Q9KEE6 (KATG_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	BH0906

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Catalase-peroxidase HAMAP MF_01961		PRO_0000354724
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	263	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 222		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-248) By similarity
	Cross-link	222 ↔ 248		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KEE6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E93B4B5EBFA3A593	FASTA	735	81,531
	10 20 30 40 50 60 MDTNNTGKCP FHHGATTSPK SSGTTNNDWW PNALNLNILR QHDKKSNPMG EEFDYAEEFS 70 80 90 100 110 120 KLDYDALKQD VRDLMRDSQD WWPADFGHYG PFFIRMSWHA AGTYRIGDGR GGGGTGNQRF 130 140 150 160 170 180 APLNSWPDNG NLDKARRLLW PIKQKYGNKI SWADLLVLAG NVAIEDMGGP VIGFGAGRED 190 200 210 220 230 240 IWHPEEDIYW GSEKEWLTGD KRYSGDRELE NPLAAVEMGL IYVNPEGPDG KPDPIKAAHD 250 260 270 280 290 300 IRETFGRMGM NDEETVALIA GGHTFGKAHG AGNPDHVGPE PEAAPIEAQG LGWQNTYGSG 310 320 330 340 350 360 KGRDTITSGL EGAWTANPTQ WDNGFFDLLF GYEWWLTKSP AGAYQWQAVD PDEKDLAPDA 370 380 390 400 410 420 EDPSVKVPTV MLTTDLALRH DPEYEKISRR FHKNPDEFAD AFARAWFKLL HRDMGPKARY 430 440 450 460 470 480 LGPEVPAEDF IWQDPVPTVD YELTDAEVEE LKAKILDSGL TVSELVTTAW ASASTFRNSD 490 500 510 520 530 540 KRGGANGARI RLAPQKDWEV NQPEQLEKVL SVLENIQSQL DKKVSIADLI VLGGSAAVEK 550 560 570 580 590 600 AAKEAGFDVT VPFAPGRGDA TQEQTDVEGF AVLEPVSDGF RNYQKKEYSV SPEELLIDKA 610 620 630 640 650 660 QLLDLTAPEM TALIGGMRAL GANYGGTQHG VFTDCVGTLT NDFFVNLLDM GIEWKPVDYN 670 680 690 700 710 720 LYEGRDRKTG EVVRTATRVD LVFGSNSILR AIAEVYAQDD NKGKFVEDFI AAWVKVMNAD 730 RFDLKLNKKA QLTSK

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04625.1.
PIR	B83763.
RefSeq	NP_241772.1. NC_002570.2.
3D structure databases
HSSP	HSSP built from PDB template 1U2L based on UniProtKB P13029.
ProteinModelPortal	Q9KEE6.
SMR	Q9KEE6. Positions 22-724.
ModBase	Search...
Protein family/group databases
PeroxiBase	2372. BhaCP01_C-125.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000050273; EBBACP00000048912; EBBACG00000050264.
GeneID	891763.
GenomeReviews	Gene locus BH0906 in contig BA000004_GR.
KEGG	bha:BH0906.
NMPDR	fig|272558.1.peg.906.
PATRIC	18938828. VBIBacHal18977_0952.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000036810.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	Q9KEE6.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0906-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_BACHD

Accession

Primary (citable) accession number: Q9KEE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program