Q9KF57 (PURL_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	BH0629

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100436
Regions
	Nucleotide binding	111 – 122	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9KF57 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 005FC0855D20D84D	FASTA	743	80,008
	10 20 30 40 50 60 MSLHREPTAT EVKEQKIYRE MGLTDEEFAL VESILGRLPN YTETGLFSVM WSEHCSYKNS 70 80 90 100 110 120 KVLLKKFPID GDKVLQGPGE GAGIIDIGDE QAVVFKIESH NHPSAIEPYQ GAATGVGGIL 130 140 150 160 170 180 RDVFSMGARP ISLLNSLRFG ELTSPKVRYL FEEVVAGIAG YGNCVGVPTV GGEVQFDPCY 190 200 210 220 230 240 EGNPLVNAMC VGLIDHKDIQ KGQAKGVGNT VMYVGASTGR DGIHGATFAS EELSEASEEK 250 260 270 280 290 300 RPAVQVGDPF MEKLLLEACL ELIKSDALVG IQDMGAAGLT SSSAEMASKA GSGIEMNLDH 310 320 330 340 350 360 VPQREKGMTP YEMMLSESQE RMLIVVKKGR EQEIKDIVAH WGLHAVEVGQ VTDDKKLRLL 370 380 390 400 410 420 HKGEVVADVP VDALAEDAPV YHKPSKVPAY YEAFQSEKTQ WIPTIANVKD TLLSLLQQPT 430 440 450 460 470 480 IASKEWVYEQ YDYMVQTNTV VSPGSDAAVV RIRGTKKALA MTTDCNSRYL FLDPEVGGKI 490 500 510 520 530 540 AIAEAARNIV CSGGVPLGVT DCLNYGNPEK PEIFWQLEKS TDGMSEACRE LGTPVIGGNV 550 560 570 580 590 600 SLYNETNGVA VYPTPVIGMV GLIEDVGHIT TQAFKKAGDL IYVIGEAKAE FGGSELQKLV 610 620 630 640 650 660 NGEISGKAPA IDLAVEKKRQ EQLLEAIRAG AVASAHDIAE GGLAVALAES MMGEAVGADV 670 680 690 700 710 720 VIDGEWTTEL FAESQSRFLV SVPKEKQAIF ESLVEDAIHL GQVTDHPQLN IQDVNGAQVL 730 740 QASVNEMLEA WKGAIPCLLK SKA

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04348.1.
PIR	E83728.
RefSeq	NP_241495.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KF57.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000053615; EBBACP00000052254; EBBACG00000053606.
GeneID	893127.
GenomeReviews	Gene locus BH0629 in contig BA000004_GR.
KEGG	bha:BH0629.
NMPDR	fig|272558.1.peg.629.
PATRIC	18938248. VBIBacHal18977_0662.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002932.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q9KF57.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0629-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_BACHD

Accession

Primary (citable) accession number: Q9KF57

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program