Q9KDE6 (SYA_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BH1267

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	879 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 879	879	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000075055
Sites
	Metal binding	566	1	Zinc Potential
	Metal binding	570	1	Zinc Potential
	Metal binding	668	1	Zinc Potential
	Metal binding	672	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9KDE6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 4BC9C5CC397A7F41	FASTA	879	97,742
	10 20 30 40 50 60 MKYLTSAQVR QMFLDFFKEK GHDVEPSASL VPHDDPSLLW INSGVATLKK YFDGRVIPEN 70 80 90 100 110 120 PRITNAQKSI RTNDIENVGK TARHHTFFEM LGNFSIGDYF KEEAIEWAWE FLTSEKWIGF 130 140 150 160 170 180 DKEKLSVTVH PEDDEAYSYW KEKIGIPEER IIRLEGNFWD IGEGPSGPNT EIFYDRGPEY 190 200 210 220 230 240 GDQPNDPELY PGGENDRYLE VWNLVFSQFN HNPDGSYTPL PKKNIDTGMG LERMVSVIQN 250 260 270 280 290 300 VPTNFETDLF MPIIRATEKI SGTEYGSHHE ADVSFKVIAD HIRTVTFAIG DGALPSNEGR 310 320 330 340 350 360 GYVLRRLLRR AVRYAKQIGI DRPFMYELVP VVGDIMVDFY PEVKEKAAFI QKVVKTEEER 370 380 390 400 410 420 FHETLNEGLS ILEKVIDKAK SEGASTISGS DVFRLYDTYG FPVDLTEEYV EEQGLQVDLD 430 440 450 460 470 480 GFEAEMERQR ERARTARQQA GSMQVQDEVL GQITVDSTFI GYKQLSTETT IETIVLDKTV 490 500 510 520 530 540 ADYVGAGQEA KVILKETPFY AESGGQVADK GIIRGANGFA VVSDVQKAPN GQHLHTVIVK 550 560 570 580 590 600 EGTLQVNDQV QAIVEETERS GIVKNHTATH LLHRALKDVL GEHVNQAGSL VSEERLRFDF 610 620 630 640 650 660 SHFGQVTDEE KEKIERIVNE KIWQAIKVNI STKTLDEAKA IGAMALFGEK YGDIVRVVEV 670 680 690 700 710 720 GDYSIELCGG CHVTNTSEIG LFKIVSESGI GAGVRRIEAV TGKEAFLFMA KQLDLLKETA 730 740 750 760 770 780 ATVKAKNVKD VPVRVEALQQ QIRELQRENE SLNAKLGNME AGSLVNEVQK IEGVPVLAKA 790 800 810 820 830 840 ISGADMDGLR SIVDKLKQEI PSVVIVLGTA SEGKVNIVAG VTKDLINKGY HAGKLVKEVA 850 860 870 TRCGGGGGGR PDMAQAGGKQ PEKLQDALSF VYEYVKSIS

References

[1]

"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000004 Genomic DNA. Translation: BAB04986.1.
PIR	C83808.
RefSeq	NP_242133.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9KDE6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000053773; EBBACP00000052412; EBBACG00000053764.
GeneID	894263.
GenomeReviews	Gene locus BH1267 in contig BA000004_GR.
KEGG	bha:BH1267.
NMPDR	fig|272558.1.peg.1267.
PATRIC	18939596. VBIBacHal18977_1316.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001776.
HOGENOM	HBG354397.
OMA	MFTNSGM.
PhylomeDB	Q9KDE6.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	BHAL272558:BH1267-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BACHD

Accession

Primary (citable) accession number: Q9KDE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program