Reviewed, UniProtKB/Swiss-Prot Q9KEL8 (SYR2_BACHD)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA synthetase 2
    EC=6.1.1.19
Alternative name(s):
    Arginine--tRNA ligase 2
      Short name=ArgRS 2

Gene names

Name:	argS2
Ordered Locus Names:	BH0834

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity. HAMAP MF_00123
Subcellular location	Cytoplasm HAMAP MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Arginyl-tRNA synthetase 2 HAMAP MF_00123		PRO_0000151529
Regions
	Motif	117 – 127	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	Q9KEL8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C9C544BF1DE1A1FF	FASTA	561	64,133
	10 20 30 40 50 60 MLAQMISEEL ARILSLKEDE VERLLEIPPQ ETLGDLAFPC FTLAKTKRKA PPFIAAELEA 70 80 90 100 110 120 AFLEHKEVTA KATGGYVNFF FHRETVAGQL FQEMKSNQYW QPNSGDGKRV VIDMSSPNIA 130 140 150 160 170 180 KPFGIGHLRS TIIGHALYHL LKKTGYDPIR VNHLGDWGTQ FGKQIAAYQR WGGDVDLKQN 190 200 210 220 230 240 PIASFLELYV RFHEEAEKDE SLEDEGREWF KKLEEGDEEA DRLWTYFVKE SLNEFDRMYN 250 260 270 280 290 300 RLGVEFDYVL GESFYNDQMA PVVKELQEKG LLTCSEGALV VPLEDADLPP CLIVKSDGTS 310 320 330 340 350 360 IYATRDLATA IYRHHVQKGE KLLYVVGGEQ TLHFKQVFHV LKKMGYKWAD QCEHITFGLL 370 380 390 400 410 420 RLDGKKMSTR RGRVVMLEDV LNDVVAHAKA KIKEKHPNHP HLHEVAEAVG VGAVIFGDLK 430 440 450 460 470 480 QDRRLDVNFR LEDALSFEGE TGPYVQYTYA RIQSILRKGD KLNDSRHMDW QHVTKDEGWS 490 500 510 520 530 540 LLKVLIQYPN VLIKATEARE PHQLARYVLT VSKKFNQFYH KYVILADDET VRQARLILAE 550 560 RTGEVIADAM AVLGVKTPEE L

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04553.1.
PIR	B83754.
RefSeq	NP_241700.1.
3D structure databases
SMR	Q9KEL8. Positions 2-561.
ModBase	Search...
Genome annotation databases
GeneID	892340.
GenomeReviews	Gene locus BH0834 in contig BA000004_GR.
KEGG	bha:BH0834.
NMPDR	fig|272558.1.peg.834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695395.
OMA	AYKKWGN.
PhylomeDB	Q9KEL8.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0834-MONOMER.
BRENDA	6.1.1.19. 191865.
Family and domain databases
HAMAP	MF_00123. Arg_tRNA_synth. [Tree]
InterPro	IPR001278. Arg-tRNA-synth_Ic. IPR015945. Arg-tRNA-synth_Ic_core. IPR005148. Arg-tRNA-synth_Ic_N. IPR008909. DALR_anticod_bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM00836. DALR_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR2_BACHD

Accession

Primary (citable) accession number: Q9KEL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)