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Q9KF52 (PUR2_BACHD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:BH0634
OrganismBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]
Taxonomic identifier272558 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151435

Regions

Domain107 – 313207ATP-grasp
Nucleotide binding133 – 19462ATP By similarity

Sites

Metal binding2831Magnesium or manganese By similarity
Metal binding2851Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KF52 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 85B59E32FAC8D168

FASTA42846,304
        10         20         30         40         50         60 
MNVLVIGSGG REHTIAWKFA QSEKVERVYV APGNDGMSDV ATCVAISEQD HDQLVAFAKE 

        70         80         90        100        110        120 
NKIGLTFVGP EVPLLAGIVD RFQEEGLRVF GPSKRAAEIE GSKSYAKQVM KTYNIPTGSY 

       130        140        150        160        170        180 
EVFTSFDEAK AYVEAEGVPI VIKADGLAAG KGVVVALTNE EAIAALDDML NQDKFGGAGA 

       190        200        210        220        230        240 
RVVIEEYLEG EELSLMAFVH GETVIPMVGA QDHKRAFDGD QGPNTGGMGA YSPVPQFSDV 

       250        260        270        280        290        300 
QLKQAVNEIL IPTARALMQE ERSFTGILYA GLMMTADGPK VIEFNARFGD PETQVVLPRL 

       310        320        330        340        350        360 
KSDLVNVIES LLDGQEPELE WDEQAVLGVV LATKGYPGSY EKGYTISGLE QLEDDTLVFH 

       370        380        390        400        410        420 
AGTKREEEEL VTNGGRVLLV AKQASTLREA QAAVYEELNK VKSDGLFYRK DIGSKAIAER 


AVSSQTEQ 

« Hide

References

[1]"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000004 Genomic DNA. Translation: BAB04353.1.
PIRB83729.
RefSeqNP_241500.1. NC_002570.2.

3D structure databases

ProteinModelPortalQ9KF52.
SMRQ9KF52. Positions 1-417.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272558.BH0634.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB04353; BAB04353; BAB04353.
GeneID893119.
KEGGbha:BH0634.
PATRIC18938258. VBIBacHal18977_0667.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
KOK01945.
OMAYKGFLYA.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

BioCycBHAL272558:GJC5-692-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_BACHD
AccessionPrimary (citable) accession number: Q9KF52
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways