Reviewed, UniProtKB/Swiss-Prot Q9KF52 (PUR2_BACHD)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	BH0634

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_00138
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151435
Regions
	Domain	107 – 313	207	ATP-grasp
	Nucleotide binding	133 – 194	62	ATP By similarity
Sites
	Metal binding	283	1	Magnesium or manganese By similarity
	Metal binding	285	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KF52-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 85B59E32FAC8D168	FASTA	428	46,304
	10 20 30 40 50 60 MNVLVIGSGG REHTIAWKFA QSEKVERVYV APGNDGMSDV ATCVAISEQD HDQLVAFAKE 70 80 90 100 110 120 NKIGLTFVGP EVPLLAGIVD RFQEEGLRVF GPSKRAAEIE GSKSYAKQVM KTYNIPTGSY 130 140 150 160 170 180 EVFTSFDEAK AYVEAEGVPI VIKADGLAAG KGVVVALTNE EAIAALDDML NQDKFGGAGA 190 200 210 220 230 240 RVVIEEYLEG EELSLMAFVH GETVIPMVGA QDHKRAFDGD QGPNTGGMGA YSPVPQFSDV 250 260 270 280 290 300 QLKQAVNEIL IPTARALMQE ERSFTGILYA GLMMTADGPK VIEFNARFGD PETQVVLPRL 310 320 330 340 350 360 KSDLVNVIES LLDGQEPELE WDEQAVLGVV LATKGYPGSY EKGYTISGLE QLEDDTLVFH 370 380 390 400 410 420 AGTKREEEEL VTNGGRVLLV AKQASTLREA QAAVYEELNK VKSDGLFYRK DIGSKAIAER AVSSQTEQ

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04353.1.
PIR	B83729.
RefSeq	NP_241500.1.
3D structure databases
SMR	Q9KF52. Positions 1-417.
ModBase	Search...
Genome annotation databases
GeneID	893119.
GenomeReviews	Gene locus BH0634 in contig BA000004_GR.
KEGG	bha:BH0634.
NMPDR	fig|272558.1.peg.634.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404060.
OMA	FSLMAFV.
PhylomeDB	Q9KF52.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0634-MONOMER.
BRENDA	6.3.4.13. 191865.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_BACHD

Accession

Primary (citable) accession number: Q9KF52

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)