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Q9KF37 (DNLJ_BACHD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD(+)]
Gene names
Name:ligA
Synonyms:lig
Ordered Locus Names:BH0649
OrganismBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]
Taxonomic identifier272558 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length669 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP-Rule MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_01588

Cofactor

Magnesium or manganese By similarity.

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA ligase (NAD+) activity

Inferred from electronic annotation. Source: HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 669669DNA ligase HAMAP-Rule MF_01588
PRO_0000161736

Regions

Domain591 – 66979BRCT
Nucleotide binding34 – 385NAD By similarity
Nucleotide binding83 – 842NAD By similarity

Sites

Active site1151N6-AMP-lysine intermediate By similarity
Metal binding4041Zinc By similarity
Metal binding4071Zinc By similarity
Metal binding4221Zinc By similarity
Metal binding4271Zinc By similarity
Binding site1131NAD By similarity
Binding site1361NAD By similarity
Binding site1701NAD By similarity
Binding site2861NAD By similarity
Binding site3101NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9KF37 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: FF9784A53EE551F0

FASTA66974,242
        10         20         30         40         50         60 
MERNDAERKI EKLRNQLEEY GYHYYVLDKP LVSDAEYDGL MNELIELEEA FPELKSDTSP 

        70         80         90        100        110        120 
SVRVGGPPLP HFEKVEHRTP MLSLGNAFSD QDLRDFDRRV RQVVGDEVTY SCELKIDGLA 

       130        140        150        160        170        180 
ISLIYESGRF VRGATRGDGT TGEDITQNLR TIPSIPLRLK EAVSLEVRGE AFMPKRSFEA 

       190        200        210        220        230        240 
LNEAKEAAGE ERFANPRNAA AGSLRQLDPK LAAKRHLDAF IYAIGSVEGK ELHSHHEGLN 

       250        260        270        280        290        300 
YLKTIGFKIN PESAYCESID EVIDYVNSWL ERRADLPYEI DGIVIKVDNV NFQEQLGYTA 

       310        320        330        340        350        360 
KSPRWAIAYK FPAEEVITTL LDITLNVGRT GVVTPTAELK PVTVAGTTVK RASLHNEDLI 

       370        380        390        400        410        420 
REKDIRLGDS VVVKKAGDII PEVVNVLTEL RTGEERPFSM PTHCPECGSE LVRLEGEVAL 

       430        440        450        460        470        480 
RCINPQCPAQ IREGLIHFVS RQAMNIDGLG EKVITQLFEH GLIHNVADLY KLNKDELLQL 

       490        500        510        520        530        540 
ERMGEKSVNN LLASIETSKK SSLERLLFGL GIRFVGSKAA KTLAMHFPTM TELRRASYDE 

       550        560        570        580        590        600 
LIAVNEIGEK MAASIVSYFE KPEVNELIDE LASLGVNMTY NGPKPIGEEE IADSPFAGKT 

       610        620        630        640        650        660 
VVLTGKLQAL TRGEAKEKIE ALGGKVTGSV SKNTDLLVAG EDAGSKLTKA KELNIEIWDE 


EALVKAISH

« Hide

References

[1]"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000004 Genomic DNA. Translation: BAB04368.1.
PIRA83731.
RefSeqNP_241515.1. NC_002570.2.

3D structure databases

ProteinModelPortalQ9KF37.
SMRQ9KF37. Positions 1-314.
ModBaseSearch...

Protein-protein interaction databases

STRING272558.BH0649.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB04368; BAB04368; BAB04368.
GeneID893092.
KEGGbha:BH0649.
PATRIC18938286. VBIBacHal18977_0681.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0272.
KOK01972.
OMAYITKENF.
ProtClustDBPRK07956.

Enzyme and pathway databases

BioCycBHAL272558:GJC5-707-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
HAMAPMF_01588. DNA_ligase_A.
InterProIPR001357. BRCT_dom.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
PANTHERPTHR11107:SF5. PTHR11107:SF5. 1 hit.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00278. HhH1. 3 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_BACHD
AccessionPrimary (citable) accession number: Q9KF37
Entry history
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: October 1, 2000
Last modified: May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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