Reviewed, UniProtKB/Swiss-Prot Q9KDI5 (TGT_BACHD)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	BH1228

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_0000135448
Sites
	Active site	94	1	Nucleophile By similarity
	Metal binding	306	1	Zinc By similarity
	Metal binding	308	1	Zinc By similarity
	Metal binding	311	1	Zinc By similarity
	Metal binding	337	1	Zinc By similarity
	Binding site	95	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9KDI5-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E40D187CE6BAE380	FASTA	379	43,062
	10 20 30 40 50 60 MAAVTYELIK TCKQSGARLG KLHTPHGTIE TPIFMPVGTL ATVKTMSPEE LKQLGAQIIL 70 80 90 100 110 120 SNTYHLWLRP GHDIVKEAGG LHEFMNWDRP ILTDSGGFQV FSLSDLRTIE EEGVHFRNHL 130 140 150 160 170 180 SGEKLFLSPE GAMEIQNALG SDIMMAFDEC PPYPAERDYM RPSVERTSRW AERCLKAHKR 190 200 210 220 230 240 PEDQALFGII QGGEYEDLRR QSAQDITSLD FPGYAIGGVS VGEPKDVMNR VLEFTTPLLP 250 260 270 280 290 300 ANKPRYLMGV GSPDSLIDGA IRGIDMFDCV LPTRIARNGT CMTSNGRLVV RNAKYARDFR 310 320 330 340 350 360 SLDENCDCHV CQTYTRAYIR HLVKCDETFG FRLTTYHNLY FLLKLMKDVR QAILDDRLLD 370 FREEFFEQYG FNQPNAKNF

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000004 Genomic DNA. Translation: BAB04947.1.
PIR	D83803.
RefSeq	NP_242094.1.
3D structure databases
HSSP	HSSP built from PDB template 1K4G based on UniProtKB P28720.
ModBase	Search...
Genome annotation databases
GeneID	894208.
GenomeReviews	Gene locus BH1228 in contig BA000004_GR.
KEGG	bha:BH1228.
NMPDR	fig|272558.1.peg.1228.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9KDI5.
OMA	Q9KDI5. VLNSDIV.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1228-MON.
BRENDA	2.4.2.29. 191865.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. QtRNA_ribo_trans. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_BACHD

Accession

Primary (citable) accession number: Q9KDI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)