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Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi283Magnesium or manganeseUniRule annotation1
Metal bindingi285Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi133 – 194ATPUniRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBHAL272558:G1G3A-706-MONOMER
UniPathwayiUPA00074; UER00125

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:BH0634
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514351 – 428Phosphoribosylamine--glycine ligaseAdd BLAST428

Proteomic databases

PRIDEiQ9KF52

Interactioni

Protein-protein interaction databases

STRINGi272558.BH0634

Structurei

3D structure databases

ProteinModelPortaliQ9KF52
SMRiQ9KF52
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini107 – 313ATP-graspUniRule annotationAdd BLAST207

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C12 Bacteria
COG0151 LUCA
KOiK01945
OMAiKATVCKY
OrthoDBiPOG091H02DZ

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.90.600.10, 1 hit
HAMAPiMF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9KF52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLVIGSGG REHTIAWKFA QSEKVERVYV APGNDGMSDV ATCVAISEQD
60 70 80 90 100
HDQLVAFAKE NKIGLTFVGP EVPLLAGIVD RFQEEGLRVF GPSKRAAEIE
110 120 130 140 150
GSKSYAKQVM KTYNIPTGSY EVFTSFDEAK AYVEAEGVPI VIKADGLAAG
160 170 180 190 200
KGVVVALTNE EAIAALDDML NQDKFGGAGA RVVIEEYLEG EELSLMAFVH
210 220 230 240 250
GETVIPMVGA QDHKRAFDGD QGPNTGGMGA YSPVPQFSDV QLKQAVNEIL
260 270 280 290 300
IPTARALMQE ERSFTGILYA GLMMTADGPK VIEFNARFGD PETQVVLPRL
310 320 330 340 350
KSDLVNVIES LLDGQEPELE WDEQAVLGVV LATKGYPGSY EKGYTISGLE
360 370 380 390 400
QLEDDTLVFH AGTKREEEEL VTNGGRVLLV AKQASTLREA QAAVYEELNK
410 420
VKSDGLFYRK DIGSKAIAER AVSSQTEQ
Length:428
Mass (Da):46,304
Last modified:October 1, 2000 - v1
Checksum:i85B59E32FAC8D168
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA Translation: BAB04353.1
PIRiB83729
RefSeqiWP_010896810.1, NC_002570.2

Genome annotation databases

EnsemblBacteriaiBAB04353; BAB04353; BAB04353
KEGGibha:BH0634

Similar proteinsi

Entry informationi

Entry nameiPUR2_BACHD
AccessioniPrimary (citable) accession number: Q9KF52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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