Reviewed, UniProtKB/Swiss-Prot Q9KF55 (PUR5_BACHD)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
Alternative name(s):
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    AIR synthase

Gene names

Name:	purM
Ordered Locus Names:	BH0631

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741
Subcellular location	Cytoplasm By similarity HAMAP MF_00741.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741		PRO_0000148196

Sequences

	Sequence		Length	Mass (Da)
	Q9KF55-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 32738F0F36726101	FASTA	345	36,489
	10 20 30 40 50 60 MSDAYKQAGV DIEAGYEAVA RMKKHVLRTA RPEVIGGLGS FGGMFDLSAL NMKEPVLVSG 70 80 90 100 110 120 TDGVGTKLML AFQMNKHDTI GVDAVAMCVN DIVVQGAEPL YFLDYIACGK SEPARIEAIV 130 140 150 160 170 180 KGIADGCEQA GCALIGGETA EMPGMYDETE YDVAGFAVGA VEKADLVTGA DIESGDLILG 190 200 210 220 230 240 LPSSGVHSNG FSLVRKILLH DHGYTLDSEI PALGKKLGEE LLTPTKIYVK TVLQTLKTGK 250 260 270 280 290 300 VKGLSHITGG GFYENVPRML PEGLGAEIDY GSWPVPPIFS FIRETGQLSA KDMFSTFNMG 310 320 330 340 IGMAVVVSSD DAPHVIRQIQ ETGEDAYIIG RVTDTSGVVI GGAFE

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04350.1.
PIR	G83728.
RefSeq	NP_241497.1.
3D structure databases
SMR	Q9KF55. Positions 13-338.
ModBase	Search...
Genome annotation databases
GeneID	893125.
GenomeReviews	Gene locus BH0631 in contig BA000004_GR.
KEGG	bha:BH0631.
NMPDR	fig|272558.1.peg.631.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG531222.
OMA	VHGLAHI.
PhylomeDB	Q9KF55.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0631-MONOMER.
BRENDA	6.3.3.1. 191865.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_BACHD

Accession

Primary (citable) accession number: Q9KF55

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)