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Q9KF56 (Q9KF56_BACHD) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Name:purF EMBL BAB04349.1
Ordered Locus Names:BH0630
OrganismBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]
Taxonomic identifier272558 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site121For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2461Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding3921Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4471Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4501Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
Q9KF56 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 9CC9A1D49B3BD6F1

FASTA47351,245
        10         20         30         40         50         60 
MFAEIKGLNE ECGVFAIWGH EDAAQVAYYG LHSLQHRGQE GAGIVVTDGH QLSVHKGLGL 

        70         80         90        100        110        120 
VNDVFNPDIF DSLKGKAAVG HVRYATAGGG GYANVQPLVF RSQIGSLAIA HNGNLVNANH 

       130        140        150        160        170        180 
LKNQLENQGS IFQSTSDTEV LAHLIKRSGY HHIREQLKSS LSMLKGAYAF AVMTERELMV 

       190        200        210        220        230        240 
ALDPNGLRPL SIGRLGEAYV VASETCAFDV IGAEYVREVE PGELVTINDD GLTSERFVGE 

       250        260        270        280        290        300 
AARAICSMEY VYFARPDSNV DTINVHTARK NLGKQLAIEA PVEADVVTGV PDSSISAAIG 

       310        320        330        340        350        360 
YAEQSGIPYE LGLIKNRYVG RTFIQPSQAL REQGVKMKLS AVRGVVEGKR VVMIDDSIVR 

       370        380        390        400        410        420 
GTTSRRIVNM LREAGAKEVH VRISAPPIKH PCFYGIDTST KEELIAATHS IEEMRKQMGA 

       430        440        450        460        470 
DSLSFLSLEG LVKGVGRSNE VSSCGQCLAC FTGQYPTEIY PDTLHPHDKL LPV

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References

[1]"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000004 Genomic DNA. Translation: BAB04349.1.
PIRF83728.
RefSeqNP_241496.1. NC_002570.2.

3D structure databases

HSSPHSSP built from PDB template 1AO0 based on UniProtKB P00497.
ProteinModelPortalQ9KF56.
SMRQ9KF56. Positions 12-469.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000053687; EBBACP00000052326; EBBACG00000053678.
GeneID893134.
GenomeReviewsGene locus BH0630 in contig BA000004_GR.
KEGGbha:BH0630.
NMPDRfig|272558.1.peg.630.
PATRIC18938250. VBIBacHal18977_0663.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000000004.
HOGENOMHBG392416.
OMAVWAPGEE.
PhylomeDBQ9KF56.
ProtClustDBPRK07631.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR000583. GATase_2.
IPR017932. GATase_II.
IPR000836. PRibTrfase.
[Graphical view]
KOK00764.
PANTHERPTHR11907. Amd_phspho_trans. 1 hit.
PfamPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. PurF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ9KF56_BACHD
AccessionPrimary (citable) accession number: Q9KF56
Entry history
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info