320 330 340 IPPLSLCTDN AAMIGAAASI KFKQQTFAGM DLNGQPSLEL END

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB04267.1.
PIR	D83718.
RefSeq	NP_241414.2.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	893283.
GenomeReviews	Gene locus BH0548 in contig BA000004_GR.
KEGG	bha:BH0548.
NMPDR	fig|272558.1.peg.548.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9KFD3.
OMA	CKRALKQ.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0548-MON.
BRENDA	3.4.24.57. 191865.
Family and domain databases
HAMAP	MF_01445. [Tree]
InterPro	IPR009180. Pept_M22_Osialgl. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
ProDom	PD002367. Peptidase_M22. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BACHD

Accession

Primary (citable) accession number: Q9KFD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)