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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase 2 (aroA2), 3-phosphoshikimate 1-carboxyvinyltransferase 1 (aroA1)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62ShikimateUniRule annotation1
Active sitei66Proton acceptorUniRule annotation1
Binding sitei78NADPUniRule annotation1
Binding sitei87ShikimateUniRule annotation1
Binding sitei102ShikimateUniRule annotation1
Binding sitei220NADP; via carbonyl oxygenUniRule annotation1
Binding sitei222ShikimateUniRule annotation1
Binding sitei243NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi127 – 131NADPUniRule annotation5
Nucleotide bindingi151 – 156NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciBHAL272558:G1G3A-1407-MONOMER
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Synonyms:aroD
Ordered Locus Names:BH1324
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001258 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001359911 – 278Shikimate dehydrogenase (NADP(+))Add BLAST278

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272558.BH1324

Structurei

3D structure databases

ProteinModelPortaliQ9KD93
SMRiQ9KD93
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 17Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237875
KOiK00014
OMAiFGNPIKH
OrthoDBiPOG091H02DY

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9KD93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLFGLLGH PVGHSLSPLM HNQMFQLLDF DGYYHAFDVE PSQLEKAVEG
60 70 80 90 100
ARALQMQGFN VTIPHKVTIM DYLDMVDEEA QEIGAVNTVV MKDGQLFGSN
110 120 130 140 150
TDGQGYLESL MDVVTTPFPT QDVLIIGAGG AARAIGTVLK REKVRSITIA
160 170 180 190 200
NRTLAKAEEF SGVLESSETS CSALSLKEAE QQLERFSVII NTTSVGMSPR
210 220 230 240 250
VDDMPLSLER LSRETVVSDL IYNPLETKFL REAKQKGAKT VDGLGMFVNQ
260 270
GALAFELWTG LRPDREKMRR CVLEQLGG
Length:278
Mass (Da):30,630
Last modified:October 1, 2000 - v1
Checksum:i2000144C84686264
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000004 Genomic DNA Translation: BAB05043.1
PIRiD83815
RefSeqiWP_010897491.1, NC_002570.2

Genome annotation databases

EnsemblBacteriaiBAB05043; BAB05043; BAB05043
KEGGibha:BH1324

Similar proteinsi

Entry informationi

Entry nameiAROE_BACHD
AccessioniPrimary (citable) accession number: Q9KD93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health