Ribonuclease H - Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

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Q9KEI9 (RNH1_BACHD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ribonuclease H
Short name=RNase H
EC=3.1.26.4

Gene names

Name:	rnhA
Ordered Locus Names:	BH0863

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Ref.2
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester.
Cofactor	Binds 2 metal ions per subunit. Manganese or magnesium.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNase H family. Contains 1 RNase H domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 196

196

Ribonuclease H

PRO_0000195430

Regions

Domain

88 – 196

109

RNase H

Sites

Metal binding

Magnesium 1

Metal binding

Magnesium 2

Metal binding

109

Magnesium 2

Metal binding

132

Magnesium 2

Metal binding

192

Magnesium 1

Experimental info

Mutagenesis

109

E → Q: Loss of activity. Ref.2

Mutagenesis

132

D → N: Loss of activity. Ref.2

Mutagenesis

188

E → A: Strongly reduces activity. Ref.2

Mutagenesis

188

E → Q: No effect. Ref.2

Mutagenesis

192

D → N: Strongly reduced activity with manganese. Loss of activity with magnesium. Ref.2

Secondary structure

196

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9KEI9 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 4130558FA37D2A86
Show »

FASTA

196

22,373

        10         20         30         40         50         60 
MAKSKYYVVW NGRKPGIYTS WSACEAQVKG YTGAKFKSYP SKEEAEAAFR GEEATPKLAK 

        70         80         90        100        110        120 
EEIIWESLSV DVGSQGNPGI VEYKGVDTKT GEVLFEREPI PIGTNNMGEF LAIVHGLRYL 

       130        140        150        160        170        180 
KERNSRKPIY SDSQTAIKWV KDKKAKSTLV RNEETALIWK LVDEAEEWLN THTYETPILK 

       190 
WQTDKWGEIK ADYGRK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
[2]	"Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis." Nowotny M., Gaidamakov S.A., Crouch R.J., Yang W. Cell 121:1005-1016(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 59-196 OF MUTANT ASN-132 IN COMPLEX WITH MAGNESIUM AND SUBSTRATE, FUNCTION, MUTAGENESIS OF GLU-109; ASP-132; GLU-188 AND ASP-192.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000004 Genomic DNA. Translation: BAB04582.1.

PIR

G83757.

RefSeq

NP_241729.1. NC_002570.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZBF	X-ray	1.50	A	59-196	[»]
1ZBI	X-ray	1.85	A/B	59-196	[»]
1ZBL	X-ray	2.20	A/B	59-191	[»]
2G8F	X-ray	1.65	A	59-196	[»]
2G8H	X-ray	1.85	A	59-196	[»]
2G8I	X-ray	1.65	A	59-196	[»]
2G8K	X-ray	1.65	A	59-196	[»]
2G8U	X-ray	2.70	A	59-196	[»]
2G8V	X-ray	1.85	A	59-196	[»]
2G8W	X-ray	2.05	A	59-196	[»]
2R7Y	X-ray	1.80	A	62-193	[»]
3D0P	X-ray	1.80	A/C	61-194	[»]
3EY1	X-ray	1.60	A	59-196	[»]
3I8D	X-ray	1.61	A/C	62-193	[»]
3TWH	X-ray	1.79	A	59-196	[»]
3ULD	X-ray	1.60	A	59-196	[»]
4HTU	X-ray	1.49	A/B	61-194	[»]
4HUE	X-ray	1.56	A/B	61-194	[»]
4HUF	X-ray	1.69	A/B	61-194	[»]
4HUG	X-ray	1.64	A/B	61-194	[»]

ProteinModelPortal

Q9KEI9.

SMR

Q9KEI9. Positions 61-196.

ModBase

Search...

Protein-protein interaction databases

STRING

272558.BH0863.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAB04582; BAB04582; BAB04582.

GeneID

893801.

KEGG

bha:BH0863.

PATRIC

18938738. VBIBacHal18977_0907.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG3341.

HOGENOM

HOG000251719.

K03469.

OMA

PADFGRK.

ProtClustDB

CLSK792960.

Enzyme and pathway databases

BioCyc

BHAL272558:GJC5-921-MONOMER.

Family and domain databases

Gene3D

3.40.970.10. 1 hit.

InterPro

IPR009027. Ribosomal_L9/RNase_H1_N.
IPR017290. RNase_H1_bac.
IPR011320. RNase_H1_N.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]

Pfam

PF01693. Cauli_VI. 1 hit.
PF00075. RNase_H. 1 hit.
[Graphical view]

PIRSF

PIRSF037839. Ribonuclease_H. 1 hit.

SUPFAM

SSF55658. L9_N_like. 1 hit.
SSF53098. RNaseH_fold. 1 hit.

PROSITE

PS50879. RNASE_H. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9KEI9.

Entry information

Entry name

RNH1_BACHD

Accession

Primary (citable) accession number: Q9KEI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents