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Protein

Quinolinate synthase A

Gene

nadA

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451IminoaspartateUniRule annotation
Binding sitei62 – 621Iminoaspartate; via amide nitrogenUniRule annotation
Binding sitei140 – 1401IminoaspartateUniRule annotation
Binding sitei161 – 1611IminoaspartateUniRule annotation
Binding sitei255 – 2551IminoaspartateUniRule annotation
Binding sitei272 – 2721IminoaspartateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBHAL272558:GJC5-1298-MONOMER.
UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotation
Ordered Locus Names:BH1220
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Quinolinate synthase APRO_0000155815Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272558.BH1220.

Structurei

3D structure databases

ProteinModelPortaliQ9KDJ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0I. Bacteria.
COG0379. LUCA.
HOGENOMiHOG000222768.
KOiK03517.
OMAiKDEVIQF.
OrthoDBiEOG6DJZ56.

Family and domain databases

HAMAPiMF_00569. NadA_type3.
InterProiIPR003473. NadA.
IPR023515. Quinolinate_synth_A_type3.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9KDJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVFDTLQQS KKLPDVYRQM TRVELEERIR QHKERLGKKL LMLGHHYQRD
60 70 80 90 100
EVFQFADQTG DSLQLAQIAA KEKEAQFIVF CGVHFMAETA DLLTSEEQTV
110 120 130 140 150
LLPDLRAGCS MADMADIHQT ERAWERLQLM FGDTILPLTY VNSTAAIKAF
160 170 180 190 200
CGRNGGATVT SSNAKKMLEW AFTQKERLLF LPDQHLGRNT AYELGIPLEA
210 220 230 240 250
MAVWNPETER LETDQPLENI RVILWKGHCS VHEKFTVKHI EHLRKNEPDM
260 270 280 290 300
SIIVHPECTH DVVIHADDAG STHYIIKTIE SADSGTKWAV GTEMNLVNRL
310 320 330 340 350
ANEHPDKEIV SLNPTMCPCL TMNRIDIEHL CWSLDQLAEG VFQHPIKVED
360
KDRGPALLAL QRMLDRA
Length:367
Mass (Da):41,873
Last modified:October 1, 2000 - v1
Checksum:iD58FC8BE2A54B2D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB04939.1.
PIRiD83802.
RefSeqiWP_010897388.1. NC_002570.2.

Genome annotation databases

EnsemblBacteriaiBAB04939; BAB04939; BAB04939.
KEGGibha:BH1220.
PATRICi18939494. VBIBacHal18977_1265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA. Translation: BAB04939.1.
PIRiD83802.
RefSeqiWP_010897388.1. NC_002570.2.

3D structure databases

ProteinModelPortaliQ9KDJ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272558.BH1220.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB04939; BAB04939; BAB04939.
KEGGibha:BH1220.
PATRICi18939494. VBIBacHal18977_1265.

Phylogenomic databases

eggNOGiENOG4105D0I. Bacteria.
COG0379. LUCA.
HOGENOMiHOG000222768.
KOiK03517.
OMAiKDEVIQF.
OrthoDBiEOG6DJZ56.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.
BioCyciBHAL272558:GJC5-1298-MONOMER.

Family and domain databases

HAMAPiMF_00569. NadA_type3.
InterProiIPR003473. NadA.
IPR023515. Quinolinate_synth_A_type3.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis."
    Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.
    Nucleic Acids Res. 28:4317-4331(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Entry informationi

Entry nameiNADA_BACHD
AccessioniPrimary (citable) accession number: Q9KDJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: October 1, 2000
Last modified: January 20, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.