Reviewed, UniProtKB/Swiss-Prot Q9KF53 (PUR9_BACHD)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	BH0633

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_0000192069

Sequences

	Sequence		Length	Mass (Da)
	Q9KF53-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 13EF1768AA84B485	FASTA	511	55,578
	10 20 30 40 50 60 MKRRALVSVS NKEGIVPFAK ALVEHEVEIV STGGTKRALQ EAGIPVTGIS DVTGFPEILD 70 80 90 100 110 120 GRVKTLHPNI HGGLLAMRER DEHLAQLNEH HIRPIDFVVV NLYPFQQTIA KPEATFADAI 130 140 150 160 170 180 ENIDIGGPSM LRAAAKNHQH VTVVVDPVDY ETVLKELADQ GNVATETKRR LAAKVFRHTA 190 200 210 220 230 240 AYDAMIAEYL TDAVGEESPE SLTVTFEKKQ DLRYGENPHQ KATFYQKPLG AKASIAHAKQ 250 260 270 280 290 300 LHGKELSYNN INDADAALSI VKEFKEPAAV AVKHMNPCGV GTGETIKEAF DKAYEADPVS 310 320 330 340 350 360 IFGGIIALNR EVDVETAKTL KEIFLEIIIA PSFSEEALDV LTSKKNLRLL TLPLNEENQA 370 380 390 400 410 420 EKRITSIHGG ALVQEEDTYG FEEAEIKIPT KREPTEAEWE ALKLAWRVVK HVKSNAIVLA 430 440 450 460 470 480 DGQMTVGVGA GQMNRVGAAK IAIEQAGEKA AGSVMGSDAF FPMGDTVELA AKAGITAIIQ 490 500 510 PGGSIRDEES IENADKHGIA MVFTGVRHFK H

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04352.1.
PIR	A83729.
RefSeq	NP_241499.1.
3D structure databases
SMR	Q9KF53. Positions 3-511.
ModBase	Search...
Genome annotation databases
GeneID	893121.
GenomeReviews	Gene locus BH0633 in contig BA000004_GR.
KEGG	bha:BH0633.
NMPDR	fig|272558.1.peg.633.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG498048.
OMA	VVKHVKS.
PhylomeDB	Q9KF53.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0633-MONOMER.
BRENDA	2.1.2.3. 191865. 3.5.4.10. 191865.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_BACHD

Accession

Primary (citable) accession number: Q9KF53

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)