Q9KER1 (MNGB_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative mannosylglycerate hydrolase
EC=3.2.1.170

Alternative name(s):
2-O-(6-phospho-mannosyl)-D-glycerate hydrolase
Alpha-mannosidase mngB

Gene names

Name:	mngB
Ordered Locus Names:	BH0788/BH0789

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	896 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May hydrolyze mannosyl-D-glycerate to mannose-6-phosphate and glycerate By similarity.
Catalytic activity	2-O-(6-phospho-alpha-D-mannosyl)-D-glycerate + H2O = D-mannose 6-phosphate + D-glycerate.
Cofactor	Binds one divalent metal cation per subunit By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 38 family.
Sequence caution	The sequence BAB04507.1 differs from that shown. Reason: Frameshift at position 123. Produces two separate ORFs. The sequence BAB04508.1 differs from that shown. Reason: Frameshift at position 123. Produces two separate ORFs.

Ontologies

Keywords
Ligand	Metal-binding
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mannose metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-mannosidase activity Inferred from electronic annotation. Source: InterPro carbohydrate binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 896	896	Putative mannosylglycerate hydrolase		PRO_0000343407
Sites
	Active site	125	1	Nucleophile By similarity
	Metal binding	12	1	Divalent metal cation By similarity
	Metal binding	14	1	Divalent metal cation By similarity
	Metal binding	125	1	Divalent metal cation By similarity
	Metal binding	348	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KER1 [UniParc]. Last modified July 1, 2008. Version 2. Checksum: FF55FA8327A0972B	FASTA	896	102,540
	10 20 30 40 50 60 MKKTAHIISH SHWDREWYMP FEGHRYYLIQ LMDDLLELFA TDPNFRSFHM DGQTIMLEDY 70 80 90 100 110 120 LNIRPEKEAE VRKYIQDGRL VIGPWYILQD AFLTSAEANV RNLLYGIKDT ETFGQKREQI 130 140 150 160 170 180 GYFPDTFGIY GQAPQLLAQA GIRAAVFGRG VTPTGFNNQV QHDDYSSPFS ELIWEAPDGS 190 200 210 220 230 240 QVIGILLANW YSNGNEIPTD EDEAQTFWVK KLRDAERFAS TSQLLFMNGC DHQPVQKDVT 250 260 270 280 290 300 QAIKVAETLF PDVAFKHSNF HDYLTQIKEE LPKELQKITG ELRNQKTDGW STLVNTASAR 310 320 330 340 350 360 IYLKQANDRC QTLLTNVLEP MCLLVENKSL HRDFSEYYWK LLMENHPHDS ICGCSIDAVH 370 380 390 400 410 420 REMKTRFEKV EAGATTFIAE QGKEIAAQIN TLHDSEEAIP LVVLKTNGTS GKRVVRHKVA 430 440 450 460 470 480 MKKIYFDEMD FRHIPDRLKE IVMPTYRLEF PNKGSVPIEV QDAGVRFGYD LPRDGFRRPY 490 500 510 520 530 540 YARELEVTFS YDSDLYLGYE CGFLVPVEEK QTEARKELIG DPSMNTLENE AMKVMIHRNG 550 560 570 580 590 600 SYSILDKTTG FEYRHLGIYE DVGDIGNEYM FKASSDGVRY TTEACEASIR IIENNSLCAT 610 620 630 640 650 660 VEICQTLSVP AAADERLKEE QERLVWHPDR KAGRSKERTD ITLRTELTLE QGAKGLKVNV 670 680 690 700 710 720 NIDNTAKDHR MRALFPVERA RGNHYADSIY EIVERPNTPD PKWQNPAFDH HMQRLVSLDN 730 740 750 760 770 780 GEYGLTIATK GLHEYEIVSD SIAVTLLRSV GELGDWGLFE TPEAQCFGQN EAQFVLLPHK 790 800 810 820 830 840 GDVLSANVYV AAYDDPVEPT VIQTEQSMGP LPHATNLFQW SGEGLVLTAC KPTMDGRGMI 850 860 870 880 890 LRWFNPKREG EALIVQSTHF LQIYRSTILE EQIEKLGTEN VQIEVRPQEI VTLRFE

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04507.1. Frameshift. BA000004 Genomic DNA. Translation: BAB04508.1. Frameshift.
PIR	D83748. E83748.
RefSeq	NP_241654.1. NC_002570.2. NP_241655.1. NC_002570.2.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH38. Glycoside Hydrolase Family 38.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000052734; EBBACP00000051373; EBBACG00000052725.
GeneID	892817. 892821.
NMPDR	fig|272558.1.peg.788.
PATRIC	18938576. VBIBacHal18977_0826.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000006292.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0788-MONOMER.
Family and domain databases
InterPro	IPR011013. Glyco_hydro-type_carb-bd. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR011682. Glyco_hydro_38_C. IPR015341. Glyco_hydro_38_cen_dom. IPR000602. Glyco_hydro_38_core. [Graphical view]
Gene3D	G3DSA:3.20.110.10. Glyco_hydro_38_core. 1 hit.
Pfam	PF09261. Alpha-mann_mid. 1 hit. PF01074. Glyco_hydro_38. 1 hit. PF07748. Glyco_hydro_38C. 1 hit. [Graphical view]
SMART	SM00872. Alpha-mann_mid. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNGB_BACHD

Accession

Primary (citable) accession number: Q9KER1
Secondary accession number(s): Q9KER0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program