Q9KF75 (CYSI_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfite reductase [NADPH] hemoprotein beta-component
Short name=SiR-HP
Short name=SiRHP
EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	BH0610

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP MF_01540
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. HAMAP MF_01540 Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP MF_01540
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000388475
Sites
	Metal binding	437	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	443	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	482	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	486	1	Iron (siroheme axial ligand) By similarity
	Metal binding	486	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KF75 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7B9C545068D34C8D	FASTA	572	65,004
	10 20 30 40 50 60 MGNERLKAPE GPPSDVERIK EESNYLRGTL KESMLEPISA GISDDDNRLM KHHGSYLQDD 70 80 90 100 110 120 RDLRNERQKQ KLEPAYQFML RVRMPGGVAT PEQWLVMDEL AQKYGNGTMK LTTRETFQLH 130 140 150 160 170 180 GILKWNMKKT IQAIHSALLD TIAACGDVNR NVMCTSNPYQ SEIHQEVYEW SKKLSDDLLP 190 200 210 220 230 240 RTRAYHEIWL DEEKVAGTPE AEEVEPMYGP LYLPRKFKIG IAVPPSNDID VFSQDLGFIA 250 260 270 280 290 300 IVEDDKLVGF NVAIGGGMGM THGDKATYPQ LAKVIGFCKP EQIYDVAEKI ITIQRDYGNR 310 320 330 340 350 360 SVRKNARFKY TVDRLGLETV KNELENRLGW SLDEAKPYHF DHNGDRYGWV KGVKGRWHFT 370 380 390 400 410 420 LFVEGGRVTD YADYKLMTGL REIAKIHTGD IRLTSNQNLI IANVTSQKKK KINELIEQYG 430 440 450 460 470 480 LTDGKHYTAL RRSSIACVAL PTCGLAMAEA ERYLPKLIDK IDEIIEENGL KNEEITIRMT 490 500 510 520 530 540 GCPNGCARHA LGEIGFIGKA PGKYNMYLGA AFDGSRLSKM YRENIGEEEI LKELREILPR 550 560 570 YAKERKEGEH FGDFVIRAGI IKETTDGTNF HD

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04329.1.
PIR	B83726.
RefSeq	NP_241476.1. NC_002570.2.
3D structure databases
HSSP	HSSP built from PDB template 7GEP based on UniProtKB P17846.
ProteinModelPortal	Q9KF75.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000050948; EBBACP00000049587; EBBACG00000050939.
GeneID	893170.
GenomeReviews	Gene locus BH0610 in contig BA000004_GR.
KEGG	bha:BH0610.
PATRIC	18938196. VBIBacHal18977_0641.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002740.
HOGENOM	HBG626671.
OMA	RPYHFEH.
PhylomeDB	Q9KF75.
ProtClustDB	PRK13504.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0610-MONOMER.
Family and domain databases
HAMAP	MF_01540. CysI. [Tree]
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Gene3D	G3DSA:3.90.480.10. G3DSA:3.90.480.10. 2 hits.
KO	K00381.
Pfam	PF01077. NIR_SIR. 2 hits. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
SUPFAM	SSF55124. NiR_SiRalpha_1/3. 2 hits.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_BACHD

Accession

Primary (citable) accession number: Q9KF75

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program