Q9KDW8 (GLPK_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	BH1093

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Glycerol kinase HAMAP MF_00186		PRO_0000059433
Regions
	Nucleotide binding	411 – 415	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	267	1	ATP By similarity
	Binding site	310	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KDW8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 01A822C997243CE1	FASTA	497	54,856
	10 20 30 40 50 60 MTKKYILALD QGTTSSRAIL FNEAGEIIGI EQKEFQQIFP KPGWVEHDAN EIWASVLSVI 70 80 90 100 110 120 AGVLLKTNVE AKEIAAIGIT NQRETAVVWE KESGRPIYNA LVWQSRQTAG ICERLRAEGF 130 140 150 160 170 180 SEMVTEKTGL LIDPYFSGTK VRWILDHVDG AQERAERGEL LFGTIDTWLI WKLSGGKAHV 190 200 210 220 230 240 TDYSNASRTL LYNIYEQCWD DELLKMLNVP RAMLPDVRPS SEVYAETVSY HFFGEEIPIA 250 260 270 280 290 300 GAAGDQQAAL FGQACFEKGM AKNTYGTGCF MLMNTGNQGV KSKHGLLTTI AWGLDGKVEY 310 320 330 340 350 360 ALEGSIFVAG SAIQWLRDGL RMMKSAKESE GYATKVTSAD GVYVVPAFVG LGTPYWDSDV 370 380 390 400 410 420 RGAVFGLTRG TSKEHFIRAT LESLAYQTKD VLQAMEADSG ISLKTLRVDG GAVANNFLMQ 430 440 450 460 470 480 FQSDLLGVSV ERPTVQETTA LGAAYLAGLA VGFWTSKEEI TNNWNLEQKF SAEMEETDRA 490 KLYEGWQKAV RAAQAFK

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04812.1.
PIR	E83786.
RefSeq	NP_241959.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KDW8.
SMR	Q9KDW8. Positions 3-497.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000050050; EBBACP00000048689; EBBACG00000050041.
GeneID	892059.
GenomeReviews	Gene locus BH1093 in contig BA000004_GR.
KEGG	bha:BH1093.
NMPDR	fig|272558.1.peg.1093.
PATRIC	18939246. VBIBacHal18977_1141.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001374.
HOGENOM	HBG511469.
OMA	ALYGQLC.
PhylomeDB	Q9KDW8.
ProtClustDB	PRK00047.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1093-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BACHD

Accession

Primary (citable) accession number: Q9KDW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program