Reviewed, UniProtKB/Swiss-Prot Q9KDG1 (SYD_BACHD)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	BH1252

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity. HAMAP MF_00044
Subcellular location	Cytoplasm HAMAP MF_00044.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_0000110825

Sequences

	Sequence		Length	Mass (Da)
	Q9KDG1-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3B08EB2EE928883B	FASTA	595	67,222
	10 20 30 40 50 60 MIGRTHHCGQ LSEEQVNERV QLKGWVQRRR DLGQVIFVDL RDRSGVVQLV FNSDISQEAL 70 80 90 100 110 120 ETAEKVRNEY VLDVEGVVLK RDPSTVNDKI ATGTIEVHVE RLTILNKAKS LPFQIEANTD 130 140 150 160 170 180 ASEDIRLKYR YLDLRRPDMQ ETMKLRHQTT KLIRDFLDGQ EFFEIETPML TKSTPEGARD 190 200 210 220 230 240 YLVPSRVHHG EFYALPQSPQ IFKQLLMVSG FERYYQIVRC FRDEDLRADR QPEFTQIDIE 250 260 270 280 290 300 TSFMDKEDLL TMTENMMAKI MKEVKGLDVA LPFPRMTYDD AMNRYGSDKP DTRFEMELIE 310 320 330 340 350 360 LSDIVKDSDF KVFSSAIKSG GIVKGLNLKG GAGSLSRKEI DGLAEFVKPY GAKGLAWLKV 370 380 390 400 410 420 EEGELKGPIA KFFAGETGAE LQQAMGAEDG DLLFFAADKK EVVFDSLGAL RLKLGKDFNL 430 440 450 460 470 480 IDESKFNFLW VVDFPLVEYD EEAKRFVALH HPFTSPKQED LTKLETDPAS VRADAYDLVL 490 500 510 520 530 540 NGYELGGGSQ RIYQRPVQEK MFAALGFTEE AAQKEFGFLL EAFEYGTPPH GGIALGLDRL 550 560 570 580 590 VMLLAGRLNL RDTIAFPKTA SASCLLTEAP GEVSLEQLLD LNLSIIGHKP DKVNV

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04971.1.
PIR	D83806.
RefSeq	NP_242118.1.
3D structure databases
SMR	Q9KDG1. Positions 4-586.
ModBase	Search...
Genome annotation databases
GeneID	892043.
GenomeReviews	Gene locus BH1252 in contig BA000004_GR.
KEGG	bha:BH1252.
NMPDR	fig|272558.1.peg.1252.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	PLFEYDE.
PhylomeDB	Q9KDG1.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1252-MONOMER.
BRENDA	6.1.1.12. 191865.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BACHD

Accession

Primary (citable) accession number: Q9KDG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)