Q9KF58 (PURQ_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 1
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase I
Short name=FGAM synthase I

Gene names

Name:	purQ
Ordered Locus Names:	BH0628

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00421
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00421
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00421.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Phosphoribosylformylglycinamidine synthase 1 HAMAP MF_00421		PRO_0000100535
Regions
	Domain	3 – 225	223	Glutamine amidotransferase type-1
Sites
	Active site	86	1	Nucleophile By similarity
	Active site	194	1	By similarity
	Active site	196	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KF58 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 65F2C8861827BE5D	FASTA	227	25,161
	10 20 30 40 50 60 MKFAVIVFPG SNCDADMYHA VKDALGEEVD YLWHTETSVE GYDAILLPGG FSYGDYLRSG 70 80 90 100 110 120 SIARFSPIME DVIRAANEGV PVLGVCNGFQ VLLEAGLLPG AMLRNEKLTF ICKPVELEVQ 130 140 150 160 170 180 NNDTFFTSEY EQGETITIPV AHGEGNYYCD DETLKQLEAN NQVVFRYTDR VNGSRNHIAG 190 200 210 220 IVNKAGNVLG MMPHPERAVE QLVGGEDGLR LFKSILRNWR ESHVVTP

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04347.1.
PIR	D83728.
RefSeq	NP_241494.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KF58.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000052686; EBBACP00000051325; EBBACG00000052677.
GeneID	893142.
GenomeReviews	Gene locus BH0628 in contig BA000004_GR.
KEGG	bha:BH0628.
NMPDR	fig|272558.1.peg.628.
PATRIC	18938246. VBIBacHal18977_0661.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001675.
HOGENOM	HBG302712.
OMA	FPGTNCD.
PhylomeDB	Q9KF58.
ProtClustDB	PRK03619.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0628-MONOMER.
Family and domain databases
HAMAP	MF_00421. PurQ. [Tree]
InterPro	IPR017926. GATASE_1. IPR010075. PRibForGlyAmidine_synth_I. [Graphical view]
KO	K01952.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PIRSF	PIRSF001586. FGAM_synth_I. 1 hit.
TIGRFAMs	TIGR01737. FGAM_synth_I. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURQ_BACHD

Accession

Primary (citable) accession number: Q9KF58

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program